Accurate live and dead bacterial cell enumeration using flow cytometry (Conference Presentation)

2017 ◽  
Author(s):  
Fang Ou ◽  
Cushla McGoverin ◽  
Simon Swift ◽  
Frédérique Vanholsbeeck
Keyword(s):  
Flow Cytometry ◽  
Bacterial Cell ◽  
Cell Enumeration

scholarly journals Absolute bacterial cell enumeration using flow cytometry

2017 ◽  
Vol 123 (2) ◽  
pp. 464-477 ◽  
Author(s):  
F. Ou ◽  
C. McGoverin ◽  
S. Swift ◽  
F. Vanholsbeeck
Keyword(s):  
Flow Cytometry ◽  
Bacterial Cell ◽  
Cell Enumeration

Bacterial cell enumeration using flow cytometry

2016 ◽  
Author(s):  
Fang Ou ◽  
Cushla McGoverin ◽  
Simon Swift ◽  
Frédérique Vanholsbeeck
Keyword(s):  
Flow Cytometry ◽  
Bacterial Cell ◽  
Cell Enumeration

scholarly journals Enumeration of Bacterial Cell Numbers and Detection of Significant Bacteriuria by Use of a New Flow Cytometry-Based Device

2006 ◽  
Vol 44 (10) ◽  
pp. 3596-3599 ◽  
Author(s):  
H. Okada ◽  
T. Shirakawa ◽  
A. Gotoh ◽  
Y. Kamiyama ◽  
S. Muto ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Bacterial Cell ◽  
Cell Numbers ◽  
Significant Bacteriuria

scholarly journals Use of Formalin-Fixed, Propidium Iodide-Stained Human Leukocytes as a Standard for Enumerating CD4+ T Lymphocytes in a Single-Platform Assay

2001 ◽  
Vol 8 (2) ◽  
pp. 397-401 ◽  
Author(s):  
G. M. Harrison ◽  
A. J. Bennett ◽  
M. Moody ◽  
G. F. Read ◽  
P. E. Williams
Keyword(s):  
Flow Cytometry ◽  
Blood Cell ◽  
Propidium Iodide ◽  
Internal Standard ◽  
Inexpensive Method ◽  
Normal Cells ◽  
Blood Samples ◽  
Cell Enumeration ◽  
Whole Blood Samples ◽  
Formalin Fixed

ABSTRACT A new, inexpensive method is described that enables lymphocytes to be enumerated very precisely. Normal leukocytes were simultaneously stained and fixed with a propidium iodide-paraformaldehyde solution. The preparation obtained (CellBeads) was used as an internal standard for cell enumeration by flow cytometry and was stable at 4°C for at least 60 days. Unlike synthetic beads, the CellBeads behaved similarly to normal cells during red blood cell lysis and cell washing procedures. When known numbers of CellBeads were added to whole-blood samples and the numbers of CellBeads and lymphocytes were determined, highly reproducible and accurate enumerations were obtained—far more so than when synthetic beads were used. This inexpensive method is suitable for routine use.

Bacterial Cell Monitoring in Wastewater Treatment Plants by Flow Cytometry

2008 ◽  
Vol 80 (4) ◽  
pp. 346-354 ◽  
Author(s):  
Anita Manti ◽  
Paola Boi ◽  
Tania Falcioni ◽  
Barbara Canonico ◽  
Alessia Ventura ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Wastewater Treatment ◽  
Bacterial Cell ◽  
Wastewater Treatment Plants ◽  
Cell Monitoring

Enumeration of viable CD34+ cells by flow cytometry in blood, bone marrow and cord blood: results of a study of the novel BD™ stem cell enumeration kit

Cytotherapy ◽  
2011 ◽  
Vol 13 (4) ◽  
pp. 449-458 ◽  
Author(s):  
Katrin Dauber ◽  
Daniela Becker ◽  
Marcus Odendahl ◽  
Erhard Seifried ◽  
Halvard Bonig ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
Stem Cell ◽  
Cord Blood ◽  
The Novel ◽  
Cd34 Cells ◽  
Cell Enumeration
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