Compact imaging system for quantitative fluorescence sensing through autofluorescent, scattering and absorbing media (Conference Presentation)

2018 ◽  
Author(s):  
Zoltán S. Göröcs ◽  
Yair Rivenson ◽  
Hatice Ceylan Koydemir ◽  
Derek Tseng ◽  
Tamara L. Troy ◽  
...  
Keyword(s):  
Imaging System ◽  
Fluorescence Sensing ◽  
Absorbing Media ◽  
Quantitative Fluorescence

Quantitative Fluorescence Sensing Through Highly Autofluorescent, Scattering, and Absorbing Media Using Mobile Microscopy

ACS Nano ◽  
2016 ◽  
Vol 10 (9) ◽  
pp. 8989-8999 ◽  
Author(s):  
Zoltán Göröcs ◽  
Yair Rivenson ◽  
Hatice Ceylan Koydemir ◽  
Derek Tseng ◽  
Tamara L. Troy ◽  
...  
Keyword(s):  
Fluorescence Sensing ◽  
Absorbing Media ◽  
Quantitative Fluorescence

scholarly journals AIE-active bis-cyanostilbene-based organogels for quantitative fluorescence sensing of CO2 based on molecular recognition principles

2018 ◽  
Vol 6 (34) ◽  
pp. 9232-9237 ◽  
Author(s):  
Yao Ma ◽  
Massimo Cametti ◽  
Zoran Džolić ◽  
Shimei Jiang
Keyword(s):  
Detection Limit ◽  
Molecular Recognition ◽  
Fluorescence Sensing ◽  
Quantitative Fluorescence

Fluorescence sensing of CO2 is achieved by the use of gel aggregates and xerogel systems made with the aggregation induced emissive bis-cyanostilbene derivative 1 reaching a detection limit as low as 4.5 ppm.

Mobile Microscope for Quantitative Fluorescence Sensing Through Highly Autofluorescent and Scattering Media

2017 ◽  
Author(s):  
Zoltán Göröcs ◽  
Yair Rivenson ◽  
Hatice Ceylan Koydemir ◽  
Derek Tseng ◽  
Tamara L. Troy ◽  
...  
Keyword(s):  
Scattering Media ◽  
Fluorescence Sensing ◽  
Quantitative Fluorescence

scholarly journals Accuracy and precision in quantitative fluorescence microscopy

2009 ◽  
Vol 185 (7) ◽  
pp. 1135-1148 ◽  
Author(s):  
Jennifer C. Waters
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Imaging System ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Digital Cameras ◽  
Accuracy And Precision ◽  
Fluorescent Molecules ◽  
Quantitative Fluorescence ◽  
Biology Research

The light microscope has long been used to document the localization of fluorescent molecules in cell biology research. With advances in digital cameras and the discovery and development of genetically encoded fluorophores, there has been a huge increase in the use of fluorescence microscopy to quantify spatial and temporal measurements of fluorescent molecules in biological specimens. Whether simply comparing the relative intensities of two fluorescent specimens, or using advanced techniques like Förster resonance energy transfer (FRET) or fluorescence recovery after photobleaching (FRAP), quantitation of fluorescence requires a thorough understanding of the limitations of and proper use of the different components of the imaging system. Here, I focus on the parameters of digital image acquisition that affect the accuracy and precision of quantitative fluorescence microscopy measurements.

79 THE AMOUNT OF TELOMERIC DNA IN CLONED CATTLE AND THEIR CALVES IS LESS THAN THAT OF AGE MATCHED CATTLE

2006 ◽  
Vol 18 (2) ◽  
pp. 148
Author(s):  
B. C. Yang ◽  
G. S. Im ◽  
Y. H. Kim ◽  
J. W. Choi ◽  
Y. S. Park ◽  
...  
Keyword(s):  
Tandem Repeats ◽  
Imaging System ◽  
Skin Fibroblasts ◽  
Telomeric Dna ◽  
Eukaryotic Chromosome ◽  
Dna Repeat ◽  
Fetal Fibroblasts ◽  
And Control ◽  
Quantitative Fluorescence

A telomere is a structure consisting of tandem repeats sequences of (TTAGGG)n at the end of the eukaryotic chromosome. Telomere lengths in animals vary by species, age, and tissues, as well as environment. This experiment concentrated on the amount of telomeric DNA in cloned cattle, their calves, and age-matched normal cattle. Using somatic cell nuclear transfer (SCNT), we had obtained 16 cloned Korean Native cows derived from ear skin fibroblasts and two cloned bulls from fetal fibroblasts. In addition, four female calves were produced from each cloned cow by artificial insemination. Control cattle selected to have matched age and the same raising place served as counter-parts of cloned the cattle in this study. The lymphocytes of all cloned cattle, their calves, and the age-matched controls were examined for telomere quantity. The amount of telomeric DNA was analyzed by quantitative fluorescence after in situ hybridization (Q-FISH) with a human telomeric DNA repeat probe. A minimum of 100 interphase nuclei from each set of harvests was studied to determine the mean and medium percentages of telomeric DNA using the MetaMorph Imaging System (Universal Imaging Co., West Chester, PA, USA). The amount of telomeric DNA obtained was found to decrease in cloned and control animals during growth. The amounts of telomeric DNA in cloned cattle from both ear skin fibroblasts (n = 16) and fetal fibroblasts (n = 2) was less than that of age-matched controls (P < 0.01). Surprisingly, the amount of telomeric DNA of calves from cloned cattle was also lower than that of age matched controls (n = 4, P < 0.01). The results showed a remarkable difference in the amount of telomeric DNA between SCNT cloned cattle and normal cattle. In conclusion, the telomeres of cloned animal and their calves are significantly shorter than those of normal cattle. Moreover, the short telomeres in calves could be inherited from their cloned mothers.

A novel camera phone-based platform for quantitative fluorescence sensing

2013 ◽  
Vol 5 (8) ◽  
pp. 1904 ◽  
Author(s):  
Stephen O'Driscoll ◽  
Brian D. MacCraith ◽  
Conor S. Burke
Keyword(s):  
Fluorescence Sensing ◽  
Camera Phone ◽  
Quantitative Fluorescence

An inexpensive, easy to use video imaging system for intracellular Ca2+ and quantitative fluorescence microscopy

1993 ◽  
Vol 51 ◽  
pp. 620-621
Author(s):  
Eric Gruenstein ◽  
Jesus Luna
Keyword(s):  
Fluorescence Microscopy ◽  
Calcium Imaging ◽  
Imaging System ◽  
Cellular Responses ◽  
Free Calcium ◽  
Video Imaging ◽  
High Degree ◽  
Kinetics Of ◽  
Quantitative Fluorescence ◽  
Stimulation Of

Fura-2 is the most commonly used member of a family of calcium sensitive flourescent dyes that allows the measurement of intracellular free calcium (Cai by dual excitation fluorimetry. The use of this dye in conjunction with video imaging microscopy permits visualization of changes in Cai with a high degree of spatial resolution. This resolution in turn allows the detection of Cai gradients, waves, and other localized cellular responses not easily detected by photometric techniques. Despite these advantages, most published reports have utilized photometry rather than imaging, due largely to the higher cost and greater complexity of use of the latter technique. With these problems in mind, we have developed a turnkey calcium imaging system that is both inexpensive and simple enough to use that it can be mastered in 2-3 hours.Data showing the distribution of free Ca2+ in the cytoplasm of a quiescent human fibroblast and the kinetics of stimulation of these cells with mitogens are illustrated on the next page.

Sign in / Sign up

Export Citation Format

Share Document