Determination of three-dimensional molecular orientation of type-I collagen by circularly-polarized second harmonic generation imaging

2017 ◽  
Author(s):  
Guan-Yu Zhuo ◽  
Wei-Han Hung ◽  
Fu-Jen Kao
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Molecular Orientation ◽  
Type I Collagen ◽  
Second Harmonic ◽  
Three Dimensional ◽  
Type I ◽  
Circularly Polarized ◽  
Second Harmonic Generation Imaging

THE INFLUENCE OF THE ORIENTATION OF THE COLLAGEN DIPOLES ON SECOND HARMONIC GENERATION (SHG) WITH HIGH NA UNDER CRYSTALLIZED TYPE I COLLAGEN FIBER MODEL

2012 ◽  
Vol 26 (29) ◽  
pp. 1250148
Author(s):  
FEI FEI WANG ◽  
JIAN MEI LIU ◽  
XIAO YUAN DENG
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Collagen Fiber ◽  
Type I Collagen ◽  
Second Harmonic ◽  
Numerical Aperture ◽  
Orientation Angle ◽  
Type I ◽  
Dipole Orientation ◽  
First Time

In this paper, for the first time, we consider theoretically all the potential orientations of dipoles for second harmonic generation (SHG) under crystallized type I collagen fiber. With high numerical aperture ( NA = 14), the effect of dipole orientation angle Φ on the SHG intensity, on the maximum SHG emergence angles (θ max , φ max ) and the action of these dipole sources on the ratio of forward to backward (F/B) SHG have been studied under different cases of fibrils diameter d1 and the QPM order (m, l) in the crystallized collagen fiber, and it is found that the influential patterns of the same Φ on SHG at different fibrils diameter d1 and the QPM order (m, l) are different. The dipoles arrangement is related to the characteristic changes in biological tissue so that our work may provide a helpful theoretical tool for pathological status detecting.

Second-harmonic-generation microscope using eight-segment polarization-mode converter to observe three-dimensional molecular orientation

2007 ◽  
Vol 32 (12) ◽  
pp. 1680 ◽  
Author(s):  
Keisuke Yoshiki ◽  
Kanamaru Ryosuke ◽  
Mamoru Hashimoto ◽  
Nobuyuki Hashimoto ◽  
Tsutomu Araki
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Molecular Orientation ◽  
Second Harmonic ◽  
Three Dimensional ◽  
Mode Converter ◽  
Polarization Mode

scholarly journals Second Harmonic Generation in 3-D Uniform Arrangement of Type I Collagen on Nonlinear Optics Microscopy

Scanning ◽  
2013 ◽  
Vol 35 (1) ◽  
pp. 12-16 ◽  
Author(s):  
Z. F. Zhuang ◽  
M. F. Zhu ◽  
Z.Y. Guo ◽  
S. H. Liu
Keyword(s):  
Nonlinear Optics ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Type I Collagen ◽  
Second Harmonic ◽  
Type I
Sign in / Sign up

Export Citation Format

Share Document