Super-resolution fluorescence nanoscopy opportunities for EUV resist inspection and metrology (Conference Presentation)

2019 ◽  
Author(s):  
John S. Petersen
Keyword(s):  
Super Resolution ◽  
Fluorescence Nanoscopy

scholarly journals A fluorescence nanoscopy marker for corticotropin-releasing hormone type 1 receptor: computer design, synthesis, signaling effects, super-resolved fluorescence imaging, and in situ affinity constant in cells

2018 ◽  
Vol 20 (46) ◽  
pp. 29212-29220 ◽  
Author(s):  
Alan M. Szalai ◽  
Natalia G. Armando ◽  
Federico M. Barabas ◽  
Fernando D. Stefani ◽  
Luciana Giordano ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Super Resolution ◽  
Affinity Constant ◽  
Computer Design ◽  
Design Synthesis ◽  
Fluorescent Marker ◽  
Fluorescence Nanoscopy ◽  
Antagonist Effect

A new fluorescent marker for CRHR1 shows an antagonist effect and suitability for super resolution fluorescence microscopy.

scholarly journals Lens-based fluorescence nanoscopy

2015 ◽  
Vol 48 (2) ◽  
pp. 178-243 ◽  
Author(s):  
Christian Eggeling ◽  
Katrin I. Willig ◽  
Steffen J. Sahl ◽  
Stefan W. Hell
Keyword(s):  
Spatial Resolution ◽  
Living Cell ◽  
Three Dimensional ◽  
Super Resolution ◽  
Optical Microscope ◽  
Live Cells ◽  
Far Field ◽  
Resolution Limit ◽  
Advantages And Disadvantages ◽  
Fluorescence Nanoscopy

AbstractThe majority of studies of the living cell rely on capturing images using fluorescence microscopy. Unfortunately, for centuries, diffraction of light was limiting the spatial resolution in the optical microscope: structural and molecular details much finer than about half the wavelength of visible light (~200 nm) could not be visualized, imposing significant limitations on this otherwise so promising method. The surpassing of this resolution limit in far-field microscopy is currently one of the most momentous developments for studying the living cell, as the move from microscopy to super-resolution microscopy or ‘nanoscopy’ offers opportunities to study problems in biophysical and biomedical research at a new level of detail. This review describes the principles and modalities of present fluorescence nanoscopes, as well as their potential for biophysical and cellular experiments. All the existing nanoscopy variants separate neighboring features by transiently preparing their fluorescent molecules in states of different emission characteristics in order to make the features discernible. Usually these are fluorescent ‘on’ and ‘off’ states causing the adjacent molecules to emit sequentially in time. Each of the variants can in principle reach molecular spatial resolution and has its own advantages and disadvantages. Some require specific transitions and states that can be found only in certain fluorophore subfamilies, such as photoswitchable fluorophores, while other variants can be realized with standard fluorescent labels. Similar to conventional far-field microscopy, nanoscopy can be utilized for dynamical, multi-color and three-dimensional imaging of fixed and live cells, tissues or organisms. Lens-based fluorescence nanoscopy is poised for a high impact on future developments in the life sciences, with the potential to help solve long-standing quests in different areas of scientific research.

Super-Resolution Microscopy in Studying the Structure and Function of the Cell Nucleus

Acta Naturae ◽  
2017 ◽  
Vol 9 (4) ◽  
pp. 42-51
Author(s):  
S. S. Ryabichko ◽  
◽  
A. N. Ibragimov ◽  
L. A. Lebedeva ◽  
E. N. Kozlov ◽  
...  
Keyword(s):  
Cell Nucleus ◽  
Super Resolution ◽  
Structure And Function ◽  
And Function ◽  
Super Resolution Microscopy

Facial Image Super-Resolution Reconstruction Based on Separated Frequency Components

2013 ◽  
Vol E96.A (6) ◽  
pp. 1315-1322 ◽  
Author(s):  
Hyunduk KIM ◽  
Sang-Heon LEE ◽  
Myoung-Kyu SOHN ◽  
Dong-Ju KIM ◽  
Byungmin KIM
Keyword(s):  
Super Resolution ◽  
Facial Image ◽  
Frequency Components ◽  
Image Super Resolution
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