Reducing depth induced spherical aberration in 3D widefield fluorescence microscopy by wavefront coding using the SQUBIC phase mask

2014 ◽  
Author(s):  
Nurmohammed Patwary ◽  
Ana Doblas ◽  
Sharon V. King ◽  
Chrysanthe Preza
Keyword(s):  
Fluorescence Microscopy ◽  
Spherical Aberration ◽  
Phase Mask ◽  
Wavefront Coding

scholarly journals Coherent-hybrid STED: a tunable photo-physical pinhole for super-resolution imaging at high contrast

2018 ◽  
Author(s):  
António J. Pereira ◽  
Mafalda Sousa ◽  
Ana C. Almeida ◽  
Luísa T. Ferreira ◽  
Ana Rita Costa ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Spherical Aberration ◽  
Low Noise ◽  
Stimulated Emission ◽  
Lateral Resolution ◽  
Phase Mask ◽  
Background Suppression ◽  
High Contrast ◽  
Optical Sectioning ◽  
Neuron Cell Body

AbstractResolution in microscopy is not limited by diffraction as long as a nonlinear sample response is exploited. In a paradigmatic example, stimulated-emission depletion (STED) fluorescence microscopy fundamentally ‘breaks’ the diffraction limit by using a structured optical pattern to saturate depletion on a previously excited sample area. Two-dimensional (2D) STED, the canonical low-noise STED mode, structures the STED beam by using a vortex phase mask, achieving a significant lateral resolution improvement over confocal fluorescence microscopy. However, axial resolution and optical sectioning remain bound to diffraction. Here we use a tunable coherent-hybrid (CH) beam to improve optical sectioning, markedly reducing background fluorescence. CH-STED, which inherits the 2D-STED immunity to spherical aberration, diversifies the depletion strategy, allowing an optimal balance between two key metrics (lateral resolution and background suppression) to be found. CH-STED is used to perform high-contrast imaging of complex biological structures, such as the mitotic spindle and the neuron cell body.

Phase mask selection in wavefront coding systems: A design approach

2010 ◽  
Vol 48 (7-8) ◽  
pp. 779-785 ◽  
Author(s):  
Guillem Carles ◽  
Artur Carnicer ◽  
Salvador Bosch
Keyword(s):  
Design Approach ◽  
Phase Mask ◽  
Coding Systems ◽  
Wavefront Coding

scholarly journals Use of a spatial light modulator as an adaptable phase mask for wavefront coding

2010 ◽  
Vol 57 (10) ◽  
pp. 893-900 ◽  
Author(s):  
G. Carles ◽  
G. Muyo ◽  
S. Bosch ◽  
A.R. Harvey
Keyword(s):  
Spatial Light Modulator ◽  
Phase Mask ◽  
Light Modulator ◽  
Wavefront Coding

The analysis of ray aberration of arbitrary phase mask in the wavefront coding system

2008 ◽  
Vol 281 (18) ◽  
pp. 4577-4580 ◽  
Author(s):  
Weiwei Huang ◽  
Zi Ye ◽  
Wenzi Zhang ◽  
Tingyu Zhao ◽  
Feihong Yu
Keyword(s):  
Phase Mask ◽  
Coding System ◽  
Wavefront Coding ◽  
Arbitrary Phase

Analysis of wavefront coding imaging with cubic phase mask decenter and tilt

2016 ◽  
Vol 55 (25) ◽  
pp. 7009 ◽  
Author(s):  
Yijian Wu ◽  
Liquan Dong ◽  
Yuejin Zhao ◽  
Ming Liu ◽  
Xuhong Chu ◽  
...  
Keyword(s):  
Phase Mask ◽  
Cubic Phase ◽  
Wavefront Coding

scholarly journals A Wide-FoV Athermalized Infrared Imaging System with a Two-Piece Lens

2017 ◽  
Author(s):  
Bin Feng ◽  
Zelin Shi ◽  
Yaohong Zhao ◽  
Haizheng Liu ◽  
Li Liu
Keyword(s):  
Infrared Imaging ◽  
Imaging System ◽  
Operating Temperature ◽  
Transmission Efficiency ◽  
Phase Mask ◽  
Wide Field ◽  
Cubic Surface ◽  
Infrared Imaging System ◽  
Wavefront Coding ◽  
Wide Fov

For a wide field of view (FoV) wavefront coding athermalized infrared imaging system with a single decoding kernel, the off-axis aberration tends to cause artefacts. In order to correct off-axis aberration, many pieces of lenses will reduce the transmission efficiency and increase the weight and cost. To meet requirements for wide FoV, wide operating temperature and low weight of infrared imaging systems, this paper reports a wide-FoV wavefront coding athermalized infrared imaging system with a two-piece lens. Its principle, design, manufacture, measurement and performance validation are successively discussed. This paper constructs an optimization problem which maximizes the weighted mean of PSF consistency for both the FoV and operating temperature range. The two-piece lens contains four surfaces, where three aspheric surfaces are introduced to reduce optical off-axis aberrations and a cubic surface is introduced to achieve athermalization. The optical phase mask containing an aspheric surface and a cubic surface is manufactured by nano-metric machining of ion implanted material(NiIM). Experimental results validate that our wide-FoV wavefront coding athermalized infrared imaging system has a full FoV of 26.10° and an operating temperature over -20°C to +70°C.

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