High speed 3D two-photon fluorescence microscopy by femtosecond laser pulses

2017 ◽  
Author(s):  
Meishan Guo ◽  
Yifan Qin ◽  
Sheng Zhang ◽  
Shixin Wang ◽  
Yuanqin Xia
Keyword(s):  
Fluorescence Microscopy ◽  
Femtosecond Laser ◽  
High Speed ◽  
Laser Pulses ◽  
Femtosecond Laser Pulses ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Multifarious Two-Photon Fluorescence Microscopy Employing Ultrabroadband Femtosecond Laser Pulses

2009 ◽  
Author(s):  
Keisuke Isobe ◽  
Akira Suda ◽  
Hiroshi Hashimoto ◽  
Fumihiko Kannari ◽  
Hiroyuki Kawano ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Femtosecond Laser ◽  
Laser Pulses ◽  
Femtosecond Laser Pulses ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals 2G1515 Suppression of photobleaching of EGFP two-photon fluorescence by using chirped femtosecond laser pulses

2002 ◽  
Vol 42 (supplement2) ◽  
pp. S116
Author(s):  
H. Kawano ◽  
Y. Nabekawa ◽  
A. Suda ◽  
Y. Oishi ◽  
H. Mizuno ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Laser Pulses ◽  
Femtosecond Laser Pulses ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals High-speed label-free two-photon fluorescence microscopy of metabolic transients during neuronal activity

2021 ◽  
Vol 118 (8) ◽  
pp. 081104
Author(s):  
Andrew J. Bower ◽  
Carlos Renteria ◽  
Joanne Li ◽  
Marina Marjanovic ◽  
Ronit Barkalifa ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Neuronal Activity ◽  
High Speed ◽  
Label Free ◽  
Two Photon ◽  
Metabolic Transients ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Femtosecond Laser-Excited Two-Photon Fluorescence Microscopy of Surface Plasmon Polariton

2012 ◽  
Vol 51 (4S) ◽  
pp. 04DG03 ◽  
Author(s):  
Tatsumi Hattori ◽  
Atsushi Kubo ◽  
Katsuya Oguri ◽  
Hidetoshi Nakano ◽  
Hideki T. Miyazaki
Keyword(s):  
Fluorescence Microscopy ◽  
Femtosecond Laser ◽  
Surface Plasmon ◽  
Surface Plasmon Polariton ◽  
Two Photon ◽  
Plasmon Polariton ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Femtosecond Laser-Excited Two-Photon Fluorescence Microscopy of Surface Plasmon Polariton

2012 ◽  
Vol 51 ◽  
pp. 04DG03 ◽  
Author(s):  
Tatsumi Hattori ◽  
Atsushi Kubo ◽  
Katsuya Oguri ◽  
Hidetoshi Nakano ◽  
Hideki T. Miyazaki
Keyword(s):  
Fluorescence Microscopy ◽  
Femtosecond Laser ◽  
Surface Plasmon ◽  
Surface Plasmon Polariton ◽  
Two Photon ◽  
Plasmon Polariton ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Live cell bioimaging with carbon dots produced in situ by femtosecond laser from intracellular material

2018 ◽  
Author(s):  
Aleksander M. Shakhov ◽  
Artyom A. Astafiev ◽  
Alina A. Osychenko ◽  
Maria S. Syrchina ◽  
Viktor A. Nadtochenko
Keyword(s):  
Femtosecond Laser ◽  
Carbon Dots ◽  
Near Infrared ◽  
Laser Pulses ◽  
Culture Cell ◽  
Femtosecond Laser Pulses ◽  
Live Cell ◽  
Two Photon ◽  
Two Photon Fluorescence

Owning to excellent optical properties and high biocompatibility carbon dots (CDs) have drawn increasing attention and have been widely applied as imaging agents for various bio-applications. Here we report a strategy for live-cell fluorescent bioimaging based on in situ synthesis of CDs within cells by tightly focused femtosecond laser pulses. Laser-produced carbon dots exhibit bright excitation-dependent fluorescence and are highly two-photon active under near infrared femtosecond excitation, thus demonstrating a potential for two-photon fluorescence imaging. The Raman spectra of fluorescent centers show strong D (1350 cm-1) and G (1590 cm-1) bands, thus suggesting that they are composed of carbon dots with sp2-hybridized core. Using Mouse GV oocytes as a model system we examine cytotoxicity and demonstrate the possibility of long-term fluorescent intracellular tracking of the laser-produced CDs. Created virtually in any point of the live cell, CD-based fluorescent μm-sized markers demonstrate high structural stability and retain bright fluorescence many hours after formation. Our results point to laser-produced fluorescent CDs as a highly-potent tool for cell cycle tracking, culture cell marking and probing intracellular movements.

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