scholarly journals Bridging the gap between sample collection and laboratory analysis: using dried blood spots to identify human exposure to chemical agents

2016 ◽  
Author(s):  
Elizabeth I. Hamelin ◽  
Thomas A. Blake ◽  
Jonas W. Perez ◽  
Brian S. Crow ◽  
Rebecca L. Shaner ◽  
...  
Keyword(s):  
Human Exposure ◽  
Dried Blood Spots ◽  
Laboratory Analysis ◽  
Sample Collection ◽  
Blood Spots ◽  
Chemical Agents

scholarly journals Assessment of Plasmodium antigens and CRP in dried blood spots with multiplex malaria array

2021 ◽  
Author(s):  
Ihn Kyung Jang ◽  
Sara Aranda ◽  
Rebecca Barney ◽  
Andrew Rashid ◽  
Muhammad Helwany ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Whole Blood ◽  
Dried Blood Spots ◽  
Careful Consideration ◽  
Clinical Samples ◽  
Sample Type ◽  
Sample Collection ◽  
Malaria Surveillance ◽  
Inflammatory Biomarker ◽  
Blood Spots

AbstractDried blood spots (DBS) typically prepared on filter papers are an ideal sample type for malaria surveillance by offering easy and cost-effective methods in terms of sample collection, storage, and transport. The objective of this study was to evaluate the applicability of DBS with a commercial multiplex malaria assay, developed to concurrently measure Plasmodium antigens, histidine-rich protein 2 (HRP2), Plasmodium lactate dehydrogenase (pLDH), and a host inflammatory biomarker, C-reactive protein (CRP), in whole blood. The assay conditions were optimized for DBS, and thermal stability for measurement of Plasmodium antigens and CRP in dried blood were determined. Performance of the multiplex assay on matched DBS and whole blood pellet samples was also evaluated using the clinical samples. The results indicate the acceptable performance in multiplex antigen detection using DBS samples. At cutoff levels for DBS, with a diagnostic specificity with a lower 95% confidence bound > 92%, diagnostic sensitivities against polymerase chain reaction (PCR)–confirmed malaria for HRP2, Pf LDH, Pv LDH, and Pan LDH were 93.5%, 80.4%, 21.3%, and 55.6%, respectively. The half-life of pLDH was significantly less than that of HRP2 in thermal stability studies. Results with DBS samples collected from Peru indicate that the uncontrolled storage conditions of DBS can result in inaccurate reporting for infection with P. falciparum parasites with hrp2/3 deletions. With careful consideration that minimizing the unfavorable DBS storage environment is essential for ensuring integrity of heat-labile Plasmodium antigens, DBS samples can be used as an alternative to liquid whole blood to detect P. falciparum with hrp2/3 deletions in malaria surveillance.

scholarly journals Measurement of Glycated Hemoglobin A1c from Dried Blood by Turbidimetric Immunoassay

2009 ◽  
Vol 3 (5) ◽  
pp. 1203-1206 ◽  
Author(s):  
Ramakrishnan Lakshmy ◽  
Ruby Gupta
Keyword(s):  
Whole Blood ◽  
Large Scale ◽  
Hemoglobin A1c ◽  
Glycated Hemoglobin ◽  
Intraclass Correlation ◽  
Dried Blood Spots ◽  
Sample Collection ◽  
Blood Spots ◽  
Glycated Hemoglobin A1c ◽  
The Impact

Background: Glycated hemoglobin A1c (A1C) is an important marker in the diagnosis and treatment of diabetes. Dried blood measurement of A1C is useful in large scale epidemiological evaluation of A1C, especially to assess the impact of intervention programs. The possibility of using dried blood for measurement of A1C by the immunoturbidimetric method was explored in the present study. Method: Blood was collected from 30 patients, and blood spots were prepared and dried. The dried blood spot samples were kept for different lengths of time at 4°C to assess stability. Glycated hemoglobin was measured in whole blood and dried blood on the day of collection as well as on days 10 and 15 by immunoturbidimetric method. Results: The A1C values of 30 samples analyzed for comparison between whole blood estimation and dried blood ranged from 4.6% to 9.9%. The mean A1C on the day of sample collection was 6.01% ± 1.58% in fresh whole blood samples and 5.94% ± 1.58 % in dried blood spots. A linear and highly correlated relationship was observed between dried blood A1C values and those in whole blood ( r = 0.986 and intraclass correlation value = 0.993). Glycated hemoglobin values on day 10 and day 15 were comparable with the values on day 1 with a shift in mean of just 1% on day 10 and 3.04% on day 15. Conclusion: In conclusion, dried blood can be used for measurement of A1C by immunoturbidimetric method, and further stability of A1C measurement from dried blood for up to 15 days at 4°C makes it an ideal matrix for transportation in developing countries like India.

scholarly journals Stability of Human Immunodeficiency Virus Serological Markers in Samples Collected as HemaSpot and Whatman 903 Dried Blood Spots

2018 ◽  
Vol 56 (10) ◽  
Author(s):  
Mark M. Manak ◽  
Holly R. Hack ◽  
Ashley L. Shutt ◽  
Brook A. Danboise ◽  
Linda L. Jagodzinski ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Western Blot ◽  
Dried Blood Spots ◽  
Early Infection ◽  
Blood Collection ◽  
Signal Recovery ◽  
Sample Collection ◽  
Blood Spots ◽  
Immunodeficiency Virus ◽  
Hiv 1

ABSTRACTDried blood spots (DBS) are frequently used in clinical testing for biosurveillance, infectious disease and confirmatory testing, and clinical trials, particularly for populations in remote areas. The HemaSpot-HF blood collection device (HS) provides an alternative format to the Whatman 903 cards (903) to simplify sample collection and processing. In this study, the performance of the HS was compared to that of the 903 using previously characterized clinical specimens and HIV seroconversion panels known to exhibit markers of early human immunodeficiency virus (HIV) infection. HS and 903 samples were prepared and tested by Bio-Rad GS HIV Combo Ag/Ab enzyme immunoassay (EIA), GS HIV-1/-2 Plus O EIA, GS HIV-1 Western blot, and HIV-1 Geenius assays. Both HS and 903 performed well for up to 6 months at room temperature, but a marked loss of Western blot and low titer antibody signals from early infection samples was observed in samples stored for 180 days at elevated (37 to 45°C) temperatures and high humidity (95%). HemaSpot samples placed in sealed bags with additional desiccant were protected from degradation and showed improved signal recovery relative to that of the 903. HS was easier to use than the 903 and showed higher sensitivity and reproducibility for early infection samples and improved stability.

Dried blood spots (DBS) in doping controls: a complementary matrix for improved in- and out-of-competition sports drug testing strategies

2015 ◽  
Vol 7 (18) ◽  
pp. 7596-7605 ◽  
Author(s):  
Laura Tretzel ◽  
Andreas Thomas ◽  
Hans Geyer ◽  
Valentin Pop ◽  
Wilhelm Schänzer ◽  
...  
Keyword(s):  
Cost Efficiency ◽  
Drug Testing ◽  
State Of The Art ◽  
Analytical Techniques ◽  
Dried Blood Spots ◽  
Sample Collection ◽  
Blood Spots ◽  
Testing Strategies ◽  
In Doping ◽  
Efficiency Test

The use of dried blood spots as a complementary sample matrix combined with state-of-the-art analytical techniques substantially improves doping control efforts, particularly concerning cost-efficiency, test frequency, and sample collection invasiveness.

Effect of sample collection on α-galactosidase A enzyme activity measurements in dried blood spots on filter paper

2009 ◽  
Vol 403 (1-2) ◽  
pp. 159-162 ◽  
Author(s):  
Petra Olivova ◽  
Kristen van der Veen ◽  
Emmaline Cullen ◽  
Michael Rose ◽  
X. Kate Zhang ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Filter Paper ◽  
Dried Blood Spots ◽  
Sample Collection ◽  
Blood Spots ◽  
Activity Measurements

A critical review of the analysis of dried blood spots for characterizing human exposure to inorganic targets using methods based on analytical atomic spectrometry

2020 ◽  
Vol 35 (10) ◽  
pp. 2092-2112 ◽  
Author(s):  
Patrick J. Parsons ◽  
Aubrey L. Galusha ◽  
Yuxia Cui ◽  
Elaine M. Faustman ◽  
Jill C. Falman ◽  
...  
Keyword(s):  
Critical Review ◽  
Human Exposure ◽  
Dried Blood Spots ◽  
Atomic Spectrometry ◽  
Blood Spots ◽  
Analytical Atomic Spectrometry

Several researchers have reported using dried blood spots to measure inorganic targets but with varying degrees of success.

scholarly journals Serological detection of SARS-CoV-2 IgG using a commercially available enzyme immunoassays on dried blood spots collected from patients

2021 ◽  
Author(s):  
Gregory J Walker ◽  
Rebecca Davis ◽  
Zin Naing ◽  
Brad McEntee ◽  
Yonghui Lu ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Dried Blood Spots ◽  
Infants And Children ◽  
Serological Testing ◽  
Sample Collection ◽  
Serum Samples ◽  
Blood Spots ◽  
Specimen Collection ◽  
Paired Serum ◽  
Heel Prick

Serological testing for SARS-CoV-2 antibodies provides important research and diagnostic information relating to COVID-19 immune response and surveillance. A major challenge when addressing protection post- infection or vaccination is the difficulty of specimen collection from infants and children. Dried blood spots (DBS) collected by finger prick or heel prick are a minimally invasive sample collection alternative previously used to detect antibodies to other viruses. In this study we evaluated DBS for the detection of SARS-CoV-2 antibodies on three commercially available enzyme (EIA) and chemiluminescent (CLIA) immunoassays by analysing paired DBS and serum samples collected from 54 subjects. We demonstrate that testing of DBS samples was highly sensitive and specific, and quantitative results strongly correlated with those of paired serum. These results suggest that DBS derived blood is a viable alternative to plasma or serum for use in EIAs and CLIAs, and has particular utility as a minimally invasive collection tool for COVID-19 serological testing of infants and children.

scholarly journals Detection of ctDNA from dried blood spots after DNA size selection

2019 ◽  
Author(s):  
Katrin Heider ◽  
Jonathan C. M. Wan ◽  
James Hall ◽  
Samantha Boyle ◽  
Irena Hudecova ◽  
...  
Keyword(s):  
Copy Number ◽  
Fragment Size ◽  
Dried Blood Spots ◽  
Dried Blood Spot ◽  
Copy Number Alterations ◽  
Sample Collection ◽  
Cell Free Dna ◽  
Blood Spots ◽  
Free Dna ◽  
Blood Spot

AbstractRecent advances in the research and clinical applications of circulating tumour DNA (ctDNA) is limited by practical considerations of sample collection. Whole genome sequencing (WGS) is increasingly used for analysis of ctDNA, identifying copy-number alterations, fragment size patterns, and other genomic features. We hypothesised that low-depth WGS data may be generated from minute amounts of cell-free DNA, and that fragment-size selection may be effective to remove contaminating genomic DNA (gDNA) from small volumes of blood. There are practical advantages to using dried blood spots as these are easier to collect, facilitate serial sampling, and support novel study designs in prospective human studies, animal models and expand the utilisation of archival samples by the removal of gDNA in small volumes. We therefore developed a protocol for the isolation and analysis of cell-free DNA from dried blood spots. Analysing a dried blood spot of 50μL frozen whole blood from a patient with melanoma, we identified ctDNA based on tumour-specific somatic copy-number alterations, and found a fragment size profile similar to that observed in plasma DNA processed by traditional methods. We extended this approach to detect tumour-derived cell-free DNA in a dried blood spot from a mouse xenograft model and were able to identify ctDNA from the originally grafted ascites. Together, our data suggests that ctDNA can be detected and monitored in dried blood spots. This will enable new approaches for sample collection from patients andin vivomodels.

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