Scattered light contrast microscopy for reflectance imaging of thick tissue (Conference Presentation)

2019 ◽  
Author(s):  
Jeremy D. Rogers
Keyword(s):  
Scattered Light ◽  
Contrast Microscopy ◽  
Light Contrast

Defocused differential interference contrast microscopy imaging of single plasmonic anisotropic nanoparticles

2014 ◽  
Vol 50 (41) ◽  
pp. 5500-5502 ◽  
Author(s):  
Ji Won Ha ◽  
Ning Fang
Keyword(s):  
Spatial Orientation ◽  
Scattered Light ◽  
Gold Nanorod ◽  
Differential Interference Contrast ◽  
Differential Interference Contrast Microscopy ◽  
Microscopy Imaging ◽  
Anisotropic Nanoparticles ◽  
Contrast Microscopy ◽  
Interference Contrast Microscopy

The scattered light from a gold nanorod aligned closer to the polarization directions enables us to resolve its spatial orientation under a defocused DIC microscope.

Freeze-substituted fungal cells of Nectria haematococca: A comparison of the macroconidial walls of the adhesion-competent wild type with an adhesion-reduced mutant

1990 ◽  
Vol 48 (3) ◽  
pp. 688-689
Author(s):  
Thecan Caesar-Ton That ◽  
Lynn Epstein
Keyword(s):  
Freeze Substitution ◽  
Uranyl Acetate ◽  
Wild Type ◽  
Nectria Haematococca ◽  
Fruit Extract ◽  
Dialysis Tubing ◽  
Tube Emergence ◽  
Contrast Microscopy ◽  
In The Wild ◽  
Freeze Damage

Nectria haematococca mating population I (anamorph, Fusarium solani) macroconidia attach to its host (squash) and non-host surfaces prior to germ tube emergence. The macroconidia become adhesive after a brief period of protein synthesis. Recently, Hickman et al. (1989) isolated N. haematococca adhesion-reduced mutants. Using freeze substitution, we compared the development of the macroconidial wall in the wild type in comparison to one of the mutants, LEI.Macroconidia were harvested at 1C, washed by centrifugation, resuspended in a dilute zucchini fruit extract and incubated from 0 - 5 h. During the incubation period, wild type macroconidia attached to uncoated dialysis tubing. Mutant macroconidia did not attach and were collected on poly-L-lysine coated dialysis tubing just prior to freezing. Conidia on the tubing were frozen in liquid propane at 191 - 193C, substituted in acetone with 2% OsO4 and 0.05% uranyl acetate, washed with acetone, and flat-embedded in Epon-Araldite. Using phase contrast microscopy at 1000X, cells without freeze damage were selected, remounted, sectioned and post-stained sequentially with 1% Ba(MnO4)2 2% uranyl acetate and Reynold’s lead citrate. At least 30 cells/treatment were examined.

In-vitro- und In-vivo-Wirkung von Schilddrüsenhormon auf das Wachstum von Neuroblastomzellen

1990 ◽  
Vol 29 (03) ◽  
pp. 120-124
Author(s):  
R. P. Baum ◽  
E. Rohrbach ◽  
G. Hör ◽  
B. Kornhuber ◽  
E. Busse
Keyword(s):  
Cell Differentiation ◽  
Neuroblastoma Cells ◽  
Control Group ◽  
Initial Value ◽  
Initial Rise ◽  
Biphasic Effect ◽  
Contrast Microscopy ◽  
Morphological Sign

The effect of triiodothyronine (T3) on the differentiation of cultured neuroblastoma (NB) cells was studied after 9 days of treatment with a dose of 10-4 M/106 cells per day. Using phase contrast microscopy, 30-50% of NB cells showed formation of neurites as a morphological sign of cellular differentiation. The initial rise of the mitosis rate was followed by a plateau. Changes in cyclic nucleotide content, in the triphosphates and in the activity of the enzyme ornithine decarboxylase (ODC) were assessed in 2 human and 2 murine cell lines to serve as biochemical parameters of the cell differentiation induced by T3. Whereas the cAMP level increased significantly (3 to 7 fold compared with its initial value), the cGMP value dropped to 30 to 50% of that of the control group. ATP and GTP increased about 200%, the ODC showed a decrease of about 50%. The present studies show a biphasic effect of T3 on neuroblastoma cells: the initial rise of mitotic activity is followed by increased cell differentiation starting from day 4 of the treatment.

Magnetic resonance in the noise in the intensity of scattered light

1987 ◽  
Vol 153 (10) ◽  
pp. 363 ◽  
Author(s):  
Evgenii B. Aleksandrov ◽  
V.S. Zapasskii
Keyword(s):  
Magnetic Resonance ◽  
Scattered Light

Detachment of biofilm bacteria due to variations in nutrient supply

1998 ◽  
Vol 37 (4-5) ◽  
pp. 211-214 ◽  
Author(s):  
Linda K. Sawyer ◽  
Slawomir W. Hermanowicz
Keyword(s):  
Phase Contrast ◽  
Digital Image Analysis ◽  
Specific Growth ◽  
Nutrient Supply ◽  
Surface Shear Stress ◽  
Surface Shear ◽  
Nutrient Limitations ◽  
Contrast Microscopy ◽  
Biofilm Bacteria ◽  
Observable Parameter

Growth and detachment rates of an environmental isolate of Aeromonas hydrophila attached to a surface were determined under varying nutrient supply conditions in a complex medium. Growth and detachment of cells were observed in real time using phase contrast microscopy in glass parallel plate flow chambers. Surface shear stress was controlled in all experiments at 3 N m−2. Images were taken every 15 min. Digital image analysis was used to determine specific growth and detachment rates. An observable parameter proportional to the nutrient depletion at the surface due to transfer limitations was used to indicate nutrient limitations. Specific detachment rates increased as the depletion parameter increased, indicating that nutrient limitations cause this bacterium to detach at greater rates.

A Time-Resolved Low-Angle Light Scattering Apparatus. Application to Phase Separation Problems in Polymer Systems

2001 ◽  
Vol 66 (6) ◽  
pp. 973-982 ◽  
Author(s):  
Čestmír Koňák ◽  
Jaroslav Holoubek ◽  
Petr Štěpánek
Keyword(s):  
Phase Separation ◽  
Light Scattering ◽  
Signal To Noise Ratio ◽  
Scattered Light ◽  
Ccd Camera ◽  
Time Resolved ◽  
Polymer Systems ◽  
Software Analysis ◽  
Phase Separation Kinetics ◽  
Small Angle Light Scattering

A time-resolved small-angle light scattering apparatus equipped with azimuthal integration by means of a conical lens or software analysis of scattering patterns detected with a CCD camera was developed. Averaging allows a significant reduction of the signal-to-noise ratio of scattered light and makes this technique suitable for investigation of phase separation kinetics. Examples of applications to time evolution of phase separation in concentrated statistical copolymer solutions and dissolution of phase-separated domains in polymer blends are given.

Sign in / Sign up

Export Citation Format

Share Document