In vivo study of lipid synthesis and lipolysis dynamics by stimulated Raman scattering microscopy

2018 ◽  
Author(s):  
Xuesong Li ◽  
Yan Li ◽  
Sicong He ◽  
Congping Chen ◽  
Zhongya Qin ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Lipid Synthesis ◽  
In Vivo Study ◽  
Stimulated Raman

scholarly journals Video-Rate Molecular Imaging in Vivo with Stimulated Raman Scattering

Science ◽  
2010 ◽  
Vol 330 (6009) ◽  
pp. 1368-1370 ◽  
Author(s):  
B. G. Saar ◽  
C. W. Freudiger ◽  
J. Reichman ◽  
C. M. Stanley ◽  
G. R. Holtom ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Stimulated Raman

scholarly journals Spectrometer-free vibrational imaging by retrieving stimulated Raman signal from highly scattered photons

2015 ◽  
Vol 1 (9) ◽  
pp. e1500738 ◽  
Author(s):  
Chien-Sheng Liao ◽  
Pu Wang ◽  
Ping Wang ◽  
Junjie Li ◽  
Hyeon Jeong Lee ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Collection Efficiency ◽  
Mouse Skin ◽  
Spectroscopic Imaging ◽  
Acquisition Time ◽  
Raman Signal ◽  
Stimulated Raman ◽  
Photon Collection

In vivo vibrational spectroscopic imaging is inhibited by relatively slow spectral acquisition on the second scale and low photon collection efficiency for a highly scattering system. Recently developed multiplex coherent anti-Stokes Raman scattering and stimulated Raman scattering techniques have improved the spectral acquisition time down to microsecond scale. These methods using a spectrometer setting are not suitable for turbid systems in which nearly all photons are scattered. We demonstrate vibrational imaging by spatial frequency multiplexing of incident photons and single photodiode detection of a stimulated Raman spectrum within 60 μs. Compared to the spectrometer setting, our method improved the photon collection efficiency by two orders of magnitude for highly scattering specimens. We demonstrated in vivo imaging of vitamin E distribution on mouse skin and in situ imaging of human breast cancerous tissues. The reported work opens new opportunities for spectroscopic imaging in a surgical room and for development of deep-tissue Raman spectroscopy toward molecular level diagnosis.

scholarly journals Live-Cell Imaging and Quantification of PolyQ Aggregates by Stimulated Raman Scattering of Selective Deuterium Labeling

2019 ◽  
Author(s):  
Kun Miao ◽  
Lu Wei
Keyword(s):  
Raman Scattering ◽  
Live Cell Imaging ◽  
Neurodegenerative Disorder ◽  
Stimulated Raman Scattering ◽  
Live Cells ◽  
Deuterium Labeling ◽  
Multicellular Organisms ◽  
Subcellular Resolution ◽  
Stimulated Raman

AbstractHuntington’s disease, a major neurodegenerative disorder, involves deposition of aggregation-prone proteins with long polyglutamine (polyQ) expansions. The ability to non-perturbatively visualize the formation of aggregates could offer new molecular insight for their pathologic roles. Here, we propose stimulated Raman scattering imaging of deuterium-labeled glutamine to investigate native polyQ aggregates in live cells with subcellular resolution. Through the enrichment of deuterated glutamine in the polyQ sequence of mutant Huntingtin (mHtt) proteins, we first achieved sensitive and specific SRS imaging of carbon-deuterium bonds (C-D) from aggregates without GFP labeling. These aggregates become 1.8-fold denser compared to those with GFP. Second, we performed ratiometric quantification, which revealed a dependence of protein compositions on aggregation sizes. Moreover, we calculated the absolute concentrations for sequestered mHtt and non-mHtt proteins within the same aggregates. Our method may readily reveal new features of polyQ aggregates and could be suited for in vivo investigations on multicellular organisms.

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