Development of a high throughput single-particle screening for inorganic semiconductor nanorods as neural voltage sensor

2017 ◽  
Author(s):  
Kyoungwon Park ◽  
Jack Li ◽  
Shvadchak Volodymyr ◽  
Shimon Weiss ◽  
Yung Kuo ◽  
...  
Keyword(s):  
High Throughput ◽  
Single Particle ◽  
Voltage Sensor ◽  
Inorganic Semiconductor ◽  
Particle Screening

scholarly journals Practical Considerations of Using the K3 Camera in CDS Mode for High-resolution and High-throughput Single Particle Cryo-EM

2020 ◽  
Vol 26 (S2) ◽  
pp. 1320-1320
Author(s):  
Ming Sun ◽  
Caleigh Azumaya ◽  
Eric Tse ◽  
Daniel Southworth ◽  
Kliment Verba ◽  
...  
Keyword(s):  
High Resolution ◽  
High Throughput ◽  
Single Particle

scholarly journals Nanoplastic Sizes and Numbers: Quantification by Single Particle Tracking

2020 ◽  
Author(s):  
Robert Molenaar ◽  
Swarupa Chatterjee ◽  
Mireille M. A. E Claessens ◽  
Christian Blum
Keyword(s):  
High Throughput ◽  
Particle Tracking ◽  
Single Particle ◽  
Environmental Samples ◽  
Single Particle Tracking ◽  
Water Bodies ◽  
High Throughput Analysis ◽  
Mixing Ratios ◽  
Almost Everywhere ◽  
Terrestrial Water

<p>Plastic particles have been found almost everywhere in the environment, in oceans, terrestrial water bodies, sediments and air. The extend of this unwanted contamination is difficult to fully capture. Existing quantification methods focus on the detection of millimeter to micrometer sized plastic particles, while plastic breakdown processes continue to smaller, nanometer sized, particles. For these nanoplastics methods that are inexpensive and can be (semi-) automated for high throughput analysis of dilute nanoplastic particle suspensions, are lacking. ​​​​​​​​​​​​​​Here we combine sensitive fluorescence video microsopy, NileRed staining of plastic particles, and Single Particle Tracking (SPT) to count and size nanoplastics. With this approach we show that particle diameters as low as 40 nm can be extracted, mixing ratios can be recovered, and number concentrations as low as 2·10<sup>6</sup> particles/ml can be determined. These results indicate that this approach is promising for the quantification of sizes and concentrations of nanoplastics in environmental samples.</p>

scholarly journals Emerging investigator series: automated single-nanoparticle quantification and classification: a holistic study of particles into and out of wastewater treatment plants in Switzerland

2021 ◽  
Author(s):  
Kamyar Mehrabi ◽  
Ralf Kaegi ◽  
Detlef Günther ◽  
Alexander Gundlach-Graham
Keyword(s):  
Wastewater Treatment ◽  
High Throughput ◽  
Single Particle ◽  
Wastewater Treatment Plant ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Metal Nanoparticle ◽  
Single Nanoparticle ◽  
Plant Samples

High-throughput single-particle ICP-TOFMS analyses are used to quantify and classify diverse metal nanoparticle types from wastewater treatment plant samples.

scholarly journals High-throughput single-particle tracking reveals nested membrane nanodomain organization that dictates Ras diffusion and trafficking

2019 ◽  
Author(s):  
Yerim Lee ◽  
Carey Phelps ◽  
Tao Huang ◽  
Barmak Mostofian ◽  
Lei Wu ◽  
...  
Keyword(s):  
High Throughput ◽  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Ras Signaling ◽  
Fast Diffusion ◽  
Membrane Domains ◽  
Spatial Mechanisms ◽  
Insight Into ◽  
Distinct Membrane

AbstractMembrane nanodomains have been implicated in Ras signaling, but what these domains are and how they interact with Ras remain obscure. Using high throughput single particle tracking with photoactivated localization microscopy and detailed trajectory analysis, here we show that distinct membrane domains dictate KRas diffusion and trafficking in U2OS cells. KRas exhibits an immobile state in domains ∼70 nm in size, each embedded in a larger domain (∼200 nm) that confers intermediate mobility, while the rest of the membrane supports fast diffusion. Moreover, KRas is continuously removed from the membrane via the immobile state and replenished to the fast state, likely coupled to internalization and recycling. Importantly, both the diffusion and trafficking properties of KRas remain invariant over a broad range of protein expression levels. Our results reveal how membrane organization dictates KRas diffusion and trafficking and offer insight into how Ras signaling may be regulated through spatial mechanisms.

scholarly journals Nanoplastic Sizes and Numbers: Quantification by Single Particle Tracking

2020 ◽  
Author(s):  
Robert Molenaar ◽  
Swarupa Chatterjee ◽  
Mireille M. A. E Claessens ◽  
Christian Blum
Keyword(s):  
High Throughput ◽  
Particle Tracking ◽  
Single Particle ◽  
Environmental Samples ◽  
Single Particle Tracking ◽  
Water Bodies ◽  
High Throughput Analysis ◽  
Mixing Ratios ◽  
Almost Everywhere ◽  
Terrestrial Water

<p>Plastic particles have been found almost everywhere in the environment, in oceans, terrestrial water bodies, sediments and air. The extend of this unwanted contamination is difficult to fully capture. Existing quantification methods focus on the detection of millimeter to micrometer sized plastic particles, while plastic breakdown processes continue to smaller, nanometer sized, particles. For these nanoplastics methods that are inexpensive and can be (semi-) automated for high throughput analysis of dilute nanoplastic particle suspensions, are lacking. ​​​​​​​​​​​​​​Here we combine sensitive fluorescence video microsopy, NileRed staining of plastic particles, and Single Particle Tracking (SPT) to count and size nanoplastics. With this approach we show that particle diameters as low as 40 nm can be extracted, mixing ratios can be recovered, and number concentrations as low as 2·10<sup>6</sup> particles/ml can be determined. These results indicate that this approach is promising for the quantification of sizes and concentrations of nanoplastics in environmental samples.</p>

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