Multimodal quantitative phase and fluorescence imaging of cell apoptosis

2017 ◽  
Author(s):  
Chao Zuo ◽  
Hao Yan ◽  
Xinye Fu
Keyword(s):  
Fluorescence Imaging ◽  
Cell Apoptosis ◽  
Quantitative Phase

Development of multi-modal system for quantitative phase and fluorescence imaging of osteo cancer cells

2021 ◽  
Author(s):  
Shilpa Tayal ◽  
Veena Singh ◽  
Tejinder Kaur ◽  
Neetu Singh ◽  
Dalip Singh Mehta
Keyword(s):  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Modal System ◽  
Quantitative Phase

scholarly journals Isoflurane impairs oogenesis through germ cell apoptosis in C. elegans

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tao Zhang ◽  
Cheng Ni ◽  
Cheng Li ◽  
Pan Lu ◽  
Dan Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Germ Cell ◽  
Fluorescence Imaging ◽  
Cell Apoptosis ◽  
Germ Cell Apoptosis ◽  
C Elegans ◽  
Interaction Studies ◽  
Acetyl Cysteine ◽  
Induced Changes ◽  
And Oxidative Stress

AbstractAnesthetic isoflurane has been reported to induce toxicity. However, the effects of isoflurane on fecundity remain largely unknown. We established a system in C. elegans to investigate the effects of isoflurane on oogenesis. Synchronized L4 stage C. elegans were treated with 7% isoflurane for 4 h. Dead cells, ROS, embryos, and unfertilized eggs laid by hermaphrodites were measured by fluorescence imaging and counting. The C. elegans with losses of ced-3, cep-1, abl-1, male C. elegans, and oxidative stress inhibitor N-acetyl-cysteine were used in the interaction studies. We found that isoflurane decreased the numbers of embryos and unfertilized eggs and increased the levels of dead cells and ROS in C. elegans. The isoflurane-induced impairment of oogenesis was associated with abl-1, ced-3, but not cep-1. N-acetyl-cysteine attenuated the isoflurane-induced impairment of oogenesis in C. elegans. Mating with male C. elegans did not attenuate the isoflurane-induced changes in oogenesis. These findings suggest that isoflurane may impair oogenesis through abl-1- and ced-3-associated, but not cep-1-associated, germ cell apoptosis and oxidative stress, pending further investigation. These studies will promote more research to determine the potential effects of anesthesia on fecundity.

Simultaneous fluorescence imaging of selenol and hydrogen peroxide under normoxia and hypoxia in HepG2 cells and in vivo

2016 ◽  
Vol 52 (40) ◽  
pp. 6693-6696 ◽  
Author(s):  
Xiaojun Liu ◽  
Bo Hu ◽  
Ranran Cheng ◽  
Fanpeng Kong ◽  
Xiaohong Pan ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Tumor Cell ◽  
Fluorescence Imaging ◽  
Cell Apoptosis ◽  
Hepg2 Cells ◽  
Tumor Cell Apoptosis

Simultaneous fluorescence imaging of selenol and hydrogen peroxide with 5-FAM-peptide-AuNPs and QCy7-H2O2 was realized during tumor cell apoptosis induced by selenite.

Fluorescence imaging of selenol in HepG2 cell apoptosis induced by Na2SeO3

2015 ◽  
Vol 51 (15) ◽  
pp. 3102-3105 ◽  
Author(s):  
Fanpeng Kong ◽  
Bo Hu ◽  
Yan Gao ◽  
Kehua Xu ◽  
Xiaohong Pan ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Hepg2 Cell ◽  
Cell Apoptosis ◽  
Hepg2 Cells ◽  
Fluorescence Probe ◽  
Living Cells ◽  
Anticancer Mechanism

In order to investigate the Na2SeO3anticancer mechanism in HepG2 cells, a novel selenol fluorescence probe has been designed and used to image selenocysteine (Sec) in living cells andin vivo.

scholarly journals Dual-colour (near-infrared/visible) emitting annexin V for fluorescence imaging of tumour cell apoptosis in vitro and in vivo

RSC Advances ◽  
2020 ◽  
Vol 10 (63) ◽  
pp. 38244-38250
Author(s):  
Setsuko Tsuboi ◽  
Takashi Jin
Keyword(s):  
Tumor Cell ◽  
Indocyanine Green ◽  
Fluorescence Imaging ◽  
Cell Apoptosis ◽  
Near Infrared ◽  
Annexin V ◽  
Tumor Cell Apoptosis ◽  
Dual Colour

Indocyanine green labeled recombinant annexin V probes (ICG–EGFP–Annexin V and ICG–mPlum–Annexin V) were synthesized for near-infrared and visible fluorescence imaging of tumor cell apoptosis both in vitro and in vivo.

Multimodal Microscopy: Fast Acquisition of Quantitative Phase and Fluorescence Imaging in 3D Space

2021 ◽  
Vol 27 (4) ◽  
pp. 1-11
Author(s):  
Xiangyu Quan ◽  
Manoj Kumar ◽  
Sudheesh K. Rajput ◽  
Yosuke Tamada ◽  
Yasuhiro Awatsuji ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Quantitative Phase ◽  
3D Space ◽  
Fast Acquisition

The influence of confocal microscope design on imaging performance

1993 ◽  
Vol 51 ◽  
pp. 144-145
Author(s):  
C J R Sheppard
Keyword(s):  
Image Quality ◽  
Fluorescence Imaging ◽  
Confocal Microscope ◽  
Industrial Applications ◽  
Three Dimensions ◽  
Design Parameters ◽  
Weak Signals ◽  
Size And Shape ◽  
Imaging Performance ◽  
Fundamental Limitation

The confocal microscope is now widely used in both biomedical and industrial applications for imaging, in three dimensions, objects with appreciable depth. There are now a range of different microscopes on the market, which have adopted a variety of different designs. The aim of this paper is to explore the effects on imaging performance of design parameters including the method of scanning, the type of detector, and the size and shape of the confocal aperture.It is becoming apparent that there is no such thing as an ideal confocal microscope: all systems have limitations and the best compromise depends on what the microscope is used for and how it is used. The most important compromise at present is between image quality and speed of scanning, which is particularly apparent when imaging with very weak signals. If great speed is not of importance, then the fundamental limitation for fluorescence imaging is the detection of sufficient numbers of photons before the fluorochrome bleaches.

Dinuclear phosphorescent rhenium(i) complexes as potential anticancer and photodynamic therapy agents

2020 ◽  
Vol 49 (33) ◽  
pp. 11583-11590 ◽  
Author(s):  
Zheng-Yin Pan ◽  
Dai-Hong Cai ◽  
Liang He
Keyword(s):  
Photodynamic Therapy ◽  
Cell Apoptosis ◽  
Lysosomal Dysfunction ◽  
Intracellular Ros

Two dinuclear organometallic Re(i) complexes increase intracellular ROS levels, causing lysosomal dysfunction and cell apoptosis.

Ubiquinone-BODIPY nanoparticles for tumor redox-responsive fluorescence imaging and photodynamic activity

2021 ◽  
Author(s):  
Byunghee Hwang ◽  
Tae-Il Kim ◽  
Hyunjin Kim ◽  
Sungjin Jeon ◽  
Yongdoo Choi ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Selective Activation ◽  
Turn On ◽  
Intracellular Environment ◽  
Redox Responsive ◽  
Photodynamic Activity

A ubiquinone-BODIPY photosensitizer self-assembles into nanoparticles (PS-Q-NPs) and undergoes selective activation within the highly reductive intracellular environment of tumors, resulting in “turn-on” fluorescence and photosensitizing activities.

Sign in / Sign up

Export Citation Format

Share Document