Collective and individual glycolytic oscillations in yeast cells encapsulated in alginate microparticles

2015 ◽  
Vol 25 (6) ◽  
pp. 064606 ◽  
Author(s):  
Takashi Amemiya ◽  
Kouhei Obase ◽  
Naoki Hiramatsu ◽  
Kiminori Itoh ◽  
Kenichi Shibata ◽  
...  
Keyword(s):  
Yeast Cells ◽  
Glycolytic Oscillations

Synchronization of Glycolytic Oscillations in Intact Yeast Cells

1993 ◽  
pp. 413-416 ◽  
Author(s):  
Peter Richard ◽  
Bas Teusink ◽  
Hans V. Westerhoff ◽  
Karel van Dam
Keyword(s):  
Yeast Cells ◽  
Glycolytic Oscillations

scholarly journals Allosteric regulation of phosphofructokinase controls the emergence of glycolytic oscillations in isolated yeast cells

FEBS Journal ◽  
2014 ◽  
Vol 281 (12) ◽  
pp. 2784-2793 ◽  
Author(s):  
Anna-Karin Gustavsson ◽  
David D. van Niekerk ◽  
Caroline B. Adiels ◽  
Bob Kooi ◽  
Mattias Goksör ◽  
...  
Keyword(s):  
Allosteric Regulation ◽  
Yeast Cells ◽  
Glycolytic Oscillations

scholarly journals How Yeast Cells Synchronize their Glycolytic Oscillations: A Perturbation Analytic Treatment

2000 ◽  
Vol 78 (3) ◽  
pp. 1087-1093 ◽  
Author(s):  
Martin Bier ◽  
Barbara M. Bakker ◽  
Hans V. Westerhoff
Keyword(s):  
Yeast Cells ◽  
Analytic Treatment ◽  
Glycolytic Oscillations

scholarly journals Partial synchronisation of glycolytic oscillations in yeast cell populations

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
André Weber ◽  
Werner Zuschratter ◽  
Marcus J. B. Hauser
Keyword(s):  
Experimental Studies ◽  
Yeast Cells ◽  
Intermediate Cell ◽  
Oscillatory Frequency ◽  
Glycolytic Oscillations ◽  
Immobilized Yeast Cells ◽  
The Mean ◽  
Cell Densities ◽  
Asynchronous State ◽  
The Individual

AbstractThe transition between synchronized and asynchronous behaviour of immobilized yeast cells of the strain Saccharomyces carlsbergensis was investigated by monitoring the autofluorescence of the coenzyme NADH. In populations of intermediate cell densities the individual cells remained oscillatory, whereas on the level of the cell population both a partially synchronized and an asynchronous state were accessible for experimental studies. In the partially synchronized state, the mean oscillatory frequency was larger than that of the cells in the asynchronous state. This suggests that synchronisation occurred due to entrainment by the cells that oscillated more rapidly. This is typical for synchronisation due to phase advancement. Furthermore, the synchronisation of the frequency of the glycolytic oscillations preceded the synchronisation of their phases. However, the cells did not synchronize completely, as the distribution of the oscillatory frequencies only narrowed but did not collapse to a unique frequency. Cells belonging to spatially denser clusters showed a slightly enhanced local synchronisation during the episode of partial synchronisation. Neither the clusters nor a transition from partially synchronized glycolytic oscillations to travelling glycolytic waves did substantially affect the degree of partial synchronisation. Chimera states, i.e., the coexistence of a synchronized and an asynchronous part of the population, could not be found.

Dynamic regulation of yeast glycolytic oscillations by mitochondrial functions

1991 ◽  
Vol 99 (2) ◽  
pp. 325-334 ◽  
Author(s):  
M.A. Aon ◽  
S. Cortassa ◽  
H.V. Westerhoff ◽  
J.A. Berden ◽  
E Van Spronsen ◽  
...  
Keyword(s):  
Adenine Nucleotide ◽  
Control Level ◽  
Yeast Cells ◽  
Dynamic Regulation ◽  
Model Calculations ◽  
Glycolytic Oscillations ◽  
Mitochondrial Functions ◽  
Sinusoidal Oscillations ◽  
Mitochondrial Staining

The control exerted in vivo by mitochondrial functions on the dynamics of glycolysis was investigated in starved yeast cells that were metabolizing glucose semianaerobically. Glycolytic oscillations were triggered after a pulse of glucose by inhibition of mitochondrial respiration with KCN, myxothiazol and antimycin A or in mutants in the bc1 complex (ubiquinol:cytochrome c reductase) that were largely deficient in respiratory capacity. Inhibition of the adenine nucleotide translocator by preincubation with bongkrekic acid also triggered a train of damped sinusoidal oscillations after glucose addition. The oscillations consisted of cycles of reduction and oxidation of the intracellular pool of nicotinamide nucleotides with periods of 45 s to 1 min and amplitudes of 0.8 mM or lower. Preincubation with the uncoupler carbonyl cyamide p-(trifluoromethoxy)phenylhydrazone (FCCP) annihilated cyanide-induced oscillations of NAD(P)H. Evidence for de-energization of mitochondrial membranes in vivo was obtained by mitochondrial staining with dimethylaminostyryl-methyl-pyridiniumiodine (DASPMI) of starved cells. The low rates of NADH reoxidation shown by respiratory mutants and the FCCP-treated X2180 strain open up the possibility that mitochondrial dehydrogenases also control glycolytic oscillations. Low rates of cytosolic NADH reoxidation induced by pyrazole, an inhibitor of alcohol dehydrogenase, were also associated with the disappearance of glycolytic oscillations. From experimental evidence and model calculations we conclude that the modulation of the levels of cytosolic ATP by mitochondrial functions in turn modulates the approach of the dynamic behavior of glycolysis to an oscillatory domain. The mitochondrial NADH dehydrogenase and the glycolytic steps associated with NADH reoxidation downstream from pyruvate appear to provide another control level of glycolysis dynamics in vivo.

Sustained glycolytic oscillations in individual isolated yeast cells

FEBS Journal ◽  
2012 ◽  
Vol 279 (16) ◽  
pp. 2837-2847 ◽  
Author(s):  
Anna-Karin Gustavsson ◽  
David D. van Niekerk ◽  
Caroline B. Adiels ◽  
Franco B. du Preez ◽  
Mattias Goksör ◽  
...  
Keyword(s):  
Yeast Cells ◽  
Glycolytic Oscillations

scholarly journals Studying Glycolytic Oscillations in Individual Yeast Cells by Combining Fluorescence Microscopy with Microfluidics and Optical Tweezers

2018 ◽  
Vol 82 (1) ◽  
Author(s):  
Anna‐Karin Gustavsson ◽  
Amin A. Banaeiyan ◽  
David D. Niekerk ◽  
Jacky L. Snoep ◽  
Caroline B. Adiels ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Optical Tweezers ◽  
Yeast Cells ◽  
Glycolytic Oscillations

From Synchronised to Desynchronised Glycolytic Oscillations in Individual Yeast Cells

2017 ◽  
pp. 239-254 ◽  
Author(s):  
André Weber ◽  
Yury Prokazov ◽  
Werner Zuschratter ◽  
Marcus J. B. Hauser
Keyword(s):  
Yeast Cells ◽  
Glycolytic Oscillations
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