scholarly journals High-Throughput Super-Resolution Single Particle Trajectory Analysis Reconstructs Organelle Dynamics and Membrane Re-Organization

2021 ◽  
Author(s):  
Pierre Parutto ◽  
Jennifer Heck ◽  
Meng Lu ◽  
Clemens Kaminski ◽  
Edward Avezov ◽  
...  
Keyword(s):  
High Throughput ◽  
Single Particle ◽  
Trajectory Analysis ◽  
Particle Trajectory ◽  
Super Resolution ◽  
Resolution Single

Anomalous Diffusion: Single Particle Trajectory Analysis

2014 ◽  
Vol 59 (8) ◽  
pp. 775-780 ◽  
Author(s):  
A. Brodin ◽  
◽  
T. Turiv ◽  
V. Nazarenko ◽  
◽  
...  
Keyword(s):  
Anomalous Diffusion ◽  
Single Particle ◽  
Trajectory Analysis ◽  
Particle Trajectory

scholarly journals Reconstructing wells from high density regions extracted from super-resolution single particle trajectories

2019 ◽  
Author(s):  
P. Parutto ◽  
J. Heck ◽  
M. Heine ◽  
D. Holcman
Keyword(s):  
Single Particle ◽  
Empirical Distribution ◽  
Basin Of Attraction ◽  
Super Resolution ◽  
High Density ◽  
Particle Trajectories ◽  
Potential Wells ◽  
Drift Field ◽  
Cellular Environment ◽  
Resolution Single

AbstractLarge amount of super-resolution single particle trajectories has revealed that the cellular environment is enriched in heterogenous regions of high density, which remain unexplained. The biophysical properties of these regions are characterized by a drift and their extension (a basin of attraction) that can be estimated from an ensemble of trajectories. We develop here two statistical methods to recover the dynamics and local potential wells (field of force and boundary) using as a model a truncated Ornstein-Ulhenbeck process. The first method uses the empirical distribution of points, which differs inside and outside the potential well, while the second focuses on recovering the drift field. Finally, we apply these two methods to voltage-gated calcium channels and phospholipids moving on the surface of neuronal cells and recover the energy and size of these high-density regions with nanometer precision.

A Fused Thiophene-S,S-Dioxide-Based Super-Photostable Fluorescent Marker for Lipid Droplets

2020 ◽  
Author(s):  
Masayasu Taki ◽  
Keiji Kajiwara ◽  
Eriko Yamaguchi ◽  
Yoshikatsu Sato ◽  
Shigehiro Yamaguchi
Keyword(s):  
Small Molecules ◽  
Lipid Droplets ◽  
Trajectory Analysis ◽  
Super Resolution ◽  
Fluorescent Marker ◽  
Selective Staining ◽  
Fluorescent Markers ◽  
Biological Studies ◽  
Particle Level

Lipid droplets (LDs) are essential organelle in most eukaryotes, and tracking intracellular LDs dynamics using synthetic small molecules is crucial for biological studies. However, only a limited number of fluorescent markers that satisfy all requirements, such as the selective staining of LDs, high photostability, and sufficient biocompatibility, have been developed. Herein, we report a series of donor-p-acceptor dyes based on the thiophene-containing fused polycyclic scaffold [1]benzothieno[3,2-<i>b</i>][1]benzothiophene (BTBT), in which either or both thiophene rings are oxidized into thiophene-<i>S</i>,<i>S</i>-dioxide to form an electron-accepting building block. Among these dyes, LAQ1 satisfied all the aforementioned requirements, and allowed us capturing ultra-small LDs on the endoplasmic reticulum (ER) membrane by stimulation emission depletion (STED) microscopy with a super-resolution below the diffraction limit of light. Moreover, the extremely high photostability of LAQ1 enabled recording the lipolysis of LDs and the concomitant lipogenesis as well as long-term trajectory analysis of micro LDs at the single particle level in living cells.

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