Simultaneous measurement of Cy5.5-labeled capture antibodies and Cy5-labeled antigens in a fiber optic biosensor: photobleaching and resonance energy transfer effects during fiber regeneration

1996 ◽  
Author(s):  
Randy R. Wadkins ◽  
Joel P. Golden ◽  
Frances S. Ligler
2017 ◽  
Vol 5 (24) ◽  
pp. 12120-12130 ◽  
Author(s):  
Pengqing Bi ◽  
Fei Zheng ◽  
Xiaoyu Yang ◽  
Mengsi Niu ◽  
Lin Feng ◽  
...  

An investigation of phase distribution demonstrated that PCDTBT was embedded in PTB7-Th and ITIC, and hence introduced dual FRET effects in the resulting ternary system.


Author(s):  
Alba Calatayud-Sanchez ◽  
Raquel Catalan-Carrio ◽  
Ángel Ortega ◽  
Javier Barroso Lázaro ◽  
Joseba Zubia ◽  
...  

2003 ◽  
Vol 773 ◽  
Author(s):  
Aaron R. Clapp ◽  
Igor L. Medintz ◽  
J. Matthew Mauro ◽  
Hedi Mattoussi

AbstractLuminescent CdSe-ZnS core-shell quantum dot (QD) bioconjugates were used as energy donors in fluorescent resonance energy transfer (FRET) binding assays. The QDs were coated with saturating amounts of genetically engineered maltose binding protein (MBP) using a noncovalent immobilization process, and Cy3 organic dyes covalently attached at a specific sequence to MBP were used as energy acceptor molecules. Energy transfer efficiency was measured as a function of the MBP-Cy3/QD molar ratio for two different donor fluorescence emissions (different QD core sizes). Apparent donor-acceptor distances were determined from these FRET studies, and the measured distances are consistent with QD-protein conjugate dimensions previously determined from structural studies.


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