Fluorescence lifetime imaging using a single photon avalanche diode array sensor (Conference Presentation)

2017 ◽  
Author(s):  
Piotr M. Wargocki ◽  
David J. Spence ◽  
Ewa M. Goldys ◽  
Edoardo Charbon ◽  
Claudio E. Bruschini ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Single Photon ◽  
Diode Array ◽  
Fluorescence Lifetime Imaging ◽  
Avalanche Diode ◽  
Lifetime Imaging ◽  
Array Sensor

scholarly journals Real-time fluorescence lifetime imaging system with a 32 × 32 013μm CMOS low dark-count single-photon avalanche diode array

2010 ◽  
Vol 18 (10) ◽  
pp. 10257 ◽  
Author(s):  
Day-Uei Li ◽  
Jochen Arlt ◽  
Justin Richardson ◽  
Richard Walker ◽  
Alex Buts ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescence Lifetime ◽  
Imaging System ◽  
Single Photon ◽  
Diode Array ◽  
Fluorescence Lifetime Imaging ◽  
Dark Count ◽  
Avalanche Diode ◽  
Lifetime Imaging

scholarly journals Real-time multispectral fluorescence lifetime imaging using Single Photon Avalanche Diode arrays

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
João L. Lagarto ◽  
Federica Villa ◽  
Simone Tisa ◽  
Franco Zappa ◽  
Vladislav Shcheslavskiy ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescence Lifetime ◽  
Single Photon ◽  
Fluorescence Lifetime Imaging ◽  
Avalanche Diode ◽  
Lifetime Imaging

scholarly journals Video-rate fluorescence lifetime imaging camera with CMOS single-photon avalanche diode arrays and high-speed imaging algorithm

2011 ◽  
Vol 16 (9) ◽  
pp. 096012 ◽  
Author(s):  
David D.-U. Li ◽  
Jochen Arlt ◽  
David Tyndall ◽  
Richard Walker ◽  
Justin Richardson ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
High Speed ◽  
Single Photon ◽  
Fluorescence Lifetime Imaging ◽  
High Speed Imaging ◽  
Imaging Algorithm ◽  
Avalanche Diode ◽  
Lifetime Imaging ◽  
Imaging Camera

scholarly journals Optical Estimation of Absolute Membrane Potential Using One- and Two-Photon Fluorescence Lifetime Imaging Microscopy

2021 ◽  
Author(s):  
Julia R. Lazzari-Dean ◽  
Evan W. Miller
Keyword(s):  
Membrane Potential ◽  
Fluorescence Lifetime ◽  
Single Photon ◽  
Photon Counting ◽  
Fluorescence Lifetime Imaging ◽  
Single Photon Counting ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Wide Range ◽  
Relative Measurements

AbstractBackgroundMembrane potential (Vmem) exerts physiological influence across a wide range of time and space scales. To study Vmem in these diverse contexts, it is essential to accurately record absolute values of Vmem, rather than solely relative measurements.Materials & MethodsWe use fluorescence lifetime imaging of a small molecule voltage sensitive dye (VF2.1.Cl) to estimate mV values of absolute membrane potential.ResultsWe test the consistency of VF2.1.Cl lifetime measurements performed on different single photon counting instruments and find that they are in striking agreement (differences of <0.5 ps/mV in the slope and <50 ps in the y-intercept). We also demonstrate that VF2.1.Cl lifetime reports absolute Vmem under two-photon (2P) illumination with better than 20 mV of Vmem resolution, a nearly 10-fold improvement over other lifetime-based methods.ConclusionsWe demonstrate that VF-FLIM is a robust and portable metric for Vmem across imaging platforms and under both one-photon and two-photon illumination. This work is a critical foundation for application of VF-FLIM to record absolute membrane potential signals in thick tissue.

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