In-vivo fluorescence detection of breast cancer growth factor receptors by fiber-optic probe

2018 ◽  
Author(s):  
Gilbert Bustamante ◽  
Bingzhi Wang ◽  
Lu-Zhe Sun ◽  
Jing Yong Ye ◽  
Frank DeLuna
Keyword(s):  
Breast Cancer ◽  
Growth Factor ◽  
Fluorescence Detection ◽  
Fiber Optic ◽  
Growth Factor Receptors ◽  
Cancer Growth ◽  
Fiber Optic Probe ◽  
Optic Probe ◽  
Breast Cancer Growth

scholarly journals Longitudinal Raman Spectroscopic Observation of Skin Biochemical Changes due to Chemotherapeutic Treatment for Breast Cancer in Small Animal Model

2017 ◽  
Vol 2017 ◽  
pp. 1-9
Author(s):  
Myeongsu Seong ◽  
NoSoung Myoung ◽  
Songhyun Lee ◽  
Hyeryun Jeong ◽  
Sang-Youp Yim ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Animal Model ◽  
Raman Spectra ◽  
Fiber Optic ◽  
Animal Experiments ◽  
Small Animal ◽  
Fiber Optic Probe ◽  
Small Animal Model ◽  
Biochemical Compositions ◽  
Optic Probe

The cancer field effect (CFE) has been highlighted as one of indirect indications for tissue variations that are insensitive to conventional diagnostic techniques. In this research, we had a hypothesis that chemotherapy for breast cancer would affect skin biochemical compositions that would be reflected by Raman spectral changes. We used a fiber-optic probe-based Raman spectroscopy to perform preliminary animal experiments to validate the hypothesis. Firstly, we verified the probing depth of the fiber-optic probe (~800 μm) using a simple intravenous fat emulsion-filled phantom having a silicon wafer at the bottom inside a cuvette. Then, we obtained Raman spectra during breast cancer treatment by chemotherapy from a small animal model in longitudinal manner. Our results showed that the treatment causes variations of biochemical compositions in the skin. For further validation, the Raman spectra will have to be collected from more populations and spectra will need to be compared with immunohistochemistry of the breast tissue.

Glipizide Combined with ANP Suppresses Breast Cancer Growth and Metastasis by Inhibiting Angiogenesis through VEGF/VEGFR2 Signaling

2021 ◽  
Vol 21 ◽  
Author(s):  
Guanquan Mao ◽  
Shuting Zheng ◽  
Jinlian Li ◽  
Xiaohua Liu ◽  
Qin Zhou ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Natriuretic Peptide ◽  
Umbilical Vein ◽  
Tube Formation ◽  
Cancer Growth ◽  
Vascular Endothelial ◽  
Breast Cancer Growth ◽  
Endothelial Growth Factor

Background: Breast cancer is one of the most common cancers worldwide among women, and angiogenesis has an important effect on its growth and metastasis. Glipizide, which is a widely used drug for type 2 diabetes mellitus, has been reported to inhibit tumor growth and metastasis by upregulating the expression of natriuretic peptide receptor A (NPRA). Atrial natriuretic peptide (ANP), the receptor of NPRA, plays an important role in angiogenesis. The purpose of this study was to explore the effect of glipizide combined with ANP on breast cancer growth and metastasis. Methods: To investigate the effect of glipizide combined with ANP on breast cancer, glipizide, ANP or glipizide combined with ANP was intraperitoneally injected into MMTV-PyMT mice. To explore whether the anticancer efficacy of glipizide combined with ANP was correlated with angiogenesis, a tube formation assay was performed. Results: Glipizide combined with ANP was found to inhibit breast cancer growth and metastasis in MMTV-PyMT mice, which spontaneously develop breast cancer. Furthermore, the inhibitory effect of ANP combined with glipizide was better than that of glipizide alone. ANP combined with glipizide significantly inhibited tube formation of human umbilical vein endothelial cells (HUVECs) by suppressing vascular endothelial growth factor (VEGF)/VEGFR2 (vascular endothelial growth factor receptor 2) signaling. Conclusions: These results demonstrate that glipizide combined with ANP has a greater potential than glipizide alone to be repurposed as effective agents for the treatment of breast cancer by targeting tumor-induced angiogenesis.

scholarly journals IL-1 drives breast cancer growth and bone metastasis in vivo

Oncotarget ◽  
2016 ◽  
Vol 7 (46) ◽  
pp. 75571-75584 ◽  
Author(s):  
Ingunn Holen ◽  
Diane V. Lefley ◽  
Sheila E. Francis ◽  
Sarah Rennicks ◽  
Steven Bradbury ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Bone Metastasis ◽  
Cancer Growth ◽  
Breast Cancer Growth

scholarly journals Estrogen Deprivation and Inhibition of Breast Cancer Growth in Vivo through Activation of the Orphan Nuclear Receptor Liver X Receptor

2007 ◽  
Vol 21 (8) ◽  
pp. 1781-1790 ◽  
Author(s):  
Haibiao Gong ◽  
Ping Guo ◽  
Yonggong Zhai ◽  
Jie Zhou ◽  
Hirdesh Uppal ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Liver X Receptor ◽  
Cancer Growth ◽  
Estrogen Metabolism ◽  
Endocrine Disorders ◽  
Estrogen Deprivation ◽  
Uterine Epithelial Cell ◽  
Hepatic Expression ◽  
Breast Cancer Growth

Abstract Estrogen plays an important role in normal physiology. It is also a risk factor for breast cancer, and antiestrogen therapies have been shown to be effective in the treatment and prevention of breast cancers. The liver is important for estrogen metabolism, and a compromised liver function has been linked to hyperestrogenism in patients. In this report, we showed that the liver X receptor (LXR) controls estrogen homeostasis by regulating the basal and inducible hepatic expression of estrogen sulfotransferase (Est, or Sult1e1), an enzyme critical for metabolic estrogen deactivation. Genetic or pharmacological activation of LXR resulted in Est induction, which in turn inhibited estrogen-dependent uterine epithelial cell proliferation and gene expression, as well as breast cancer growth in a nude mouse model of tumorigenicity. We further established that Est is a transcriptional target of LXR, and deletion of the Est gene in mice abolished the LXR effect on estrogen deprivation. Interestingly, Est regulation by LXR appeared to be liver specific, further underscoring the role of liver in estrogen metabolism. Activation of LXR failed to induce other major estrogen-metabolizing enzymes, suggesting that the LXR effect on estrogen metabolism is Est specific. In summary, our results have revealed a novel mechanism controlling estrogen homeostasis in vivo and may have implications for drug development in the treatment of breast cancer and other estrogen-related cancerous endocrine disorders.

scholarly journals Small molecule STAT3 inhibitor, 6Br-6a suppresses breast cancer growth in vitro and in vivo

2020 ◽  
Vol 121 ◽  
pp. 109502 ◽  
Author(s):  
Zhe Liu ◽  
Xianmin Ge ◽  
Yuchen Gu ◽  
Yingying Huang ◽  
Hao Liu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Small Molecule ◽  
Cancer Growth ◽  
Breast Cancer Growth

A Chemometric Analysis for Evaluation of Early-Stage Cartilage Degradation by Infrared Fiber-Optic Probe Spectroscopy

2005 ◽  
Vol 59 (12) ◽  
pp. 1527-1533 ◽  
Author(s):  
Guiyang Li ◽  
Mary Thomson ◽  
Edward Dicarlo ◽  
Xu Yang ◽  
Bryan Nestor ◽  
...  
Keyword(s):  
Fiber Optic ◽  
Early Stage ◽  
Cartilage Degradation ◽  
Fiber Optic Probe ◽  
Replacement Surgery ◽  
Multiple Regions ◽  
Grading Scale ◽  
Optic Probe ◽  
Probe Spectroscopy

In vivo identification of early-stage cartilage degradation could positively impact disease progression in osteoarthritis, but to date remains a challenge. The primary goal of this study was to develop an infrared fiber-optic probe (IFOP) chemometric method using partial least squares (PLS1) to objectively determine the degree of cartilage degradation. Arthritic human tibial plateaus ( N = 61) were obtained during knee replacement surgery and analyzed by IFOP. IFOP data were collected from multiple regions of each specimen and the cartilage graded according to the Collins Visual Grading Scale of 0, 1, 2, or 3. These grades correspond to cartilage morphology that displayed normal, swelling or softening, superficially slight fibrillation, and deeper fibrillation or serious fibrillation, respectively. The model focused on detecting early cartilage degradation and therefore utilized data from grades 0, 1, and 2. The best PLS1 calibration utilized the spectral range 1733–984 cm−1, and independent validation of the model utilizing 206 spectra to create a model and 105 independent test spectra resulted in a correlation between the predicted and actual Collins grade of R2 = 0.8228 with a standard error of prediction of 0.258 with a PLS1 rank of 15 PLS factors. A preliminary PLS1 calibration that utilized a cross-validation technique to investigate the possibility of correlation with histological tissue grade (33 spectra from 18 tissues) resulted in R2 = 0.8408 using only eight PLS factors, a very encouraging outcome. Thus, the groundwork for use of IFOP-based chemometric determination of early cartilage degradation has been established.

Photodynamic acidification therapy to reduce triple negative breast cancer growth in vivo.

2016 ◽  
Vol 34 (15_suppl) ◽  
pp. e12574-e12574 ◽  
Author(s):  
Nuha Buchanan Kadri ◽  
Matthew Gdovin ◽  
Nizar Alyassin ◽  
Justin Avila ◽  
Aryana Cruz ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Cancer Growth ◽  
Breast Cancer Growth
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