Exploring the nanoscale dynamics of biomolecules with optical microcavities (Conference Presentation)

Author(s):  
Frank Vollmer
Author(s):  
Yanqin Zhai ◽  
Peng Luo ◽  
Michihiro Nagao ◽  
Kenji Nakajima ◽  
Tatsuya Kikuchi ◽  
...  

2-propanol was investigated, in both the liquid and supercooled states, as a model system to study how hydrogen bonds affect the structural relaxation and the dynamics of mesoscale structures, of...


2013 ◽  
Vol 103 (21) ◽  
pp. 211116 ◽  
Author(s):  
Kevin D. Heylman ◽  
Randall H. Goldsmith

Author(s):  
Haiyong Quan ◽  
Zhixiong (James) Guo

Laser energy transfer and molecule-radiation interaction in optical microcavity devices are characterized. The device is operated at whispering-gallery modes, and consists of a microcavity and a micro-waveguide coupled by a sub-micrometer air-gap. Emphases are placed on the influences of microcavity size and waveguide compatibility on the energy transfer and storage capability, on the interactions of foreign molecules with the evanescent radiation field surrounding a resonant microcavity. An optimal gap is found for the considered device configuration where maximum energy storage is achieved. This optimal gap is dependent on the resonance mode as well as the morphology. The Q factor increases exponentially with increasing gap and saturates as the gap approaches the optical wavelength. The influence of molecules attachment is demonstrated and the potential in molecular detection is discussed.


2018 ◽  
Vol 218 (1) ◽  
pp. 83-96 ◽  
Author(s):  
Lena K. Schroeder ◽  
Andrew E.S. Barentine ◽  
Holly Merta ◽  
Sarah Schweighofer ◽  
Yongdeng Zhang ◽  
...  

The endoplasmic reticulum (ER) is composed of interconnected membrane sheets and tubules. Superresolution microscopy recently revealed densely packed, rapidly moving ER tubules mistaken for sheets by conventional light microscopy, highlighting the importance of revisiting classical views of ER structure with high spatiotemporal resolution in living cells. In this study, we use live-cell stimulated emission depletion (STED) microscopy to survey the architecture of the ER at 50-nm resolution. We determine the nanoscale dimensions of ER tubules and sheets for the first time in living cells. We demonstrate that ER sheets contain highly dynamic, subdiffraction-sized holes, which we call nanoholes, that coexist with uniform sheet regions. Reticulon family members localize to curved edges of holes within sheets and are required for their formation. The luminal tether Climp63 and microtubule cytoskeleton modulate their nanoscale dynamics and organization. Thus, by providing the first quantitative analysis of ER membrane structure and dynamics at the nanoscale, our work reveals that the ER in living cells is not limited to uniform sheets and tubules; instead, we suggest the ER contains a continuum of membrane structures that includes dynamic nanoholes in sheets as well as clustered tubules.


2019 ◽  
Vol 16 (3) ◽  
pp. 211-219 ◽  
Author(s):  
Bernard Gelloz ◽  
Kouichiro Murata ◽  
Toshiyuki Ohta ◽  
Mher Ghulinyan ◽  
Lorenzo Pavesi ◽  
...  

Author(s):  
Qi-Fan Yang ◽  
Xu Yi ◽  
Ki Youl Yang ◽  
Kerry J. Vahala

Optik ◽  
2018 ◽  
Vol 165 ◽  
pp. 168-173 ◽  
Author(s):  
Francis Segovia-Chaves ◽  
Herbert Vinck-Posada

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