Quantitative Imaging of Lipid Synthesis and Lipolysis Dynamics in Caenorhabditis elegans by Stimulated Raman Scattering Microscopy

2018 ◽  
Vol 91 (3) ◽  
pp. 2279-2287 ◽  
Author(s):  
Xuesong Li ◽  
Yan Li ◽  
Meijuan Jiang ◽  
Wanjie Wu ◽  
Sicong He ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Quantitative Imaging ◽  
Lipid Synthesis ◽  
Stimulated Raman

scholarly journals Label-Free Quantitative Imaging of Cholesterol in Intact Tissues by Hyperspectral Stimulated Raman Scattering Microscopy

2013 ◽  
Vol 125 (49) ◽  
pp. 13280-13284 ◽  
Author(s):  
Ping Wang ◽  
Junjie Li ◽  
Pu Wang ◽  
Chun-Rui Hu ◽  
Delong Zhang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Quantitative Imaging ◽  
Label Free ◽  
Stimulated Raman

scholarly journals Live-Cell Quantitative Imaging of Proteome Degradation by Stimulated Raman Scattering

2014 ◽  
Vol 53 (22) ◽  
pp. 5596-5599 ◽  
Author(s):  
Yihui Shen ◽  
Fang Xu ◽  
Lu Wei ◽  
Fanghao Hu ◽  
Wei Min
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Quantitative Imaging ◽  
Live Cell ◽  
Stimulated Raman

scholarly journals Label-Free Quantitative Imaging of Cholesterol in Intact Tissues by Hyperspectral Stimulated Raman Scattering Microscopy

2013 ◽  
Vol 52 (49) ◽  
pp. 13042-13046 ◽  
Author(s):  
Ping Wang ◽  
Junjie Li ◽  
Pu Wang ◽  
Chun-Rui Hu ◽  
Delong Zhang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Quantitative Imaging ◽  
Label Free ◽  
Stimulated Raman

scholarly journals Volumetric chemical imaging by clearing-enhanced stimulated Raman scattering microscopy

2019 ◽  
Vol 116 (14) ◽  
pp. 6608-6617 ◽  
Author(s):  
Mian Wei ◽  
Lingyan Shi ◽  
Yihui Shen ◽  
Zhilun Zhao ◽  
Asja Guzman ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Optical Microscopy ◽  
Stimulated Raman Scattering ◽  
Three Dimensional ◽  
Lipid Synthesis ◽  
Chemical Imaging ◽  
Label Free ◽  
Tissue Clearing ◽  
Metabolic Activities ◽  
Stimulated Raman

Three-dimensional visualization of tissue structures using optical microscopy facilitates the understanding of biological functions. However, optical microscopy is limited in tissue penetration due to severe light scattering. Recently, a series of tissue-clearing techniques have emerged to allow significant depth-extension for fluorescence imaging. Inspired by these advances, we develop a volumetric chemical imaging technique that couples Raman-tailored tissue-clearing with stimulated Raman scattering (SRS) microscopy. Compared with the standard SRS, the clearing-enhanced SRS achieves greater than 10-times depth increase. Based on the extracted spatial distribution of proteins and lipids, our method reveals intricate 3D organizations of tumor spheroids, mouse brain tissues, and tumor xenografts. We further develop volumetric phasor analysis of multispectral SRS images for chemically specific clustering and segmentation in 3D. Moreover, going beyond the conventional label-free paradigm, we demonstrate metabolic volumetric chemical imaging, which allows us to simultaneously map out metabolic activities of protein and lipid synthesis in glioblastoma. Together, these results support volumetric chemical imaging as a valuable tool for elucidating comprehensive 3D structures, compositions, and functions in diverse biological contexts, complementing the prevailing volumetric fluorescence microscopy.

scholarly journals Live-Cell Quantitative Imaging of Proteome Degradation by Stimulated Raman Scattering

2014 ◽  
Vol 126 (22) ◽  
pp. 5702-5705 ◽  
Author(s):  
Yihui Shen ◽  
Fang Xu ◽  
Lu Wei ◽  
Fanghao Hu ◽  
Wei Min
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Quantitative Imaging ◽  
Live Cell ◽  
Stimulated Raman
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