Combined fluorescence lifetime imaging-optical coherence tomography for in vivo label-free assessment of high-risk atherosclerotic plaque

2019 ◽  
Author(s):  
Hyeong Soo Nam ◽  
Sunwon Kim ◽  
Woo Jae Kang ◽  
Min Woo Lee ◽  
Jeongmoo Han ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
High Risk ◽  
Atherosclerotic Plaque ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
Optical Coherence ◽  
Label Free ◽  
Lifetime Imaging ◽  
Imaging Optical Coherence Tomography

scholarly journals Endoscopic Fluorescence Lifetime Imaging for In Vivo Intraoperative Diagnosis of Oral Carcinoma

2013 ◽  
Vol 19 (4) ◽  
pp. 791-798 ◽  
Author(s):  
Yinghua Sun ◽  
Jennifer E. Phipps ◽  
Jeremy Meier ◽  
Nisa Hatami ◽  
Brian Poirier ◽  
...  
Keyword(s):  
Head And Neck ◽  
Fluorescence Lifetime ◽  
Imaging System ◽  
Spectral Band ◽  
Average Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
Label Free ◽  
Lifetime Imaging ◽  
Surgical Field

AbstractA clinically compatible fluorescence lifetime imaging microscopy (FLIM) system was developed. The system was applied to intraoperative in vivo imaging of head and neck squamous cell carcinoma (HNSCC). The endoscopic FLIM prototype integrates a gated (down to 0.2 ns) intensifier imaging system and a fiber-bundle endoscope (0.5-mm-diameter, 10,000 fibers with a gradient index lens objective 0.5 NA, 4-mm field of view), which provides intraoperative access to the surgical field. Tissue autofluorescence was induced by a pulsed laser (337 nm, 700 ps pulse width) and collected in the 460 ± 25 nm spectral band. FLIM experiments were conducted at 26 anatomic sites in ten patients during head and neck cancer surgery. HNSCC exhibited a weaker florescence intensity (~50% less) when compared with healthy tissue and a shorter average lifetime (τHNSCC = 1.21 ± 0.04 ns) than the surrounding normal tissue (τN = 1.49 ± 0.06 ns). This work demonstrates the potential of FLIM for label-free head and neck tumor demarcation during intraoperative surgical procedures.

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