Tradeoff between insensitivity to depth-induced spherical aberration and resolution of 3D fluorescence imaging due to the use of wavefront encoding with a radially symmetric phase mask

2018 ◽  
Author(s):  
Ana Doblas ◽  
Chrysanthe Preza ◽  
Ananya Dutta ◽  
Genaro Saavedra
Keyword(s):  
Fluorescence Imaging ◽  
Spherical Aberration ◽  
Phase Mask ◽  
Symmetric Phase

scholarly journals Reducing effects of aberration in 3D fluorescence imaging using wavefront coding with a radially symmetric phase mask

2016 ◽  
Vol 24 (12) ◽  
pp. 12905 ◽  
Author(s):  
Nurmohammed Patwary ◽  
Sharon V. King ◽  
Genaro Saavedra ◽  
Chrysanthe Preza
Keyword(s):  
Fluorescence Imaging ◽  
Phase Mask ◽  
Wavefront Coding ◽  
Symmetric Phase

An extended depth-of-field imaging system with a non-rotationally symmetric phase mask

2018 ◽  
Vol 89 (10) ◽  
pp. 103101 ◽  
Author(s):  
Hongbo Xie ◽  
Lirong He ◽  
Lei Yang ◽  
Chensheng Mao ◽  
Meng Zhu ◽  
...  
Keyword(s):  
Imaging System ◽  
Phase Mask ◽  
Depth Of Field ◽  
Rotationally Symmetric ◽  
Extended Depth Of Field ◽  
Field Imaging ◽  
Symmetric Phase

scholarly journals Spherical Aberration of Point Spread Function with Asymmetric Pupil Mask

2016 ◽  
Vol 2016 ◽  
pp. 1-5
Author(s):  
Naresh Kumar Reddy Andra ◽  
Karuna Sagar Dasari
Keyword(s):  
Point Spread Function ◽  
Spherical Aberration ◽  
Spherical Wave ◽  
Super Resolution ◽  
Central Peak ◽  
Phase Mask ◽  
One Dimensional ◽  
Point Spread ◽  
Spread Function ◽  
Wave Aberration

Point spread function underneath spherical wave aberration with antiphase apodization has been obtained by one-dimensional pupil mask functions. In the presence of spherical aberration, suppression of optical side-lobes has increased on one side of the point spread function with the width of the periphery strips within the pupil mask. On introducing wave aberration effect, there exists dependence of the lateral resolution of central peak of the asymmetric point spread function on the amount of amplitude masking. However, the magnitude of intensity of central peak is originated be to amplified by the highest degree of amplitude and phase masking. Additionally, for aberrated asymmetric PSF, FWHM increases and it further decreases with the control parameters of amplitude and phase mask. The magnitude of this corollary can quantify the super resolution of diffracted structures under spherical aberration.

scholarly journals Spherical aberration correction in multiphoton fluorescence imaging using objective correction collar

2005 ◽  
Vol 10 (3) ◽  
pp. 034006 ◽  
Author(s):  
Wen Lo ◽  
Yen Sun ◽  
Sun-Jan Lin ◽  
Shiou-Hwa Jee ◽  
Chen-Yuan Dong
Keyword(s):  
Fluorescence Imaging ◽  
Spherical Aberration ◽  
Aberration Correction

Erratum: Spherical aberration correction in multiphoton fluorescence imaging using objective correction collar

2005 ◽  
Vol 10 (5) ◽  
pp. 059801
Author(s):  
Wen Lo ◽  
Yen Sun ◽  
Sun-Jan Lin ◽  
Shiou-Hwa Jee ◽  
Chen-Yuan Dong
Keyword(s):  
Fluorescence Imaging ◽  
Spherical Aberration ◽  
Aberration Correction

scholarly journals In vivo fluorescence imaging with a flat, lensless microscope

2020 ◽  
Author(s):  
Jesse K. Adams ◽  
Vivek Boominathan ◽  
Sibo Gao ◽  
Alex V. Rodriguez ◽  
Dong Yan ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Spatial Resolution ◽  
High Spatial Resolution ◽  
Animal Movement ◽  
Brain Activity ◽  
Phase Mask ◽  
Reconstruction Algorithms ◽  
Lensless Imaging ◽  
Computational Reconstruction

AbstractFluorescence imaging over large areas of the brain in freely behaving animals would allow researchers to better understand the relationship between brain activity and behavior; however, traditional microscopes capable of high spatial resolution and large fields of view (FOVs) require large and heavy lenses that restrict animal movement. While lensless imaging has the potential to achieve both high spatial resolution and large FOV with a thin lightweight device, lensless imaging has yet to be achieved in vivo due to two principal challenges: (a) biological tissue typically has lower contrast than resolution targets, and (b) illumination and filtering must be integrated into this non-traditional device architecture. Here, we show that in vivo fluorescence imaging is possible with a thin lensless microscope by optimizing the phase mask and computational reconstruction algorithms, and integrating fiber optic illumination and thin-film color filters. The result is a flat, lensless imager that achieves better than 10 μm spatial resolution and a FOV that is 30× larger than other cellular resolution miniature microscopes.

Optimized circularly symmetric phase mask to extend the depth of focus

2009 ◽  
Vol 26 (8) ◽  
pp. 1889 ◽  
Author(s):  
Feng Zhou ◽  
Ran Ye ◽  
Guangwei Li ◽  
Haitao Zhang ◽  
Dongsheng Wang
Keyword(s):  
Phase Mask ◽  
Depth Of Focus ◽  
Symmetric Phase
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