Multifarious Two-Photon Fluorescence Microscopy Employing Ultrabroadband Femtosecond Laser Pulses

2009 ◽  
Author(s):  
Keisuke Isobe ◽  
Akira Suda ◽  
Hiroshi Hashimoto ◽  
Fumihiko Kannari ◽  
Hiroyuki Kawano ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Femtosecond Laser ◽  
Laser Pulses ◽  
Femtosecond Laser Pulses ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals 2G1515 Suppression of photobleaching of EGFP two-photon fluorescence by using chirped femtosecond laser pulses

2002 ◽  
Vol 42 (supplement2) ◽  
pp. S116
Author(s):  
H. Kawano ◽  
Y. Nabekawa ◽  
A. Suda ◽  
Y. Oishi ◽  
H. Mizuno ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Laser Pulses ◽  
Femtosecond Laser Pulses ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Femtosecond Laser-Excited Two-Photon Fluorescence Microscopy of Surface Plasmon Polariton

2012 ◽  
Vol 51 (4S) ◽  
pp. 04DG03 ◽  
Author(s):  
Tatsumi Hattori ◽  
Atsushi Kubo ◽  
Katsuya Oguri ◽  
Hidetoshi Nakano ◽  
Hideki T. Miyazaki
Keyword(s):  
Fluorescence Microscopy ◽  
Femtosecond Laser ◽  
Surface Plasmon ◽  
Surface Plasmon Polariton ◽  
Two Photon ◽  
Plasmon Polariton ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Femtosecond Laser-Excited Two-Photon Fluorescence Microscopy of Surface Plasmon Polariton

2012 ◽  
Vol 51 ◽  
pp. 04DG03 ◽  
Author(s):  
Tatsumi Hattori ◽  
Atsushi Kubo ◽  
Katsuya Oguri ◽  
Hidetoshi Nakano ◽  
Hideki T. Miyazaki
Keyword(s):  
Fluorescence Microscopy ◽  
Femtosecond Laser ◽  
Surface Plasmon ◽  
Surface Plasmon Polariton ◽  
Two Photon ◽  
Plasmon Polariton ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Live cell bioimaging with carbon dots produced in situ by femtosecond laser from intracellular material

2018 ◽  
Author(s):  
Aleksander M. Shakhov ◽  
Artyom A. Astafiev ◽  
Alina A. Osychenko ◽  
Maria S. Syrchina ◽  
Viktor A. Nadtochenko
Keyword(s):  
Femtosecond Laser ◽  
Carbon Dots ◽  
Near Infrared ◽  
Laser Pulses ◽  
Culture Cell ◽  
Femtosecond Laser Pulses ◽  
Live Cell ◽  
Two Photon ◽  
Two Photon Fluorescence

Owning to excellent optical properties and high biocompatibility carbon dots (CDs) have drawn increasing attention and have been widely applied as imaging agents for various bio-applications. Here we report a strategy for live-cell fluorescent bioimaging based on in situ synthesis of CDs within cells by tightly focused femtosecond laser pulses. Laser-produced carbon dots exhibit bright excitation-dependent fluorescence and are highly two-photon active under near infrared femtosecond excitation, thus demonstrating a potential for two-photon fluorescence imaging. The Raman spectra of fluorescent centers show strong D (1350 cm-1) and G (1590 cm-1) bands, thus suggesting that they are composed of carbon dots with sp2-hybridized core. Using Mouse GV oocytes as a model system we examine cytotoxicity and demonstrate the possibility of long-term fluorescent intracellular tracking of the laser-produced CDs. Created virtually in any point of the live cell, CD-based fluorescent μm-sized markers demonstrate high structural stability and retain bright fluorescence many hours after formation. Our results point to laser-produced fluorescent CDs as a highly-potent tool for cell cycle tracking, culture cell marking and probing intracellular movements.

Inorganic-organic Hybrid Materials for Real 3-D Sub-νm Lithography

2003 ◽  
Vol 780 ◽  
Author(s):  
R. Houbertz ◽  
J. Schulz ◽  
L. Fröhlich ◽  
G. Domann ◽  
M. Popall ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Sol Gel ◽  
Laser Pulses ◽  
Femtosecond Laser Pulses ◽  
Hybrid Polymer ◽  
Organic Hybrid ◽  
Two Photon ◽  
Applied Technology ◽  
Scale Structures ◽  
Sol Gel Synthesis

AbstractReal 3-D sub-νm lithography was performed with two-photon polymerization (2PP) using inorganic-organic hybrid polymer (ORMOCER®) resins. The hybrid polymers were synthesized by hydrolysis/polycondensation reactions (modified sol-gel synthesis) which allows one to tailor their material properties towards the respective applications, i.e., dielectrics, optics or passivation. Due to their photosensitive organic functionalities, ORMOCER®s can be patterned by conventional photo-lithography as well as by femtosecond laser pulses at 780 nm. This results in polymerized (solid) structures where the non-polymerized parts can be removed by conventional developers.ORMOCER® structures as small as 200 nm or even below were generated by 2PP of the resins using femtosecond laser pulses. It is demonstrated that ORMOCER®s have the potential to be used in components or devices built up by nm-scale structures such as, e.g., photonic crystals. Aspects of the materials in conjunction to the applied technology are discussed.

Picosecond Single-Photon and Femtosecond Two-Photon Pulsed Laser Stimulation for IC Characterization and Failure Analysis

2009 ◽  
Author(s):  
V. Pouget ◽  
E. Faraud ◽  
K. Shao ◽  
S. Jonathas ◽  
D. Horain ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Single Photon ◽  
Pulsed Laser ◽  
Laser Pulses ◽  
Femtosecond Laser Pulses ◽  
Ultrashort Laser Pulses ◽  
Laser Stimulation ◽  
Two Photon ◽  
Resolution Improvement ◽  
Ultrashort Laser

Abstract This paper presents the use of pulsed laser stimulation with picosecond and femtosecond laser pulses. We first discuss the resolution improvement that can be expected when using ultrashort laser pulses. Two case studies are then presented to illustrate the possibilities of the pulsed laser photoelectric stimulation in picosecond single-photon and femtosecond two-photon modes.

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