Super-resolution localization microscopy with large field-of-view for mapping synaptic connectivity at multiple scales (Conference Presentation)

2018 ◽  
Author(s):  
Zhenli Huang ◽  
Bo Xin ◽  
Yujie Wang ◽  
Luchang Li ◽  
Zhaoning Zhang ◽  
...  
Keyword(s):  
Multiple Scales ◽  
Super Resolution ◽  
Field Of View ◽  
Synaptic Connectivity ◽  
Large Field ◽  
Localization Microscopy

scholarly journals High-power homogeneous illumination for super-resolution localization microscopy with large field-of-view

2017 ◽  
Vol 25 (12) ◽  
pp. 13382 ◽  
Author(s):  
Zeyu Zhao ◽  
Bo Xin ◽  
Luchang Li ◽  
Zhen-Li Huang
Keyword(s):  
High Power ◽  
Super Resolution ◽  
Field Of View ◽  
Large Field ◽  
Localization Microscopy

Large Field-of-View Super-Resolution Optical Microscopy Based on Planar Polymer Waveguides

ACS Photonics ◽  
2021 ◽  
Author(s):  
Anders Kokkvoll Engdahl ◽  
Stefan Belle ◽  
Tung-Cheng Wang ◽  
Ralf Hellmann ◽  
Thomas Huser ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Super Resolution ◽  
Field Of View ◽  
Large Field ◽  
Polymer Waveguides

scholarly journals Large field-of-view super-resolution image obtained by manipulating submerged microsphere

2018 ◽  
Vol 1074 ◽  
pp. 012172
Author(s):  
Shilin Gao ◽  
Kai Meng ◽  
Zhan Yang ◽  
Huicong Liu ◽  
Tao Chen ◽  
...  
Keyword(s):  
Super Resolution ◽  
Field Of View ◽  
Large Field ◽  
Resolution Image

scholarly journals Hessian single molecule localization microscopy using sCMOS camera

2018 ◽  
Author(s):  
Fudong Xue ◽  
Wenting He ◽  
Fan Xu ◽  
Mingshu Zhang ◽  
Liangyi Chen ◽  
...  
Keyword(s):  
Single Molecule ◽  
Signal To Noise Ratio ◽  
Imaging Techniques ◽  
Super Resolution ◽  
Fast Imaging ◽  
Large Field ◽  
Localization Microscopy ◽  
Readout Noise ◽  
Single Pixel ◽  
Single Molecule Localization Microscopy

AbstractSingle-molecule localization microscopy (SMLM) has the highest spatial resolution among the existing super-resolution (SR) imaging techniques, but its temporal resolution needs further improvement. An sCMOS camera can effectively increase the imaging rate due to its large field of view and fast imaging speed. Using an sCMOS camera for SMLM imaging can significantly improve the imaging time resolution, but the unique single pixel-dependent readout noise of sCMOS cameras severely limits their application in SMLM imaging. This paper develops a Hessian-based SMLM (Hessian-SMLM) method that can correct the variance, gain and offset of a single pixel of a camera and effectively eliminate the pixel-dependent readout noise of sCMOS cameras, especially when the signal-to-noise ratio is low. Using Hessian SMLM to image mEos3.2-labeled actin was able to significantly reduce the artifacts due to camera noise.

Design of Super-Resolution Telescopic Imaging Optical System with Relative Large Field-of-View

2020 ◽  
Vol 40 (13) ◽  
pp. 1322002
Author(s):  
王超 Wang Chao ◽  
史浩东 Shi Haodong ◽  
李英超 Li Yingchao ◽  
付强 Fu Qiang ◽  
刘壮 Liu Zhuang
Keyword(s):  
Optical System ◽  
Super Resolution ◽  
Field Of View ◽  
Large Field
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