Photonic time stretch to measure small spectral changes with broad wavelength coverage for high-speed coherent Raman microscopy (Conference Presentation)

2018 ◽  
Author(s):  
Francesco Saltarelli ◽  
Vikas Kumar ◽  
Daniele Viola ◽  
Francesco Crisafi ◽  
Antonio Perri ◽  
...  
Keyword(s):  
High Speed ◽  
Raman Microscopy ◽  
Spectral Changes ◽  
Coherent Raman

scholarly journals Coherent Raman Scattering Microscopy in Oncology Pharmacokinetic Research

2021 ◽  
Vol 12 ◽  
Author(s):  
Junjie Zeng ◽  
Wenying Zhao ◽  
Shuhua Yue
Keyword(s):  
Raman Scattering ◽  
High Speed ◽  
Cancer Drug ◽  
Cancer Drugs ◽  
High Speed Imaging ◽  
Coherent Raman Scattering ◽  
Anti Cancer ◽  
Coherent Raman

The high attrition rates of anti-cancer drugs during clinical development remains a bottleneck problem in pharmaceutical industry. This is partially due to the lack of quantitative, selective, and rapid readouts of anti-cancer drug activity in situ with high resolution. Although fluorescence microscopy has been commonly used in oncology pharmacological research, fluorescent labels are often too large in size for small drug molecules, and thus may disturb the function or metabolism of these molecules. Such challenge can be overcome by coherent Raman scattering microscopy, which is capable of chemically selective, highly sensitive, high spatial resolution, and high-speed imaging, without the need of any labeling. Coherent Raman scattering microscopy has tremendously improved the understanding of pharmaceutical materials in the solid state, pharmacokinetics of anti-cancer drugs and nanocarriers in vitro and in vivo. This review focuses on the latest applications of coherent Raman scattering microscopy as a new emerging platform to facilitate oncology pharmacokinetic research.

Coherent Raman Microscopy with a Fiber-Format Femtosecond Laser Oscillator

2010 ◽  
Author(s):  
M. Marangoni ◽  
A. Gambetta ◽  
V. Kumar ◽  
G. Grancini ◽  
D. Polli ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Raman Microscopy ◽  
Laser Oscillator ◽  
Coherent Raman

Fiber-format Picosecond Source for Coherent Raman Microscopy

2012 ◽  
Author(s):  
Simon Lefrancois ◽  
Lingjie Kong ◽  
William Wadsworth ◽  
Robert Herda ◽  
Armin Zach ◽  
...  
Keyword(s):  
Raman Microscopy ◽  
Coherent Raman

Coherent Raman Microscopy with a Fiber-Format Femtosecond Laser Oscillator

2010 ◽  
Author(s):  
A. Gambetta ◽  
V. Kumar ◽  
G. Grancini ◽  
D. Polli ◽  
C. Manzoni ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Raman Microscopy ◽  
Laser Oscillator ◽  
Coherent Raman

scholarly journals Lipid Order Degradation in Autoimmune Demyelination Probed by Polarized Coherent Raman Microscopy

2017 ◽  
Vol 113 (7) ◽  
pp. 1520-1530 ◽  
Author(s):  
Paulina Gasecka ◽  
Alexandre Jaouen ◽  
Fatma-Zohra Bioud ◽  
Hilton B. de Aguiar ◽  
Julien Duboisset ◽  
...  
Keyword(s):  
Raman Microscopy ◽  
Lipid Order ◽  
Coherent Raman ◽  
Autoimmune Demyelination

scholarly journals High-speed polarization-resolved coherent Raman scattering imaging

Optica ◽  
2017 ◽  
Vol 4 (7) ◽  
pp. 795 ◽  
Author(s):  
Matthias Hofer ◽  
Naveen K. Balla ◽  
Sophie Brasselet
Keyword(s):  
Raman Scattering ◽  
High Speed ◽  
Coherent Raman Scattering ◽  
Coherent Raman

scholarly journals High-speed coherent Raman fingerprint imaging of biological tissues

2014 ◽  
Vol 8 (8) ◽  
pp. 627-634 ◽  
Author(s):  
Charles H. Camp Jr ◽  
Young Jong Lee ◽  
John M. Heddleston ◽  
Christopher M. Hartshorn ◽  
Angela R. Hight Walker ◽  
...  
Keyword(s):  
High Speed ◽  
Biological Tissues ◽  
Coherent Raman

scholarly journals Quantitative mapping of triacylglycerol chain length and saturation using broadband CARS microscopy

2018 ◽  
Author(s):  
A. Paul ◽  
Y.J. Wang ◽  
C. Brännmark ◽  
S. Kumar ◽  
M. Bonn ◽  
...  
Keyword(s):  
Chain Length ◽  
High Speed ◽  
Lipid Analysis ◽  
Neutral Lipids ◽  
Cell Types ◽  
Label Free ◽  
Complex Samples ◽  
Disease States ◽  
Coherent Raman

AbstractLipid droplets (LDs) are highly dynamic organelles that store neutral lipids, primarily triacylglycerols (TAGs), and are found in many cell types. While their primary function is to store excess energy, LDs are also modified in different disease states and during developmental processes. In many cases, not only the presence, but also the composition, of LDs can be equally important. In humans, LD composition has been linked to diseases such as type 2 diabetes; in plants and yeast, LD composition is relevant for engineering these organisms into biological factories in, e.g., algal bioenergy or food oil production. Therefore, lipid analysis of biological LDs with high speed and accuracy in situ is a very active area of research. Here we present an approach for in situ, quantitative TAG analysis using label-free, coherent Raman microscopy to decipher LD TAG composition in different biochemically complex samples. Our method allows direct visualization of inter-LD compositional heterogeneity of physiological quantities – TAG chain length and number of C=C bonds – with sub-micrometer spatial resolution within 5-100 milliseconds. Combined with virtually no sample preparation, this approach should enable rapid and accurate TAG LD analysis for a variety of applications.

scholarly journals High-power picosecond fiber source for coherent Raman microscopy

2009 ◽  
Vol 34 (13) ◽  
pp. 2051 ◽  
Author(s):  
Khanh Kieu ◽  
Brian G. Saar ◽  
Gary R. Holtom ◽  
X. Sunney Xie ◽  
Frank W. Wise
Keyword(s):  
High Power ◽  
Raman Microscopy ◽  
Coherent Raman
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