Three dimensional time lapse imaging of live cell mitochondria with photothermal optical lock-in optical coherence microscopy (Conference Presentation)

2016 ◽  
Author(s):  
Miguel Sison ◽  
Sabyasachi Chakrabortty ◽  
Jerome Extermann ◽  
Amir Nahas ◽  
Christophe Pache ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Time Lapse ◽  
Live Cell ◽  
Optical Coherence ◽  
Optical Coherence Microscopy ◽  
Lock In ◽  
Time Lapse Imaging

scholarly journals Fast three-dimensional imaging of gold nanoparticles in living cells with photothermal optical lock-in Optical Coherence Microscopy

2012 ◽  
Vol 20 (19) ◽  
pp. 21385 ◽  
Author(s):  
Christophe Pache ◽  
Noelia L. Bocchio ◽  
Arno Bouwens ◽  
Martin Villiger ◽  
Corinne Berclaz ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Three Dimensional ◽  
Living Cells ◽  
Optical Coherence ◽  
Optical Coherence Microscopy ◽  
Three Dimensional Imaging ◽  
Lock In

scholarly journals Analysis of Ca2+ response of osteocyte network by three-dimensional time-lapse imaging in living bone

2017 ◽  
Vol 36 (5) ◽  
pp. 519-528 ◽  
Author(s):  
Tomoyo Tanaka ◽  
Mitsuhiro Hoshijima ◽  
Junko Sunaga ◽  
Takashi Nishida ◽  
Mana Hashimoto ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Time Lapse ◽  
Living Bone ◽  
Time Lapse Imaging

scholarly journals Live-cell time-lapse imaging and single-cell tracking of in vitro cultured neural stem cells – Tools for analyzing dynamics of cell cycle, migration, and lineage selection

Methods ◽  
2018 ◽  
Vol 133 ◽  
pp. 81-90 ◽  
Author(s):  
Katja M. Piltti ◽  
Brian J. Cummings ◽  
Krystal Carta ◽  
Ayla Manughian-Peter ◽  
Colleen L. Worne ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Single Cell ◽  
Cell Tracking ◽  
Time Lapse ◽  
Live Cell ◽  
Time Lapse Imaging

scholarly journals Label-free three-dimensional imaging of Caenorhabditis elegans with visible optical coherence microscopy

PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0181676 ◽  
Author(s):  
Séverine Coquoz ◽  
Paul J. Marchand ◽  
Arno Bouwens ◽  
Laurent Mouchiroud ◽  
Vincenzo Sorrentino ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Three Dimensional ◽  
Optical Coherence ◽  
Label Free ◽  
Optical Coherence Microscopy ◽  
Three Dimensional Imaging

In vivo three-dimensional imaging of plants with optical coherence microscopy

2002 ◽  
Vol 208 (3) ◽  
pp. 177-189 ◽  
Author(s):  
A. Reeves ◽  
R. L. Parsons ◽  
J. W. Hettinger ◽  
J. I. Medford
Keyword(s):  
Three Dimensional ◽  
Optical Coherence ◽  
Optical Coherence Microscopy ◽  
Three Dimensional Imaging

scholarly journals Time‐lapse imaging assay using the BioStation CT : A sensitive drug‐screening method for three‐dimensional cell culture

2015 ◽  
Vol 106 (6) ◽  
pp. 757-765 ◽  
Author(s):  
Ruriko Sakamoto ◽  
M. Mamunur Rahman ◽  
Manami Shimomura ◽  
Manabu Itoh ◽  
Tetsuya Nakatsura
Keyword(s):  
Cell Culture ◽  
Drug Screening ◽  
Screening Method ◽  
Three Dimensional ◽  
Time Lapse ◽  
Time Lapse Imaging ◽  
Dimensional Cell

Toxicity Evaluation of Two Dental Composites: Three-Dimensional Confocal Laser Scanning Microscopy Time-Lapse Imaging of Cell Behavior

2013 ◽  
Vol 19 (3) ◽  
pp. 596-607 ◽  
Author(s):  
Ghania Nina Attik ◽  
Nelly Pradelle-Plasse ◽  
Doris Campos ◽  
Pierre Colon ◽  
Brigitte Grosgogeat
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Cell Metabolism ◽  
Three Dimensional ◽  
Time Lapse ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Dental Composites ◽  
Confocal Laser ◽  
Time Lapse Imaging

AbstractThe purpose of this study was to investigate thein vitrobiocompatibility of two dental composites (namely A and B) with similar chemical composition used for direct restoration using three-dimensional confocal laser scanning microscopy (CLSM) time-lapse imaging. Time-lapse imaging was performed on cultured human HGF-1 fibroblast-like cells after staining using Live/Dead®. Image analysis showed a higher mortality rate in the presence of composite A than composite B. The viability rate decreased in a time-dependent manner during the 5 h of exposure. Morphological alterations were associated with toxic effects; cells were enlarged and more rounded in the presence of composite A as shown by F-actin and cell nuclei staining. Resazurin assay was used to confirm the active potential of composites in cell metabolism; results showed severe cytotoxic effects in the presence of both no light-curing composites after 24 h of direct contact. However, extracts of polymerized composites induced a moderate decrease in cell metabolism after the same incubation period. Composite B was significantly better tolerated than composite A at all investigated end points and all time points. The finding confirmed that the used CLSM method was sufficiently sensitive to differentiate the biocompatibility behavior of two composites based on similar methacrylate monomers.

scholarly journals Immunotargeting of Nanocrystals by SpyCatcher Conjugation of Engineered Antibodies

2021 ◽  
Author(s):  
Cassio Pedroso ◽  
Victor Mann ◽  
Kathrin Zuberbühler ◽  
Markus-Frederik Bohn ◽  
Jessica Yu ◽  
...  
Keyword(s):  
Cell Surface ◽  
Specific Binding ◽  
Time Lapse ◽  
Live Cell ◽  
Confocal Imaging ◽  
Cellular Targets ◽  
Type Plasminogen Activator ◽  
Urokinase Type Plasminogen Activator ◽  
Isopeptide Bonds ◽  
Time Lapse Imaging

Inorganic nanocrystals such as quantum dots (QDs) and upconverting nanoparticles (UCNPs) are uniquely suited for quanti-tative live-cell imaging and are typically functionalized with ligands to study specific receptors or cellular targets. Antibod-ies (Ab) are among the most useful targeting reagents owing to their high affinities and specificities, but common nanocrys-tal labeling methods may orient Ab incorrectly, be reversible or denaturing, or lead to Ab-NP complexes too large for some applications. Here, we show that SpyCatcher proteins, which bind and spontaneously form covalent isopeptide bonds with cognate SpyTag peptides, can conjugate engineered Ab to nanoparticle surfaces with control over stability, orientation, and stoichiometry. Compact SpyCatcher-functionalized QDs and UCNPs may be labeled with short-chain variable fragment Ab (scFv) engineered to bind urokinase-type plasminogen activator receptors (uPAR) that are overexpressed in many human can-cers. Confocal imaging of anti-uPAR scFv-QD conjugates shows the Ab mediates specific binding and internalization by breast cancer cells expressing uPAR. Time-lapse imaging of photostable scFv-UCNP conjugates show that Ab binding caus-es uPAR internalization with a ∼20-minute half-life on the cell surface, and uPAR is internalized to endolysosomal com-partments distinct from general membrane stains and without significant recycling to the cell surface. The controlled and stable conjugation of engineered Ab to NPs enables targeting of diverse receptors for live-cell study of their distribution, trafficking, and physiology.

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