scholarly journals Opportunities for the application of real‐time bacterial cell analysis using flow cytometry for the advancement of sterilization microbiology

2020 ◽  
Author(s):  
B. McEvoy ◽  
M. Lynch ◽  
N.J. Rowan
Keyword(s):  
Flow Cytometry ◽  
Real Time ◽  
Bacterial Cell ◽  
Cell Analysis

scholarly journals T3 Up-Regulates PDZK1 to Promote the Proliferation of Papillary Thyroid Cancer Cells

2021 ◽  
Author(s):  
Yan Wang ◽  
Yanan Cheng ◽  
Rongxin Sun ◽  
Jianan Lang ◽  
Longyan Yang ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Proliferation ◽  
Thyroid Cancer ◽  
Protein Expression ◽  
Real Time ◽  
Papillary Thyroid Cancer ◽  
Immunofluorescence Staining ◽  
Cell Analysis ◽  
Papillary Thyroid ◽  
Rna Seq

Abstract Aim: Thyroid cancer (TC) is the most common malignant tumor of the endocrine system. Studies have showed that Triiodothyronine (T3) promotes the proliferation of papillary thyroid cancer (PTC) cells, but the specific mechanism remains unclear. Several studies have showed that PDZK1 played important roles in the occurrence and development of cancer. However, the biological function of PDZK1 in PTC remains unclear. Therefore, the aims of this study were to investigate the effect of PDZK1 in PTC and the underlying mechanism.Materials and Methods: The effect of T3 on proliferation of PTC cell (TPC-1) was analyzed by colony formation assay and real time cell analysis. Immunofluorescence staining aimed to analyze protein expression. RNA-seq was used to analyze the expression of PDZK1. Meanwhile, Western blot was used to verify the protein expression. The effect of PDZK1 on PTC cell proliferation was investigated by Cell Counting Kit-8(CCK8), real time cell analysis, flow cytometry and transwell assay, respectively. Results: We found that T3 increased the expression of PDZK1 in TPC-1 cells and promote the proliferation of TPC-1 cells which can be weakened after PDZK1 was knocked down. Immunofluorescence staining showed that the expression of PDZK1 was higher in PTC than paracancerous tissues. And the analysis of 6 benign thyroid nodules and 4 thyroid cancer tissues by RNA-Seq showed that the expression of PDZK1 was increased in PTC tissues, the expression of PDZK1 was also increased in PTC cells compared with the normal thyroid epithelium. In addition, PDZK1 promoted TPC-1 cell proliferation was detected by real-time cell analysis (RTCA) and CCK8. Flow cytometry analysis showed that PDZK1 increased cell cycle at S phase and decreased at G1 phase of TPC-1. PDZK1 promoted the invasion of TPC-1 cell was tested by transwell. Conclusion: These results suggest that T3 can promote the proliferation of PTC cells, which may be mediated by PDZK1. This study illustrates a possible mechanism by which T3 promotes the proliferation of PTC and provides a theoretical basis for the prevention and treatment of PTC.

Real-time monitoring of circulating tumor cell release during tumor manipulation using in vivo photoacoustic and fluorescent flow cytometry

Head & Neck ◽  
2013 ◽  
Vol 36 (8) ◽  
pp. 1207-1215 ◽  
Author(s):  
Mazen A. Juratli ◽  
Mustafa Sarimollaoglu ◽  
Eric R. Siegel ◽  
Dmitry A. Nedosekin ◽  
Ekaterina I. Galanzha ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Tumor Cell ◽  
Real Time ◽  
Circulating Tumor Cell ◽  
Real Time Monitoring ◽  
Cell Release

scholarly journals Touch Imprint Intraoperative Flow Cytometry as a Complementary Tool for Detailed Assessment of Resection Margins and Tumor Biology in Liver Surgery for Primary and Metastatic Liver Neoplasms

2021 ◽  
Vol 4 (3) ◽  
pp. 66
Author(s):  
Georgios S. Markopoulos ◽  
Georgios K. Glantzounis ◽  
Anna C. Goussia ◽  
Georgios D. Lianos ◽  
Anastasia Karampa ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Liver Tumors ◽  
Tumor Biology ◽  
Surgical Margins ◽  
Distribution Analysis ◽  
Cell Analysis ◽  
Term Survival ◽  
Detailed Assessment ◽  
Metastatic Liver

Liver resection is the main treatment for primary and metastatic liver tumors in order to achieve long-term survival with good quality of life. The ultimate goal of surgical oncology is to achieve complete tumor removal with adequate clear surgical margins. Flow cytometry is a powerful analytical technique with applications such as phenotypic analysis and quantification of DNA content. Intraoperative flow cytometry (iFC) is the application of flow cytometry for DNA content/ploidy and cell cycle distribution analysis during surgery for tumor cell analysis and margin evaluation. It has been used for cell analysis of intracranial tumors and recently of head and neck carcinomas and breast carcinomas, as well as for tumor margin evaluation. Herein, we present a novel touch imprint iFC protocol for the detailed assessment of tumor margins during excision of malignant hepatic lesions. The protocol aims to offer information on surgical margins after removal of malignant liver tumors based on DNA content of cancer cells and to corroborate the results of iFC with that of histopathological analysis. Based on the established role of iFC in other types of malignancies, our specialized protocol has the potential, through characterization of cells in liver transection surface post hepatectomy, to offer significant information on the type of resection and tumor biology. This information can be used to effectively guide intra- and postoperative patient management.

scholarly journals Necrotrophic Growth of Legionella pneumophila

2006 ◽  
Vol 72 (6) ◽  
pp. 4323-4328 ◽  
Author(s):  
R. Temmerman ◽  
H. Vervaeren ◽  
B. Noseda ◽  
N. Boon ◽  
W. Verstraete
Keyword(s):  
Flow Cytometry ◽  
Real Time ◽  
Legionella Pneumophila ◽  
Real Time Pcr ◽  
Water Systems ◽  
Saccharomyces Boulardii ◽  
Wall Structure ◽  
Lower Growth ◽  
Average Growth ◽  
Microbial Cells

ABSTRACT This study examined whether Legionella pneumophila is able to thrive on heat-killed microbial cells (necrotrophy) present in biofilms or heat-treated water systems. Quantification by means of plate counting, real-time PCR, and flow cytometry demonstrated necrotrophic growth of L. pneumophila in water after 96 h, when at least 100 dead cells are available to one L. pneumophila cell. Compared to the starting concentration of L. pneumophila, the maximum observed necrotrophic growth was 1.89 log units for real-time PCR and 1.49 log units for plate counting. The average growth was 1.57 � 0.32 log units (n = 5) for real-time PCR and 1.14 � 0.35 log units (n = 5) for plate counting. Viability staining and flow cytometry showed that the fraction of living cells in the L. pneumophila population rose from the initial 54% to 82% after 96 h. Growth was measured on heat-killed Pseudomonas putida, Escherichia coli, Acanthamoeba castellanii, Saccharomyces boulardii, and a biofilm sample. Gram-positive organisms did not result in significant growth of L. pneumophila, probably due to their robust cell wall structure. Although necrotrophy showed lower growth yields compared to replication within protozoan hosts, these findings indicate that it may be of major importance in the environmental persistence of L. pneumophila. Techniques aimed at the elimination of protozoa or biofilm from water systems will not necessarily result in a subsequent removal of L. pneumophila unless the formation of dead microbial cells is minimized.

scholarly journals Enumeration of Bacterial Cell Numbers and Detection of Significant Bacteriuria by Use of a New Flow Cytometry-Based Device

2006 ◽  
Vol 44 (10) ◽  
pp. 3596-3599 ◽  
Author(s):  
H. Okada ◽  
T. Shirakawa ◽  
A. Gotoh ◽  
Y. Kamiyama ◽  
S. Muto ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Bacterial Cell ◽  
Cell Numbers ◽  
Significant Bacteriuria

Effect of antibiotics on biofilm inhibition and induction measured by real-time cell analysis

2017 ◽  
Vol 122 (3) ◽  
pp. 640-650 ◽  
Author(s):  
M.D. Ferrer ◽  
J.C. Rodriguez ◽  
L. Álvarez ◽  
A. Artacho ◽  
G. Royo ◽  
...  
Keyword(s):  
Real Time ◽  
Cell Analysis ◽  
Biofilm Inhibition
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