scholarly journals Interferometric and nonlinear-optical spectral-imaging techniques for outer space and live cells

2015 ◽  
Author(s):  
Kazuyoshi Itoh
Keyword(s):  
Nonlinear Optical ◽  
Imaging Techniques ◽  
Outer Space ◽  
Spectral Imaging ◽  
Live Cells

scholarly journals 3D super-resolved imaging in live cells using sub-diffractive plasmonic localization of hybrid nanopillar arrays

Nanophotonics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 2847-2859
Author(s):  
Soojung Kim ◽  
Hyerin Song ◽  
Heesang Ahn ◽  
Seung Won Jun ◽  
Seungchul Kim ◽  
...  
Keyword(s):  
Signal To Noise Ratio ◽  
Imaging Techniques ◽  
Optical Loss ◽  
Super Resolution ◽  
Living Cells ◽  
Live Cells ◽  
Complex Biological System ◽  
Observation Volume ◽  
Resolution Imaging ◽  
Nanopillar Arrays

AbstractAnalysing dynamics of a single biomolecule using high-resolution imaging techniques has been had significant attentions to understand complex biological system. Among the many approaches, vertical nanopillar arrays in contact with the inside of cells have been reported as a one of useful imaging applications since an observation volume can be confined down to few-tens nanometre theoretically. However, the nanopillars experimentally are not able to obtain super-resolution imaging because their evanescent waves generate a high optical loss and a low signal-to-noise ratio. Also, conventional nanopillars have a limitation to yield 3D information because they do not concern field localization in z-axis. Here, we developed novel hybrid nanopillar arrays (HNPs) that consist of SiO2 nanopillars terminated with gold nanodisks, allowing extreme light localization. The electromagnetic field profiles of HNPs are obtained through simulations and imaging resolution of cell membrane and biomolecules in living cells are tested using one-photon and 3D multiphoton fluorescence microscopy, respectively. Consequently, HNPs present approximately 25 times enhanced intensity compared to controls and obtained an axial and lateral resolution of 110 and 210 nm of the intensities of fluorophores conjugated with biomolecules transported in living cells. These structures can be a great platform to analyse complex intracellular environment.

scholarly journals AIE-Driven Fluorescent Polysaccharide Polymersome as Enzyme-responsive FRET Nanoprobe to Study the Real-time Delivery Aspects in Live-Cells

2021 ◽  
Author(s):  
Nilesh Umakant Deshpande ◽  
Mishika Virmani ◽  
Manickam Jayakannan
Keyword(s):  
Energy Transfer ◽  
Confocal Microscopy ◽  
Real Time ◽  
Fluorescence Resonance Energy Transfer ◽  
Imaging Techniques ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Live Cells ◽  
Intracellular Enzyme ◽  
Bio Imaging

We report aggregation induced emission (AIE) driven polysaccharide polymersome as fluorescence resonance energy transfer (FRET) nanoprobes to study their intracellular enzyme-responsive delivery by real-time live-cell confocal microscopy bio-imaging techniques. AIE...

scholarly journals Multimodal Nonlinear Optical Imaging of Live Cells Using Plasmon-Coupled DNA-Mediated Gold Nanoprism Assembly

2016 ◽  
Vol 120 (8) ◽  
pp. 4546-4555 ◽  
Author(s):  
Sudarson Sekhar Sinha ◽  
Stacy Jones ◽  
Teresa Demeritte ◽  
Suhash Reddy Chavva ◽  
Yongliang Shi ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Nonlinear Optical ◽  
Live Cells ◽  
Nonlinear Optical Imaging

scholarly journals Recovering spectral images from compressive measurements using designed coded apertures and Matrix Completion Theory

2017 ◽  
Vol 21 (2) ◽  
Author(s):  
Tatiana Gelvez ◽  
Hoover Rueda ◽  
Henry Arguello
Keyword(s):  
Imaging Techniques ◽  
Matrix Completion ◽  
Spectral Imaging ◽  
Spatial Location ◽  
Low Rank ◽  
Coded Aperture ◽  
Reconstruction Algorithms ◽  
Spectral Image ◽  
The Matrix ◽  
Spectral Imager

<p>Spectral imaging aims to capture and process a 3-dimensional spectral image with a large amount of spectral information for each spatial location. Compressive spectral imaging techniques (CSI) increases the sensing speed and reduces the amount of collected data compared to traditional spectral imaging methods. The coded aperture snapshot spectral imager (CASSI) is an optical architecture to sense a spectral image in a single 2D coded projection by applying CSI. Typically, the 3D scene is recovered by solving an L1-based optimization problem that assumes the scene is sparse in some known orthonormal basis. In contrast, the matrix completion technique (MC) allows to recover the scene without such prior knowledge. The MC reconstruction algorithms rely on a low-rank structure of the scene. Moreover, the CASSI system uses coded aperture patterns that determine the quality of the estimated scene. Therefore, this paper proposes the design of an optimal coded aperture set for the MC methodology. The designed set is attained by maximizing the distance between the translucent elements in the coded aperture. Visualization of the recovered spectral signals and simulations over different databases show average improvement when the designed coded set is used between 1-3 dBs compared to the complementary coded aperture set, and between 3-9 dBs compared to the conventional random coded aperture set.</p>

Modigliani's studio practice revealed by MA‐XRF and non‐invasive spectral imaging techniques

2020 ◽  
Author(s):  
Anaïs Genty‐Vincent ◽  
Eric Laval ◽  
Marie‐Amélie Senot ◽  
Michel Menu
Keyword(s):  
Imaging Techniques ◽  
Spectral Imaging ◽  
Non Invasive ◽  
Studio Practice

scholarly journals Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Hela Benaissa ◽  
Karim Ounoughi ◽  
Isabelle Aujard ◽  
Evelyne Fischer ◽  
Rosette Goïame ◽  
...  
Keyword(s):  
Spectral Properties ◽  
Rational Design ◽  
Imaging Techniques ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Super Resolution ◽  
Visible Spectrum ◽  
Live Cells ◽  
Fluorescent Reporters ◽  
Entire Visible Spectrum

AbstractBiocompatible fluorescent reporters with spectral properties spanning the entire visible spectrum are indispensable tools for imaging the biochemistry of living cells and organisms in real time. Here, we report the engineering of a fluorescent chemogenetic reporter with tunable optical and spectral properties. A collection of fluorogenic chromophores with various electronic properties enables to generate bimolecular fluorescent assemblies that cover the visible spectrum from blue to red using a single protein tag engineered and optimized by directed evolution and rational design. The ability to tune the fluorescence color and properties through simple molecular modulation provides a broad experimental versatility for imaging proteins in live cells, including neurons, and in multicellular organisms, and opens avenues for optimizing Förster resonance energy transfer (FRET) biosensors in live cells. The ability to tune the spectral properties and fluorescence performance enables furthermore to match the specifications and requirements of advanced super-resolution imaging techniques.

scholarly journals Characterization of the eyespot and hematochrome-like granules of Euglena gracilis by scan-free absorbance spectral imaging A(x, y, λ) for quantification of carotenoids within the live cells

2018 ◽  
Author(s):  
Kyohei Yamashita ◽  
Takafumi Yagi ◽  
Takumi Isono ◽  
Yusuke Nishiyama ◽  
Masafumi Hashimoto ◽  
...  
Keyword(s):  
Euglena Gracilis ◽  
Absorption Maximum ◽  
Spectral Imaging ◽  
Basic Research ◽  
Live Cells ◽  
Carotenoid Content ◽  
Characteristic Absorption ◽  
Non Invasive ◽  
Characteristic Absorption Peak

Euglena gracilis is an edible photosynthetic single-cell alga that can synthesize carotenoids. It is highly demanded to establish the technology to select and grow individual cells capable of synthesizing more carotenoids because it contributes to safe and inexpensive production of carotenoids. In the cells of E. gracilis, carotenoids are mainly contained in chloroplasts and eyespots, and typical carotenoids have a characteristic absorption maximum in common. E. gracilis also has an organelle resembling hematochrome, which has an appearance similar to the eyespot and the absorption band spectrally overlapping that of the carotenoid although reportedly it does not contain carotenoids. To discriminate the eyespot and hematochrome-like granules and to investigate the intracellular distribution of carotenoids, scan-free, non-invasive, absorbance spectral imaging A(x, y, λ) microscopy of single live cells was applied. It was demonstrated that this technique is a powerful tool not only for basic research on intracellular structural analysis but also for identifying difference in carotenoid content in individual cells applicable to screening of carotenoid-rich cells. By this technique, it was confirmed that carotenoids exist in chloroplasts and eyespots, and a number of characteristic absorption spectra of pigments observed specific to the eyespot or hematochrome-like granules were identified. In addition, it was found that hematochrome-like granules have a characteristic absorption peak at 620 nm as well as at 676 nm, suggesting that its origin is a component of chloroplast including Chlorophyll a.

scholarly journals Nonlinear optical imaging techniques (NLO) for painting investigation

2017 ◽  
Author(s):  
Alice Dal Fovo ◽  
◽  
Raffaella Fontana ◽  
Jana Striova ◽  
Enrico Pampaloni ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Nonlinear Optical ◽  
Imaging Techniques ◽  
Nonlinear Optical Imaging
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