scholarly journals Co-timing UV and IR laser pulses on the OMEGA EP laser system

Author(s):  
William R. Donaldson ◽  
Albert Consentino
Keyword(s):  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Anthony D. Fouad ◽  
Alice Liu ◽  
Angelica Du ◽  
Priya D. Bhirgoo ◽  
Christopher Fang-Yen

AbstractLaser microsurgery has long been an important means of assessing the functions of specific cells and tissues. Most laser ablation systems use short, highly focused laser pulses to create plasma-mediated lesions with dimensions on the order of the wavelength of light. While the small size of the lesion enables ablation with high spatial resolution, it also makes it difficult to ablate larger structures. We developed an infrared laser ablation system capable of thermally lesioning tissues with spot sizes tunable by the duration and amplitude of laser pulses. We used our laser system in the roundworm C. elegans to kill single neurons and to sever the dorsal and ventral nerve cords, structures that are difficult to lesion using a plasma-based ablation system. We used these ablations to investigate the source of convulsions in a gain-of-function mutant for the acetylcholine receptor ACR-2. Severing the ventral nerve cord caused convulsions to occur independently anterior and posterior to the lesion, suggesting that convulsions can arise independently from distinct subsets of the motor circuit.


2017 ◽  
Vol 25 (22) ◽  
pp. 27706 ◽  
Author(s):  
V. Gruson ◽  
G. Ernotte ◽  
P. Lassonde ◽  
A. Laramée ◽  
M. R. Bionta ◽  
...  

2021 ◽  
Vol 28 (3) ◽  
Author(s):  
Matthias Rössle ◽  
Wolfram Leitenberger ◽  
Matthias Reinhardt ◽  
Azize Koç ◽  
Jan Pudell ◽  
...  

The time-resolved hard X-ray diffraction endstation KMC-3 XPP for optical pump/X-ray probe experiments at the electron storage ring BESSY II is dedicated to investigating the structural response of thin film samples and heterostructures after their excitation with ultrashort laser pulses and/or electric field pulses. It enables experiments with access to symmetric and asymmetric Bragg reflections via a four-circle diffractometer and it is possible to keep the sample in high vacuum and vary the sample temperature between ∼15 K and 350 K. The femtosecond laser system permanently installed at the beamline allows for optical excitation of the sample at 1028 nm. A non-linear optical setup enables the sample excitation also at 514 nm and 343 nm. A time-resolution of 17 ps is achieved with the `low-α' operation mode of the storage ring and an electronic variation of the delay between optical pump and hard X-ray probe pulse conveniently accesses picosecond to microsecond timescales. Direct time-resolved detection of the diffracted hard X-ray synchrotron pulses use a gated area pixel detector or a fast point detector in single photon counting mode. The range of experiments that are reliably conducted at the endstation and that detect structural dynamics of samples excited by laser pulses or electric fields are presented.


2008 ◽  
Vol 5 (3) ◽  
pp. 457-459
Author(s):  
Baghdad Science Journal

We report here the observation of 16 µm superradiance laser action generated from optical pumping of CF4 gas molecules (which is cooled to 140 Kº by a boil-off liquid-N2) by a TEA-CO2 laser 9R12 line. Output laser pulses of 7 mJ and 200 ns have been obtained.


Author(s):  
E. Guillaume ◽  
K. Humphrey ◽  
H. Nakamura ◽  
R. M. G. M. Trines ◽  
R. Heathcote ◽  
...  

Abstract The energy transfer by stimulated Brillouin backscatter from a long pump pulse (15 ps) to a short seed pulse (1 ps) has been investigated in a proof-of-principle demonstration experiment. The two pulses were both amplified in different beamlines of a Nd:glass laser system, had a central wavelength of 1054 nm and a spectral bandwidth of 2 nm, and crossed each other in an underdense plasma in a counter-propagating geometry, off-set by $\def \xmlpi #1{}\def \mathsfbi #1{\boldsymbol {\mathsf {#1}}}\let \le =\leqslant \let \leq =\leqslant \let \ge =\geqslant \let \geq =\geqslant \def \Pr {\mathit {Pr}}\def \Fr {\mathit {Fr}}\def \Rey {\mathit {Re}}10^\circ $ . It is shown that the energy transfer and the wavelength of the generated Brillouin peak depend on the plasma density, the intensity of the laser pulses, and the competition between two-plasmon decay and stimulated Raman scatter instabilities. The highest obtained energy transfer from pump to probe pulse is 2.5%, at a plasma density of $0.17 n_{cr}$ , and this energy transfer increases significantly with plasma density. Therefore, our results suggest that much higher efficiencies can be obtained when higher densities (above $0.25 n_{cr}$ ) are used.


2006 ◽  
Vol 59 (2) ◽  
pp. 109 ◽  
Author(s):  
Nina Morgner ◽  
Hans-Dieter Barth ◽  
Bernhard Brutschy

A new version of laser mass-spectrometry is presented, which allows the quantitative analysis of specific biocomplexes in native solution. On-demand micro droplets, injected into vacuum, are irradiated by mid IR-laser pulses. Above a certain intensity threshold they explode due to the transmitted energy, setting free a fraction of the charged biomolecules which are then mass-analyzed. Amounts of analyte in the attomolar range may be detected with the ion intensity being linear over a wide range of molarity. Evidence is given that this method is soft, tolerant against various buffers, reflects properties of the liquid phase, and suitable for studying noncovalently bonded specific complexes. This is highlighted by results from antibiotics specifically binding into the minor groove of duplex DNA.


1998 ◽  
Vol 4 (S2) ◽  
pp. 424-425
Author(s):  
G.Y. Fan ◽  
H. Fujisaki ◽  
R.-K. Tsay ◽  
R.Y. Tsien ◽  
Mark H. Ellisman

A video-rate scanning two-photon excitation microscope (TPEM) has been successfully constructed and tested. The TPEM, based on a Nikon RCM-8000, incorporates a femtosecond pulsed laser, a pre-chirper, and a non-confocal detection box for ratio imaging. Fig. 1 shows the schematic layout of the main components of the instrument, each of which is briefly discussed below.Laser System: A Tsunami Ti: Sapphire laser (from Spectra-Physics) is optically pumped by a 5 W green laser (Millennia from Spectra-Physics) and is capable of generating 100 fs pulses at a repetition rate of 82 MHz and an average power of 0.8 W. The output wavelength is tunable from 690 to 1050 nm with three optical sets, each covering part of the spectrum with some overlapping.Pre-chirper: After leaving the Tsunami, the laser beam enters an optic unit known as a pre-chirper which pre-chirps laser pulses to compensate for the group velocity dispersion which will result when the laser beam goes through the microscope optics.


2018 ◽  
Vol 48 (3) ◽  
pp. 244-250 ◽  
Author(s):  
T V Kononenko ◽  
P A Pivovarov ◽  
A A Khomich ◽  
R A Khmel'nitskii ◽  
V I Konov
Keyword(s):  

2001 ◽  
Vol 42 (6) ◽  
pp. 535-540
Author(s):  
Akshaya K Nayak ◽  
Venkatachari Parthasarathy ◽  
Sisir K Sarkar

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