scholarly journals An optical tweezers, epi-fluorescence and microfluidic-setup for synchronization studies of glycolytic oscillations in living yeast cells

2016 ◽  
Author(s):  
Martin Mojica-Benavides ◽  
Amin A. Banaeiyan ◽  
David D. van Niekerk ◽  
Jacky L. Snoep ◽  
Anna-Karin Gustavsson ◽  
...  
2018 ◽  
Vol 82 (1) ◽  
Author(s):  
Anna‐Karin Gustavsson ◽  
Amin A. Banaeiyan ◽  
David D. Niekerk ◽  
Jacky L. Snoep ◽  
Caroline B. Adiels ◽  
...  

Nanophotonics ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Liuhao Zhu ◽  
Miaomiao Tang ◽  
Hehe Li ◽  
Yuping Tai ◽  
Xinzhong Li

Abstract Generally, an optical vortex lattice (OVL) is generated via the superposition of two specific vortex beams. Thus far, OVL has been successfully employed to trap atoms via the dark cores. The topological charge (TC) on each optical vortex (OV) in the lattice is only ±1. Consequently, the orbital angular momentum (OAM) on the lattice is ignored. To expand the potential applications, it is necessary to rediscover and exploit OAM. Here we propose a novel high-order OVL (HO-OVL) that combines the phase multiplication and the arbitrary mode-controllable techniques. TC on each OV in the lattice is up to 51, which generates sufficient OAM to manipulate microparticles. Thereafter, the entire lattice can be modulated to desirable arbitrary modes. Finally, yeast cells are trapped and rotated by the proposed HO-OVL. To the best of our knowledge, this is the first realization of the complex motion of microparticles via OVL. Thus, this work successfully exploits OAM on OVL, thereby revealing potential applications in particle manipulation and optical tweezers.


2015 ◽  
Vol 25 (6) ◽  
pp. 064606 ◽  
Author(s):  
Takashi Amemiya ◽  
Kouhei Obase ◽  
Naoki Hiramatsu ◽  
Kiminori Itoh ◽  
Kenichi Shibata ◽  
...  

Author(s):  
Peter Richard ◽  
Bas Teusink ◽  
Hans V. Westerhoff ◽  
Karel van Dam

FEBS Journal ◽  
2014 ◽  
Vol 281 (12) ◽  
pp. 2784-2793 ◽  
Author(s):  
Anna-Karin Gustavsson ◽  
David D. van Niekerk ◽  
Caroline B. Adiels ◽  
Bob Kooi ◽  
Mattias Goksör ◽  
...  

2000 ◽  
Vol 78 (3) ◽  
pp. 1087-1093 ◽  
Author(s):  
Martin Bier ◽  
Barbara M. Bakker ◽  
Hans V. Westerhoff

2010 ◽  
Vol 22 (5) ◽  
pp. 631-638 ◽  
Author(s):  
Hisataka Maruyama ◽  
◽  
Toshio Fukuda ◽  
Fumihito Arai ◽  

Optical adhesion control of hydrogel microtools, made of hydrophilic photo-crosslinkable resin, was developed for on-demand immobilization and measurement of cells on a microfluidic chip. The hydrogel microtool was manipulated by optical tweezers and modified by spiropyran chromospheres, which was a photochromic polymer. We developed on-demand control of uni/bidirectional adhesiveness of the microtool by control of electrolyte concentration in a solution. Photo illumination controls the adhesiveness of the microtools. In case of unidirectional control of adhesiveness, the microtools adhere to glass, other microtools and cells by illumination of ultraviolet (UV) light. Spiropyran chromospheres were used for bidirectional control of adhesiveness to cell. In case of bidirectional control of adhesiveness, the microtools adhere to cells by UV illumination. On the other hand, the microtool detaches from the adhered cells by visible (VIS) light illumination. Electrolyte concentration in the solution controlled these adhesiveness controls. Adherence of the microtool was enough to keep its position on a microfluidic chip. We applied these immobilization methods to measure the local conditions around cells by modifying the microtool with a pH indicator, bromothymol blue (BTB). Local measurements of the ambient pH value of yeast cells were performed by immobilizing the cell on the surface of the pH sensing microtool. Moreover, culture monitoring of a single yeast cell was demonstrated by immobilization to the microtool.


2020 ◽  
Vol 10 (1) ◽  
Author(s):  
André Weber ◽  
Werner Zuschratter ◽  
Marcus J. B. Hauser

AbstractThe transition between synchronized and asynchronous behaviour of immobilized yeast cells of the strain Saccharomyces carlsbergensis was investigated by monitoring the autofluorescence of the coenzyme NADH. In populations of intermediate cell densities the individual cells remained oscillatory, whereas on the level of the cell population both a partially synchronized and an asynchronous state were accessible for experimental studies. In the partially synchronized state, the mean oscillatory frequency was larger than that of the cells in the asynchronous state. This suggests that synchronisation occurred due to entrainment by the cells that oscillated more rapidly. This is typical for synchronisation due to phase advancement. Furthermore, the synchronisation of the frequency of the glycolytic oscillations preceded the synchronisation of their phases. However, the cells did not synchronize completely, as the distribution of the oscillatory frequencies only narrowed but did not collapse to a unique frequency. Cells belonging to spatially denser clusters showed a slightly enhanced local synchronisation during the episode of partial synchronisation. Neither the clusters nor a transition from partially synchronized glycolytic oscillations to travelling glycolytic waves did substantially affect the degree of partial synchronisation. Chimera states, i.e., the coexistence of a synchronized and an asynchronous part of the population, could not be found.


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