<title>Quantitative analysis of blue noise mask generation</title>

Quantitative image analysis systems have been used for several years in research and quality control applications in various fields including metallurgy and medicine. The technique has been applied as an extension of subjective microscopy to problems requiring quantitative results and which are amenable to automatic methods of interpretation.Feature extraction. In the most general sense, a feature can be defined as a portion of the image which differs in some consistent way from the background. A feature may be characterized by the density difference between itself and the background, by an edge gradient, or by the spatial frequency content (texture) within its boundaries. The task of feature extraction includes recognition of features and encoding of the associated information for quantitative analysis.Quantitative Analysis. Quantitative analysis is the determination of one or more physical measurements of each feature. These measurements may be straightforward ones such as area, length, or perimeter, or more complex stereological measurements such as convex perimeter or Feret's diameter.

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Quantification of Silica Uptake by Alveolar Macrophages - An Emperical Scanning Electron Microprobe Method

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100054297 ◽

1982 ◽

Vol 40 ◽

pp. 332-333

Author(s):

V. V. Damiano ◽

R. P. Daniele ◽

H. T. Tucker ◽

J. H. Dauber

Keyword(s):

Quantitative Analysis ◽

Alveolar Macrophages ◽

Bulk Sample ◽

Silica Particles ◽

Empirical Method ◽

Phagocytic Cells ◽

Calibration Standards ◽

X Ray ◽

Accurate Quantitative Analysis ◽

Scanning Electron

An important example of intracellular particles is encountered in silicosis where alveolar macrophages ingest inspired silica particles. The quantitation of the silica uptake by these cells may be a potentially useful method for monitoring silica exposure. Accurate quantitative analysis of ingested silica by phagocytic cells is difficult because the particles are frequently small, irregularly shaped and cannot be visualized within the cells. Semiquantitative methods which make use of particles of known size, shape and composition as calibration standards may be the most direct and simplest approach to undertake. The present paper describes an empirical method in which glass microspheres were used as a model to show how the ratio of the silicon Kα peak X-ray intensity from the microspheres to that of a bulk sample of the same composition correlated to the mass of the microsphere contained within the cell. Irregular shaped silica particles were also analyzed and a calibration curve was generated from these data.

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