Fluorescence imaging of selenol in HepG2 cell apoptosis induced by Na2SeO3

2015 ◽  
Vol 51 (15) ◽  
pp. 3102-3105 ◽  
Author(s):  
Fanpeng Kong ◽  
Bo Hu ◽  
Yan Gao ◽  
Kehua Xu ◽  
Xiaohong Pan ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Hepg2 Cell ◽  
Cell Apoptosis ◽  
Hepg2 Cells ◽  
Fluorescence Probe ◽  
Living Cells ◽  
Anticancer Mechanism

In order to investigate the Na2SeO3anticancer mechanism in HepG2 cells, a novel selenol fluorescence probe has been designed and used to image selenocysteine (Sec) in living cells andin vivo.

Simultaneous fluorescence imaging of selenol and hydrogen peroxide under normoxia and hypoxia in HepG2 cells and in vivo

2016 ◽  
Vol 52 (40) ◽  
pp. 6693-6696 ◽  
Author(s):  
Xiaojun Liu ◽  
Bo Hu ◽  
Ranran Cheng ◽  
Fanpeng Kong ◽  
Xiaohong Pan ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Tumor Cell ◽  
Fluorescence Imaging ◽  
Cell Apoptosis ◽  
Hepg2 Cells ◽  
Tumor Cell Apoptosis

Simultaneous fluorescence imaging of selenol and hydrogen peroxide with 5-FAM-peptide-AuNPs and QCy7-H2O2 was realized during tumor cell apoptosis induced by selenite.

scholarly journals Kaempferol inhibits proliferation, migration, and invasion of liver cancer HepG2 cells by down-regulation of microRNA-21

2018 ◽  
Vol 32 ◽  
pp. 205873841881434 ◽  
Author(s):  
Genglong Zhu ◽  
Xialei Liu ◽  
Haijing Li ◽  
Yang Yan ◽  
Xiaopeng Hong ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Liver Cancer ◽  
Signaling Pathway ◽  
Hepg2 Cell ◽  
Cell Apoptosis ◽  
Hepg2 Cells ◽  
Mtor Signaling ◽  
Brdu Incorporation ◽  
Migration And Invasion ◽  
Mtor Signaling Pathway

Liver cancer is one of the most common and lethal cancers in human digestive system, which kills more than half a million people every year worldwide. This study aimed to investigate the effects of kaempferol, a flavonoid compound isolated from vegetables and fruits, on hepatic cancer HepG2 cell proliferation, migration, invasion, and apoptosis, as well as microRNA-21 (miR-21) expression. Cell viability was detected using cell counting kit-8 (CCK-8) assay. Cell proliferation was measured using 5-bromo-2′-deoxyuridine (BrdU) incorporation assay. Cell apoptosis was assessed using Guava Nexin assay. Cell migration and invasion were determined using two-chamber migration (invasion) assay. Cell transfection was used to change the expression of miR-21. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to analyze the expressions of miR-21 and phosphatase and tensin homologue (PTEN). Expression of key proteins involved in proliferation, apoptosis, migration, invasion, and phosphatidylinositol 3-kinase/protein kinase 3/mammalian target of rapamycin (PI3K/AKT/mTOR) pathway were evaluated using western blotting. Results showed that kaempferol significantly inhibited HepG2 cell proliferation, migration, and invasion, and induced cell apoptosis. Kaempferol remarkably reduce the expression of miR-21 in HepG2 cells. Overexpression of miR-21 obviously reversed the effects of kaempferol on HepG2 cell proliferation, migration, invasion, and apoptosis. Moreover, miR-21 negatively regulated the expression of PTEN in HepG2 cells. Kaempferol enhanced the expression of PTEN and inactivated PI3K/AKT/mTOR signaling pathway in HepG2 cells. In conclusion, kaempferol inhibited proliferation, migration, and invasion of HepG2 cells by down-regulating miR-21 and up-regulating PTEN, as well as inactivating PI3K/AKT/mTOR signaling pathway.

scholarly journals Multifunctional selenium nanoparticles with Galangin-induced HepG2 cell apoptosis through p38 and AKT signalling pathway

2018 ◽  
Vol 5 (11) ◽  
pp. 180509 ◽  
Author(s):  
Yinghua Li ◽  
Min Guo ◽  
Zhengfang Lin ◽  
Mingqi Zhao ◽  
Yu Xia ◽  
...  
Keyword(s):  
Hepg2 Cell ◽  
Cell Apoptosis ◽  
Hepg2 Cells ◽  
Caspase 3 ◽  
Selenium Nanoparticles ◽  
Potential Candidate ◽  
Common Cancer ◽  
Anti Cancer ◽  
Surface Decoration ◽  
Cancer Activity

The morbidity and mortality of hepatocellular carcinoma, the most common cancer, are increasing continuously worldwide. Galangin (Ga) has been demonstrated to possess anti-cancer effect, but the efficacy of Ga was limited by its low permeability and poor solubility. To develop aqueous formulation and improve the anti-cancer activity of Ga, surface decoration of functionalized selenium nanoparticles with Ga (Se@Ga) was synthesized in the present study. The aim of this study was to evaluate the anti-cancer effect of Se@Ga and the mechanism on HepG2 cells. Se@Ga-induced HepG2 cell apoptosis was confirmed by depletion of mitochondrial membrane potential, translocation of phosphatidylserine and caspase-3 activation. Furthermore, Se@Ga enhanced the anti-cancer activity of HepG2 cells through ROS-mediated AKT and p38 signalling pathways. In summary, these results suggest that Se@Ga might be potential candidate chemotherapy for cancer.

Multifunctional gold nanoparticles as smart nanovehicles with enhanced tumour-targeting abilities for intracellular pH mapping and in vivo MR/fluorescence imaging

Nanoscale ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 2002-2010 ◽  
Author(s):  
Kang-Kang Yu ◽  
Kun Li ◽  
Chun-Yan Lu ◽  
Yong-Mei Xie ◽  
Yan-Hong Liu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Fluorescence Imaging ◽  
Intracellular Ph ◽  
Living Cells ◽  
Tumour Targeting ◽  
Tumour Imaging ◽  
Pathological Changes

A number of multimodal agents have been developed for tumour imaging and diagnosis, but most of them cannot be used to study the detailed physiological or pathological changes in living cells at the same time.

A cytotoxic nitrido-osmium(vi) complex induces caspase-mediated apoptosis in HepG2 cancer cells

2020 ◽  
Vol 49 (47) ◽  
pp. 17173-17182
Author(s):  
Wan-Qiong Huang ◽  
Chuan-Xian Wang ◽  
Tao Liu ◽  
Zi-Xin Li ◽  
Chen Pan ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Hepg2 Cell ◽  
Cell Apoptosis ◽  
Death Receptor ◽  
Mitochondrial Pathway ◽  
Anticancer Activities ◽  
Death Receptor Pathway

A structurally fine-tuned nitridoosmium(vi) complex induces HepG2 cell apoptosis through activation of the mitochondrial pathway and death receptor pathway, showing promising in vitro and in vivo anticancer activities.

scholarly journals A naphthalimide-based turn-on fluorescence probe for peroxynitrite detection and imaging in living cells

RSC Advances ◽  
2020 ◽  
Vol 10 (63) ◽  
pp. 38281-38286
Author(s):  
Xiling Liu ◽  
Fangyuan Gu ◽  
Xinyi Zhou ◽  
Wei Zhou ◽  
Shuping Zhang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Hepg2 Cells ◽  
Fluorescence Probe ◽  
Living Cells ◽  
Selective Detection ◽  
C Elegans ◽  
Turn On

A novel fluorescent probe HCA-OH was designed for selective detection of peroxynitrite and imaging in HepG2 cells and C. elegans.

Tracking mitochondrial viscosity in living systems based on a two-photon and near red probe

2019 ◽  
Vol 43 (43) ◽  
pp. 16945-16949 ◽  
Author(s):  
Min Peng ◽  
Junling Yin ◽  
Weiying Lin
Keyword(s):  
Fluorescence Probe ◽  
Living Cells ◽  
Living Systems ◽  
Red Emission ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence ◽  
Viscosity Changes

A two-photon fluorescence probe, Mito-V, with near-red emission to measure the viscosity changes of mitochondria in living cells and in vivo.

Imaging of living cells and zebrafish in vivo using a ratiometric fluorescent probe for hydrogen sulfide

The Analyst ◽  
2015 ◽  
Vol 140 (21) ◽  
pp. 7165-7169 ◽  
Author(s):  
Tianbao Liu ◽  
Jie Lin ◽  
Zhen Li ◽  
Lin Lin ◽  
Yuning Shen ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescent Probe ◽  
Fluorescence Probe ◽  
Living Cells ◽  
Ratiometric Fluorescence ◽  
Ratiometric Fluorescent Probe

We have developed a novel colorimetric and ratiometric fluorescence probe for the selective and sensitive imaging of H2S in living cells and living zebrafish in vivo.

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