scholarly journals High-speed coherent Raman fingerprint imaging of biological tissues

2014 ◽  
Vol 8 (8) ◽  
pp. 627-634 ◽  
Author(s):  
Charles H. Camp Jr ◽  
Young Jong Lee ◽  
John M. Heddleston ◽  
Christopher M. Hartshorn ◽  
Angela R. Hight Walker ◽  
...  
Keyword(s):  
High Speed ◽  
Biological Tissues ◽  
Coherent Raman

scholarly journals Advanced Label-Free Laser Scanning Microscopy and Its Biological Imaging Application

2021 ◽  
Vol 11 (3) ◽  
pp. 1002
Author(s):  
Xue Wang ◽  
Xinchao Lu ◽  
Chengjun Huang
Keyword(s):  
Laser Scanning ◽  
Second Harmonic ◽  
Biological Tissues ◽  
Biological Imaging ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Localized Surface Plasmon ◽  
Label Free ◽  
Coherent Raman ◽  
Surface Plasmon Microscopy

By eliminating the photodamage and photobleaching induced by high intensity laser and fluorescent molecular, the label-free laser scanning microscopy shows powerful capability for imaging and dynamic tracing to biological tissues and cells. In this review, three types of label-free laser scanning microscopies: laser scanning coherent Raman scattering microscopy, second harmonic generation microscopy and scanning localized surface plasmon microscopy are discussed with their fundamentals, features and recent progress. The applications of label-free biological imaging of these laser scanning microscopies are also introduced. Finally, the performance of the microscopies is compared and the limitation and perspectives are summarized.

scholarly journals Achieving High-Speed Retraction in a Stretchable Hydrogel

2020 ◽  
Author(s):  
Rosa Maria Badani Prado ◽  
Satish Mishra ◽  
Buckston Morgan ◽  
Rangana Wijayapala ◽  
Seyed Meysam Hashemnejad ◽  
...  
Keyword(s):  
High Speed ◽  
Biological Tissues ◽  
Biological Species ◽  
Vital Role ◽  
Polypropylene Glycol ◽  
Glycol Diacrylate ◽  
Mantis Shrimp ◽  
Power Amplification ◽  
Short Period ◽  
Very High

Many biological species apply the power amplification mechanism for locomotion, feeding, and protection. In power amplification, a biological system rapidly releases stored-energy by achieving a very high velocity over a short period of time, resulting in high power output. Such power amplification allows insects such as locust to jump and Mantis shrimp to kill prey by its appendage strike. Biological elastomeric polymers such as resilin play a vital role in the power amplification process because of their high stretchability and resilience. In synthetic materials, although<br>crosslinked rubbers display high stretchability and resilience, such is difficult to achieve in the water-containing systems such as in hydrogels, commonly considered materials for mimicking biological tissues. Here, we have used a simple free-radical polymerization of acrylic acid (AAc), methacrylamide (MAAm), and polypropylene glycol diacrylate (PPGDA) to obtain hydrogels. In these gels, the polymerized AAc and MAAm act as hydrophilic blocks and PPG as hydrophobic, and the gel structure resemble that of resilin consisting of hydrophilic and hydrophobic components. The bioinspired gels display very high stretchability, as high as eight times the original length, and greater than 90% resilience. In addition, the gel samples can reach a retraction velocity of 16 m/s with an acceleration of 4X10^3 m/s2. These values are similar or better than those observed in water containing biological systems, such as appendage strikes in Mantis shrimp, etc. To the best of our knowledge, such performance has not been reported in the<br>literature for any water containing networks.

scholarly journals Coherent Raman Scattering Microscopy in Oncology Pharmacokinetic Research

2021 ◽  
Vol 12 ◽  
Author(s):  
Junjie Zeng ◽  
Wenying Zhao ◽  
Shuhua Yue
Keyword(s):  
Raman Scattering ◽  
High Speed ◽  
Cancer Drug ◽  
Cancer Drugs ◽  
High Speed Imaging ◽  
Coherent Raman Scattering ◽  
Anti Cancer ◽  
Coherent Raman

The high attrition rates of anti-cancer drugs during clinical development remains a bottleneck problem in pharmaceutical industry. This is partially due to the lack of quantitative, selective, and rapid readouts of anti-cancer drug activity in situ with high resolution. Although fluorescence microscopy has been commonly used in oncology pharmacological research, fluorescent labels are often too large in size for small drug molecules, and thus may disturb the function or metabolism of these molecules. Such challenge can be overcome by coherent Raman scattering microscopy, which is capable of chemically selective, highly sensitive, high spatial resolution, and high-speed imaging, without the need of any labeling. Coherent Raman scattering microscopy has tremendously improved the understanding of pharmaceutical materials in the solid state, pharmacokinetics of anti-cancer drugs and nanocarriers in vitro and in vivo. This review focuses on the latest applications of coherent Raman scattering microscopy as a new emerging platform to facilitate oncology pharmacokinetic research.

High-speed label-free ultraviolet photoacoustic microscopy for histology-like imaging of unprocessed biological tissues

2020 ◽  
Vol 45 (19) ◽  
pp. 5401 ◽  
Author(s):  
Xiufeng Li ◽  
Lei Kang ◽  
Yan Zhang ◽  
Terence T. W. Wong
Keyword(s):  
High Speed ◽  
Biological Tissues ◽  
Photoacoustic Microscopy ◽  
Label Free

scholarly journals A DIC Based Technique to Measure the Contraction of a Skeletal Muscle Engineered Tissue

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Emanuele Rizzuto ◽  
Silvia Carosio ◽  
Martina Faraldi ◽  
Simona Pisu ◽  
Antonio Musarò ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
High Speed ◽  
Region Of Interest ◽  
Biological Tissues ◽  
Image Correlation ◽  
Spontaneous Contraction ◽  
Noninvasive Measurement ◽  
Custom Made ◽  
Engineered Tissue ◽  
Contractile Forces

Tissue engineering is a multidisciplinary science based on the application of engineering approaches to biologic tissue formation. Engineered tissue internal organization represents a key aspect to increase biofunctionality before transplant and, as regarding skeletal muscles, the potential of generating contractile forces is dependent on the internal fiber organization and is reflected by some macroscopic parameters, such as the spontaneous contraction. Here we propose the application of digital image correlation (DIC) as an independent tool for an accurate and noninvasive measurement of engineered muscle tissue spontaneous contraction. To validate the proposed technique we referred to the X-MET, a promising 3-dimensional model of skeletal muscle. The images acquired through a high speed camera were correlated with a custom-made algorithm and the longitudinal strain predictions were employed for measuring the spontaneous contraction. The spontaneous contraction reference values were obtained by studying the force response. The relative error between the spontaneous contraction frequencies computed in both ways was always lower than 0.15%. In conclusion, the use of a DIC based system allows for an accurate and noninvasive measurement of biological tissues’ spontaneous contraction, in addition to the measurement of tissue strain field on any desired region of interest during electrical stimulation.

scholarly journals High-speed polarization-resolved coherent Raman scattering imaging

Optica ◽  
2017 ◽  
Vol 4 (7) ◽  
pp. 795 ◽  
Author(s):  
Matthias Hofer ◽  
Naveen K. Balla ◽  
Sophie Brasselet
Keyword(s):  
Raman Scattering ◽  
High Speed ◽  
Coherent Raman Scattering ◽  
Coherent Raman

scholarly journals Quantitative mapping of triacylglycerol chain length and saturation using broadband CARS microscopy

2018 ◽  
Author(s):  
A. Paul ◽  
Y.J. Wang ◽  
C. Brännmark ◽  
S. Kumar ◽  
M. Bonn ◽  
...  
Keyword(s):  
Chain Length ◽  
High Speed ◽  
Lipid Analysis ◽  
Neutral Lipids ◽  
Cell Types ◽  
Label Free ◽  
Complex Samples ◽  
Disease States ◽  
Coherent Raman

AbstractLipid droplets (LDs) are highly dynamic organelles that store neutral lipids, primarily triacylglycerols (TAGs), and are found in many cell types. While their primary function is to store excess energy, LDs are also modified in different disease states and during developmental processes. In many cases, not only the presence, but also the composition, of LDs can be equally important. In humans, LD composition has been linked to diseases such as type 2 diabetes; in plants and yeast, LD composition is relevant for engineering these organisms into biological factories in, e.g., algal bioenergy or food oil production. Therefore, lipid analysis of biological LDs with high speed and accuracy in situ is a very active area of research. Here we present an approach for in situ, quantitative TAG analysis using label-free, coherent Raman microscopy to decipher LD TAG composition in different biochemically complex samples. Our method allows direct visualization of inter-LD compositional heterogeneity of physiological quantities – TAG chain length and number of C=C bonds – with sub-micrometer spatial resolution within 5-100 milliseconds. Combined with virtually no sample preparation, this approach should enable rapid and accurate TAG LD analysis for a variety of applications.

Towards high speed high sensitivity optical coherence tomography for in vivo functional imaging

2015 ◽  
Author(s):  
◽  
Miao Zhang
Keyword(s):  
Optical Coherence Tomography ◽  
High Speed ◽  
Imaging System ◽  
Biological Tissues ◽  
Dual Band ◽  
Optical Coherence ◽  
Process Data ◽  
Airy Beam ◽  
Imaging Speed

[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Optical coherence tomography (OCT) is an imaging technique that has been widely used in clinics and industrial fields. This dissertation, making use of several emerging techniques, works on improving the imaging speed and sensitivity of current OCT systems so that it will be more powerful in imaging biological tissues, live animals and human patients. Dual-band Fourier domain OCT (FD-OCT) has the potential to provide high quality images that can differentiate different types of tissues. However, previous dual-band FD-OCT systems could not give correct information due to inherited limitations in imaging system setup, sample properties and theory. Our new imaging system overcomes these limitations by using unique hardware and software design. In our imaging system, different false signals are suppressed and the signal attenuation due to samples are compensated using proposed algorithms that derived from theoretical analysis. A video card is used to process data in an ultrahigh speed. With high imaging speed we are able to imaging live animals despite the movement of subjects. The high speed also makes it possible to display the imaging volume interactively in 3D. The image quality and information contained in the images are improved. We further developed an OCT imaging system using a special kind of optical beam called finite energy Airy beam. Compared to OCT system using traditional optical beams, Airy beam OCT has the potential to view a much longer range and view deeper inside the biological tissue.

scholarly journals More from less: high-throughput dual polarity lipid imaging of biological tissues

The Analyst ◽  
2016 ◽  
Vol 141 (12) ◽  
pp. 3832-3841 ◽  
Author(s):  
Shane R. Ellis ◽  
Joanna Cappell ◽  
Nina Ogrinc Potočnik ◽  
Benjamin Balluff ◽  
Julie Hamaide ◽  
...  
Keyword(s):  
High Throughput ◽  
High Speed ◽  
Spatial Information ◽  
Biological Tissues ◽  
Tissue Sections ◽  
Dual Polarity

Here, we reveal the increased biochemical and spatial information acquired using high-speed MALDI-MSI and sequential acquisitions of positive and negative lipid-MSI data from single tissue sections.

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