High-speed stimulated Raman scattering microscopy for studying the metabolic diversity of motile Euglena gracilis

2017 ◽  
Author(s):  
Y. Suzuki ◽  
Y. Wakisaka ◽  
O. Iwata ◽  
A. Nakashima ◽  
T. Ito ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Euglena Gracilis ◽  
High Speed ◽  
Stimulated Raman Scattering ◽  
Metabolic Diversity ◽  
Stimulated Raman

scholarly journals Label-free chemical imaging flow cytometry by high-speed multicolor stimulated Raman scattering

2019 ◽  
Vol 116 (32) ◽  
pp. 15842-15848 ◽  
Author(s):  
Yuta Suzuki ◽  
Koya Kobayashi ◽  
Yoshifumi Wakisaka ◽  
Dinghuan Deng ◽  
Shunji Tanaka ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Raman Scattering ◽  
Cancer Biology ◽  
High Speed ◽  
Stimulated Raman Scattering ◽  
Chemical Imaging ◽  
Fluorescent Labeling ◽  
Label Free ◽  
Imaging Flow Cytometry ◽  
Stimulated Raman

Combining the strength of flow cytometry with fluorescence imaging and digital image analysis, imaging flow cytometry is a powerful tool in diverse fields including cancer biology, immunology, drug discovery, microbiology, and metabolic engineering. It enables measurements and statistical analyses of chemical, structural, and morphological phenotypes of numerous living cells to provide systematic insights into biological processes. However, its utility is constrained by its requirement of fluorescent labeling for phenotyping. Here we present label-free chemical imaging flow cytometry to overcome the issue. It builds on a pulse pair-resolved wavelength-switchable Stokes laser for the fastest-to-date multicolor stimulated Raman scattering (SRS) microscopy of fast-flowing cells on a 3D acoustic focusing microfluidic chip, enabling an unprecedented throughput of up to ∼140 cells/s. To show its broad utility, we use the SRS imaging flow cytometry with the aid of deep learning to study the metabolic heterogeneity of microalgal cells and perform marker-free cancer detection in blood.

Effect of Fiber-Optics Nonlinearities in Long Haul and Ultra-High Speed DWDM Optical Transmission Networks at 10, 40 and 100 Gb/s Ultra-High Speed Data Rates

2018 ◽  
Vol 0 (0) ◽  
Author(s):  
Farman Ali ◽  
Yousaf Khan ◽  
Shahryar Shafique Qureshi ◽  
Shabir Ahmad ◽  
Muhammad Waqas
Keyword(s):  
Raman Scattering ◽  
Fiber Optics ◽  
Wavelength Division Multiplexing ◽  
High Speed ◽  
Stimulated Raman Scattering ◽  
Ultra High Speed ◽  
Data Rates ◽  
High Speed Data ◽  
The Impact ◽  
Stimulated Raman

AbstractThe impact of major nonlinearities in optical fiber such as SRR (Stimulated Raman Scattering) and Four Wave Maxing (FWM) in cascaded amplifier Dense Wavelength Division Multiplexing optical long reach and high speed data rate transmission scheme have been investigated at different data rates. Pulse walk off effect is considered for the purpose of calculating Stimulated Raman Scattering (SRS) nonlinear factor. Examination has been accomplished to assess signal to noise ratio thinking about the consolidated impact of SRS and FWM within the sight of amplified spontaneous emission noise to achieve minutest noise at altered data rates.

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