A New Way To Detect Noncovalently Bonded Complexes of Biomolecules from Liquid Micro-Droplets by Laser Mass Spectrometry

2006 ◽  
Vol 59 (2) ◽  
pp. 109 ◽  
Author(s):  
Nina Morgner ◽  
Hans-Dieter Barth ◽  
Bernhard Brutschy
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Liquid Phase ◽  
Laser Pulses ◽  
Laser Mass Spectrometry ◽  
Native Solution ◽  
On Demand ◽  
Ir Laser ◽  
Intensity Threshold ◽  
Wide Range

A new version of laser mass-spectrometry is presented, which allows the quantitative analysis of specific biocomplexes in native solution. On-demand micro droplets, injected into vacuum, are irradiated by mid IR-laser pulses. Above a certain intensity threshold they explode due to the transmitted energy, setting free a fraction of the charged biomolecules which are then mass-analyzed. Amounts of analyte in the attomolar range may be detected with the ion intensity being linear over a wide range of molarity. Evidence is given that this method is soft, tolerant against various buffers, reflects properties of the liquid phase, and suitable for studying noncovalently bonded specific complexes. This is highlighted by results from antibiotics specifically binding into the minor groove of duplex DNA.

Quantitative analysis of mixed benzalkonium chlorides by laser mass spectrometry

1983 ◽  
Vol 55 (1) ◽  
pp. 145-146 ◽  
Author(s):  
Kesagapillai. Balasanmugam ◽  
David M. Hercules
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Laser Mass Spectrometry

Precision and bias in quantitative EDS: ASTM results

1992 ◽  
Vol 50 (2) ◽  
pp. 1654-1655
Author(s):  
John J. Friel
Keyword(s):  
Stainless Steel ◽  
Quantitative Analysis ◽  
Mechanical Alloy ◽  
Dead Time ◽  
Test Program ◽  
Round Robin Test ◽  
Wide Range ◽  
American Society ◽  
Takeoff Angle ◽  
Standardless Analysis

Committee E-04 on Metallography of the American Society for Testing and Materials (ASTM) conducted an interlaboratory round robin test program on quantitative energy dispersive spectroscopy (EDS). The test program was designed to produce data on which to base a precision and bias statement for quantitative analysis by EDS. Nine laboratories were sent specimens of two well characterized materials, a type 308 stainless steel, and a complex mechanical alloy from Inco Alloys International, Inconel® MA 6000. The stainless steel was chosen as an example of a straightforward analysis with no special problems. The mechanical alloy was selected because elements were present in a wide range of concentrations; K, L, and M lines were involved; and Ta was severely overlapped with W. The test aimed to establish limits of precision that could be routinely achieved by capable laboratories operating under real world conditions. The participants were first allowed to use their own best procedures, but later were instructed to repeat the analysis using specified conditions: 20 kV accelerating voltage, 200s live time, ∼25% dead time and ∼40° takeoff angle. They were also asked to run a standardless analysis.

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