Label-free, multi-contrast optical coherence tomography for study of skin melanoma mice in vivo

2018 ◽  
Author(s):  
Ting-Han Lin ◽  
Chung-Hsing Chang ◽  
Ya-Shuan Chou ◽  
Wen-Chuan Kuo ◽  
Pei-Yu Lai
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence ◽  
Label Free ◽  
Skin Melanoma

scholarly journals In vivo label-free measurement of lymph flow velocity and volumetric flow rates using Doppler optical coherence tomography

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Cedric Blatter ◽  
Eelco F. J. Meijer ◽  
Ahhyun S. Nam ◽  
Dennis Jones ◽  
Brett E. Bouma ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Flow Velocity ◽  
Lymph Flow ◽  
Optical Coherence ◽  
Label Free ◽  
Flow Rates ◽  
Doppler Optical Coherence Tomography

scholarly journals In vivo label-free lymphangiography of cutaneous lymphatic vessels in human burn scars using optical coherence tomography

2016 ◽  
Vol 7 (12) ◽  
pp. 4886 ◽  
Author(s):  
Peijun Gong ◽  
Shaghayegh Es’haghian ◽  
Karl-Anton Harms ◽  
Alexandra Murray ◽  
Suzanne Rea ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Lymphatic Vessels ◽  
Optical Coherence ◽  
Label Free

scholarly journals In vivo, label-free, three-dimensional quantitative imaging of kidney microcirculation using Doppler optical coherence tomography

2011 ◽  
Vol 91 (11) ◽  
pp. 1596-1604 ◽  
Author(s):  
Jeremiah Wierwille ◽  
Peter M Andrews ◽  
Maristela L Onozato ◽  
James Jiang ◽  
Alex Cable ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Quantitative Imaging ◽  
Three Dimensional ◽  
Optical Coherence ◽  
Label Free ◽  
Doppler Optical Coherence Tomography

scholarly journals Label-Free In Vivo Imaging of Corneal Lymphatic Vessels Using Microscopic Optical Coherence Tomography

2017 ◽  
Vol 58 (13) ◽  
pp. 5880 ◽  
Author(s):  
Jens Horstmann ◽  
Hinnerk Schulz-Hildebrandt ◽  
Felix Bock ◽  
Sebastian Siebelmann ◽  
Eva Lankenau ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
In Vivo Imaging ◽  
Lymphatic Vessels ◽  
Optical Coherence ◽  
Label Free

scholarly journals OCT2Hist: Non-Invasive Virtual Biopsy Using Optical Coherence Tomography

2021 ◽  
Author(s):  
Yonatan Winetraub ◽  
Edwin Yuan ◽  
Itamar Terem ◽  
Caroline Yu ◽  
Warren Chan ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Ground Truth ◽  
Disease Diagnosis ◽  
Optical Coherence ◽  
Label Free ◽  
Generative Adversarial Network ◽  
Non Invasive ◽  
Adversarial Network ◽  
Trained Neural Network

Histological haematoxylin and eosin–stained (H&E) tissue sections are used as the gold standard for pathologic detection of cancer, tumour margin detection, and disease diagnosis1. Producing H&E sections, however, is invasive and time-consuming. Non-invasive optical imaging modalities, such as optical coherence tomography (OCT), permit label-free, micron-scale 3D imaging of biological tissue microstructure with significant depth (up to 1mm) and large fields-of-view2, but are difficult to interpret and correlate with clinical ground truth without specialized training3. Here we introduce the concept of a virtual biopsy, using generative neural networks to synthesize virtual H&E sections from OCT images. To do so we have developed a novel technique, “optical barcoding”, which has allowed us to repeatedly extract the 2D OCT slice from a 3D OCT volume that corresponds to a given H&E tissue section, with very high alignment precision down to 25 microns. Using 1,005 prospectively collected human skin sections from Mohs surgery operations of 71 patients, we constructed the largest dataset of H&E images and their corresponding precisely aligned OCT images, and trained a conditional generative adversarial network4 on these image pairs. Our results demonstrate the ability to use OCT images to generate high-fidelity virtual H&E sections and entire 3D H&E volumes. Applying this trained neural network to in vivo OCT images should enable physicians to readily incorporate OCT imaging into their clinical practice, reducing the number of unnecessary biopsy procedures.

258: Diagnosis and Intraoperative Staging of Bladder Disease by in-vivo Optical Coherence Tomography (OCT)

2004 ◽  
Vol 171 (4S) ◽  
pp. 68-68 ◽  
Author(s):  
Markus D. Sachs ◽  
Dmitry Daniltchenko ◽  
Eva Lankenau ◽  
Frank Koenig ◽  
Gerion Huettmann ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence ◽  
Bladder Disease

scholarly journals Evaluating Retinal and Choroidal Perfusion Changes after Isometric and Dynamic Activity Using Optical Coherence Tomography Angiography

Diagnostics ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 808
Author(s):  
Max Philipp Brinkmann ◽  
Nikolas Xavier Kibele ◽  
Michelle Prasuhn ◽  
Vinodh Kakkassery ◽  
Mario Damiano Toro ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence Tomography Angiography ◽  
Isometric Exercise ◽  
Dynamic Exercise ◽  
Exercise Heart Rate ◽  
Optical Coherence ◽  
Dynamic Activity ◽  
Capillary Plexus ◽  
Choroidal Perfusion

Optical coherence tomography angiography (OCTA) is a non-invasive tool for imaging and quantifying the retinal and choroidal perfusion state in vivo. This study aimed to evaluate the acute effects of isometric and dynamic exercise on retinal and choroidal sublayer perfusion using OCTA. A pilot study was conducted on young, healthy participants, each of whom performed a specific isometric exercise on the first day and a dynamic exercise the day after. At baseline and immediately after the exercise, heart rate (HR), mean arterial pressure (MAP), superficial capillary plexus perfusion (SCPP), deep capillary plexus perfusion (DCPP), choriocapillaris perfusion (CCP), Sattlers’s layer perfusion (SLP), and Haller’s layer perfusion (HLP) were recorded. A total of 34 eyes of 34 subjects with a mean age of 32.35 ± 7.87 years were included. HR as well as MAP increased significantly after both types of exercise. Both SCPP and DCPP did not show any significant alteration due to isometric or dynamic exercise. After performing dynamic exercise, CCP, SLP, as well as HLP significantly increased. Changes in MAP correlated significantly with changes in HLP after the dynamic activity. OCTA-based analysis in healthy adults following physical activity demonstrated a constant retinal perfusion, supporting the theory of autoregulatory mechanisms. Dynamic exercise, as opposed to isometric activity, significantly changed choroidal perfusion. OCTA imaging may represent a novel and sensitive tool to expand the diagnostic spectrum in the field of sports medicine.

scholarly journals Quantitative Swept-Source Optical Coherence Tomography of Early Enamel Erosion in vivo

2017 ◽  
Vol 51 (4) ◽  
pp. 410-418 ◽  
Author(s):  
Rupert S. Austin ◽  
Maisalamah Haji Taha ◽  
Frederic Festy ◽  
Richard Cook ◽  
Manoharan Andiappan ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Healthy Volunteers ◽  
Orange Juice ◽  
Signal Intensity ◽  
Surface States ◽  
Enamel Surface ◽  
Optical Coherence ◽  
Swept Source ◽  
Water Rinsing

Swept-source optical coherence tomography (SS-OCT) shows potential for the in vivo quantitative evaluation of micro-structural enamel surface phenomena occurring during early erosive demineralization. This randomized controlled single-blind cross-over clinical study aimed to evaluate the use of SS-OCT for detecting optical changes in the enamel of 30 healthy volunteers subjected to orange juice rinsing (erosive challenge) in comparison to mineral water rinsing (control), according to wiped and non-wiped enamel surface states. Participants were randomly allocated to 60 min of orange juice rinsing (pH 3.8) followed by 60 min of water rinsing (pH 6.7) and vice versa, with a 2-week wash-out period. In addition, the labial surfaces of the right or left maxillary incisors were wiped prior to SS-OCT imaging. An automated ImageJ algorithm was designed to analyse the back-scattered OCT signal intensity (D) after orange juice rinsing compared to after water rinsing. D was quantified as the OCT signal scattering from the 33 µm sub-surface enamel, normalised by the total OCT signal intensity entering the enamel. The back-scattered OCT signal intensity increased by 3.1% (95% CI 1.1-5.1%) in the wiped incisors and by 3.5% (95% CI 1.5-5.5%) in the unwiped incisors (p < 0.0001). Wiping reduced the back-scattered OCT signal intensity by 1.7% (95% CI -3.2 to -0.3%; p = 0.02) in comparison to the unwiped enamel surfaces for both rinsing solutions (p = 0.2). SS-OCT detected OCT signal changes in the superficial sub-surface enamel of maxillary central incisor teeth of healthy volunteers after orange juice rinsing.

Sign in / Sign up

Export Citation Format

Share Document