Pump-probe microscopy of respiratory chain pigments: towards non-fluorescent label-free metabolic imaging

2017 ◽  
Author(s):  
Scott R. Domingue ◽  
Adam J. Chicco ◽  
Randy A. Bartels ◽  
Jesse W. Wilson
Keyword(s):  
Respiratory Chain ◽  
Metabolic Imaging ◽  
Fluorescent Label ◽  
Label Free ◽  
Pump Probe ◽  
Probe Microscopy

scholarly journals Label-Free Imaging of Female Genital Tract Melanocytic Lesions With Pump-Probe Microscopy

2017 ◽  
Vol 21 (2) ◽  
pp. 137-144 ◽  
Author(s):  
Francisco E. Robles ◽  
Sanghamitra Deb ◽  
Martin C. Fischer ◽  
Warren S. Warren ◽  
Maria Angelica Selim
Keyword(s):  
Genital Tract ◽  
Female Genital Tract ◽  
Label Free ◽  
Pump Probe ◽  
Probe Microscopy ◽  
Melanocytic Lesions ◽  
Female Genital

scholarly journals Label-free imaging of hemoglobin degradation and hemosiderin formation in brain tissues with femtosecond pump-probe microscopy

Theranostics ◽  
2018 ◽  
Vol 8 (15) ◽  
pp. 4129-4140 ◽  
Author(s):  
Lili Zhang ◽  
Xiang Zou ◽  
Bohan Zhang ◽  
Liyuan Cui ◽  
Jiayi Zhang ◽  
...  
Keyword(s):  
Label Free ◽  
Pump Probe ◽  
Probe Microscopy ◽  
Hemoglobin Degradation ◽  
Brain Tissues

scholarly journals Label-free, High-Resolution Optical Metabolic Imaging of Human Cervical Precancers Reveals Potential for Intraepithelial Neoplasia Diagnosis

2020 ◽  
Vol 1 (2) ◽  
pp. 100017
Author(s):  
Dimitra Pouli ◽  
Hong-Thao Thieu ◽  
Elizabeth M. Genega ◽  
Laura Baecher-Lind ◽  
Michael House ◽  
...  
Keyword(s):  
High Resolution ◽  
Intraepithelial Neoplasia ◽  
Metabolic Imaging ◽  
Label Free

scholarly journals Imaging theory of structured pump-probe microscopy

2016 ◽  
Vol 24 (18) ◽  
pp. 20868 ◽  
Author(s):  
Eric S. Massaro ◽  
Andrew H. Hill ◽  
Casey L. Kennedy ◽  
Erik M. Grumstrup
Keyword(s):  
Pump Probe ◽  
Probe Microscopy ◽  
Imaging Theory

scholarly journals Label-free and live cell imaging by interferometric scattering microscopy

2018 ◽  
Vol 9 (10) ◽  
pp. 2690-2697 ◽  
Author(s):  
Jin-Sung Park ◽  
Il-Buem Lee ◽  
Hyeon-Min Moon ◽  
Jong-Hyeon Joo ◽  
Kyoung-Hoon Kim ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Complex Structure ◽  
Live Cell ◽  
Fluorescent Label ◽  
Live Cells ◽  
Label Free ◽  
Cytoplasmic Organelles ◽  
Microscopic Techniques

Despite recent remarkable advances in microscopic techniques, it still remains very challenging to directly observe the complex structure of cytoplasmic organelles in live cells without a fluorescent label.

Fluorescent Label-Free Aptasensor Integrated in a Lab-on-Chip System for the Detection of Ochratoxin A in Beer and Wheat

2019 ◽  
Vol 2 (12) ◽  
pp. 5880-5887 ◽  
Author(s):  
Francesca Costantini ◽  
Nicola Lovecchio ◽  
Albert Ruggi ◽  
Cesare Manetti ◽  
Augusto Nascetti ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Fluorescent Label ◽  
Label Free ◽  
Lab On Chip ◽  
On Chip

scholarly journals 3D extracellular matrix microenvironment in bioengineered tissue models of primary pediatric and adult brain tumors

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Disha Sood ◽  
Min Tang-Schomer ◽  
Dimitra Pouli ◽  
Craig Mizzoni ◽  
Nicole Raia ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Brain Tumor ◽  
Brain Tumors ◽  
Adult Brain ◽  
Metabolic Imaging ◽  
Tumor Type ◽  
Label Free ◽  
Pediatric Ependymoma ◽  
Extracellular Lipid ◽  
Tumor Cell Behavior

Abstract Dynamic alterations in the unique brain extracellular matrix (ECM) are involved in malignant brain tumors. Yet studies of brain ECM roles in tumor cell behavior have been difficult due to lack of access to the human brain. We present a tunable 3D bioengineered brain tissue platform by integrating microenvironmental cues of native brain-derived ECMs and live imaging to systematically evaluate patient-derived brain tumor responses. Using pediatric ependymoma and adult glioblastoma as examples, the 3D brain ECM-containing microenvironment with a balance of cell-cell and cell-matrix interactions supports distinctive phenotypes associated with tumor type-specific and ECM-dependent patterns in the tumor cells’ transcriptomic and release profiles. Label-free metabolic imaging of the composite model structure identifies metabolically distinct sub-populations within a tumor type and captures extracellular lipid-containing droplets with potential implications in drug response. The versatile bioengineered 3D tumor tissue system sets the stage for mechanistic studies deciphering microenvironmental role in brain tumor progression.

scholarly journals Femtosecond pump-probe microscopy generates virtual cross-sections in historic artwork

2014 ◽  
Vol 111 (5) ◽  
pp. 1708-1713 ◽  
Author(s):  
Tana Elizabeth Villafana ◽  
William P. Brown ◽  
John K. Delaney ◽  
Michael Palmer ◽  
Warren S. Warren ◽  
...  
Keyword(s):  
Cross Sections ◽  
Pump Probe ◽  
Probe Microscopy
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