Selective two-photon collagen crosslinking in situ measured by Brillouin microscopy (Conference Presentation)

2017 ◽  
Author(s):  
Sheldon J. J. Kwok ◽  
Ivan A. Kuznetsov ◽  
Moonseok Kim ◽  
Myunghwan Choi ◽  
Giuliano Scarcelli ◽  
...  
Keyword(s):  
Collagen Crosslinking ◽  
Two Photon

scholarly journals Selective two-photon collagen crosslinking in situ measured by Brillouin microscopy

Optica ◽  
2016 ◽  
Vol 3 (5) ◽  
pp. 469 ◽  
Author(s):  
Sheldon J. J. Kwok ◽  
Ivan A. Kuznetsov ◽  
Moonseok Kim ◽  
Myunghwan Choi ◽  
Giuliano Scarcelli ◽  
...  
Keyword(s):  
Collagen Crosslinking ◽  
Two Photon

scholarly journals Polarity-active NIR probes with strong two-photon absorption and ultrahigh binding affinity of insulin amyloid fibrils

2021 ◽  
Author(s):  
Li Li ◽  
Zheng Lv ◽  
Zhongwei Man ◽  
Zhenzhen Xu ◽  
YuLing Wei ◽  
...  
Keyword(s):  
Neurodegenerative Diseases ◽  
Binding Affinity ◽  
Fluorescent Probes ◽  
Amyloid Fibrils ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Medical Diagnostic

Amyloid fibrils are associated with many neurodegenerative diseases. In-situ and in-vivo visualization of amyloid fibrils is important for medical diagnostic and requires fluorescent probes with both excitation and emission wavelengths in...

Determination of absolute two-photon excitation cross sections by in situ second-order autocorrelation

1995 ◽  
Vol 20 (23) ◽  
pp. 2372 ◽  
Author(s):  
Chris Xu ◽  
Winfried Denk ◽  
Jeffrey Guild ◽  
Watt W. Webb
Keyword(s):  
Cross Sections ◽  
Second Order ◽  
Two Photon ◽  
Photon Excitation ◽  
Excitation Cross Sections ◽  
Two Photon Excitation

A New Pre-Embedding Immunogold Method That Permits to Obtain a Very High Signal with a Very Good Ultrastucture

2001 ◽  
Vol 7 (S2) ◽  
pp. 1044-1045
Author(s):  
G. Grondin ◽  
A.R. Bcaudoin
Keyword(s):  
New Technologies ◽  
Immunofluorescence Microscopy ◽  
High Signal ◽  
Sodium Metaperiodate ◽  
Two Photon ◽  
Cacodylate Buffer ◽  
Ultrastructure Preservation ◽  
High Level ◽  
Very High

The ultimate goal of the immunocytochemistry is to get the best signal with the lowest background while preserving ultrastructure. Unfortunaly too often the experimentator faces a compromise between density of the signal and ultrastructure preservation (1). with the advent of hight resolution confocal light microscopy, two photon imaging and other new technologies there is a need to provide the best immunocytochemical complementary results with the high resolution of the electron microscope. But often for exemple the high level of labelling observed by immunofluorescence microscopy is not matched by immunocytochemistry (2). One approach to obtain comparable levels of labelling is to use comparable protocols. We propose here such a new immunogold method. to illustrate our point we used a very convenient biological material, the adherent cells grown on 35 mm plastic culture dishes. The following procedure was applied : Confluent endothelial cells were fixed in situ for 3 hours with the following freshly prepared and filtered solution : 1 % 1-lysine, 4% paraformaldehyde, 0.04% glutaraldehyde, 0.25% sodium metaperiodate in 0.04M sodium cacodylate buffer, pH 7.4.

Two-Photon Induced Uncaging of a Reactive Intermediate. Multiphoton In Situ Detection of a Potentially Valuable Label for Biological Applications

2005 ◽  
Vol 70 (6) ◽  
pp. 2143-2147 ◽  
Author(s):  
Joanne Dyer ◽  
Steffen Jockusch ◽  
Vojtech Balsanek ◽  
Dalibor Sames ◽  
Nicholas J. Turro
Keyword(s):  
Biological Applications ◽  
Reactive Intermediate ◽  
Two Photon ◽  
In Situ Detection

scholarly journals Measurement and analysis of sarcomere length in rat cardiomyocytes in situ and in vitro

2010 ◽  
Vol 298 (5) ◽  
pp. H1616-H1625 ◽  
Author(s):  
G. Bub ◽  
P. Camelliti ◽  
C. Bollensdorff ◽  
D. J. Stuckey ◽  
G. Picton ◽  
...  
Keyword(s):  
Measurement Errors ◽  
Sarcomere Length ◽  
Ex Vivo ◽  
Living Cells ◽  
Perfused Heart ◽  
Experimental Conditions ◽  
Rat Cardiomyocytes ◽  
Two Photon

Sarcomere length (SL) is an important determinant and indicator of cardiac mechanical function; however, techniques for measuring SL in living, intact tissue are limited. Here, we present a technique that uses two-photon microscopy to directly image striations of living cells in cardioplegic conditions, both in situ (Langendorff-perfused rat hearts and ventricular tissue slices, stained with the fluorescent marker di-4-ANEPPS) and in vitro (acutely isolated rat ventricular myocytes). Software was developed to extract SL from two-photon fluorescence image sets while accounting for measurement errors associated with motion artifact in raster-scanned images and uncertainty of the cell angle relative to the imaging plane. Monte-Carlo simulations were used to guide analysis of SL measurements by determining error bounds as a function of measurement path length. The mode of the distribution of SL measurements in resting Langendorff-perfused heart is 1.95 μm ( n = 167 measurements from N = 11 hearts) after correction for tissue orientation, which was significantly greater than that in isolated cells (1.71 μm, n = 346, N = 9 isolations) or ventricular slice preparations (1.79 μm, n = 79, N = 3 hearts) under our experimental conditions. Furthermore, we find that edema in arrested Langendorff-perfused heart is associated with a mean SL increase; this occurs as a function of time ex vivo and correlates with tissue volume changes determined by magnetic resonance imaging. Our results highlight that the proposed method can be used to monitor SL in living cells and that different experimental models from the same species may display significantly different SL values under otherwise comparable conditions, which has implications for experiment design, as well as comparison and interpretation of data.

scholarly journals Two-Photon Immunofluorescence Characterization of the Trabecular Meshwork In Situ

2012 ◽  
Vol 53 (7) ◽  
pp. 3395 ◽  
Author(s):  
Jose M. Gonzalez ◽  
Martin Heur ◽  
James C. H. Tan
Keyword(s):  
Trabecular Meshwork ◽  
Two Photon
Sign in / Sign up

Export Citation Format

Share Document