scholarly journals Time‐lapse imaging assay using the BioStation CT : A sensitive drug‐screening method for three‐dimensional cell culture

2015 ◽  
Vol 106 (6) ◽  
pp. 757-765 ◽  
Author(s):  
Ruriko Sakamoto ◽  
M. Mamunur Rahman ◽  
Manami Shimomura ◽  
Manabu Itoh ◽  
Tetsuya Nakatsura
Keyword(s):  
Cell Culture ◽  
Drug Screening ◽  
Screening Method ◽  
Three Dimensional ◽  
Time Lapse ◽  
Time Lapse Imaging ◽  
Dimensional Cell

A novel three-dimensional cell culture method enhances antiviral drug screening in primary human cells

2018 ◽  
Vol 150 ◽  
pp. 20-29 ◽  
Author(s):  
Robert Koban ◽  
Markus Neumann ◽  
Aila Daugs ◽  
Oliver Bloch ◽  
Andreas Nitsche ◽  
...  
Keyword(s):  
Cell Culture ◽  
Drug Screening ◽  
Antiviral Drug ◽  
Three Dimensional ◽  
Culture Method ◽  
Human Cells ◽  
Dimensional Cell ◽  
Primary Human Cells

scholarly journals Three-dimensional cell culture models for anticancer drug screening: Worth the effort?

2017 ◽  
Vol 233 (4) ◽  
pp. 2993-3003 ◽  
Author(s):  
Eddy-Tim Verjans ◽  
Jordi Doijen ◽  
Walter Luyten ◽  
Bart Landuyt ◽  
Liliane Schoofs
Keyword(s):  
Cell Culture ◽  
Anticancer Drug ◽  
Drug Screening ◽  
Three Dimensional ◽  
Culture Models ◽  
Cell Culture Models ◽  
Dimensional Cell

scholarly journals Factors to consider when interrogating 3D culture models with plate readers or automated microscopes

2021 ◽  
Author(s):  
Terry Riss ◽  
O. Joseph Trask
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Fluorescent Imaging ◽  
Culture Models ◽  
Assay Methods ◽  
Diffusion Rates ◽  
Cell Culture Models ◽  
Dimensional Cell ◽  
Cells Cultured

AbstractAlong with the increased use of more physiologically relevant three-dimensional cell culture models comes the responsibility of researchers to validate new assay methods that measure events in structures that are physically larger and more complex compared to monolayers of cells. It should not be assumed that assays designed using monolayers of cells will work for cells cultured as larger three-dimensional masses. The size and barriers for penetration of molecules through the layers of cells result in a different microenvironment for the cells in the outer layer compared to the center of three-dimensional structures. Diffusion rates for nutrients and oxygen may limit metabolic activity which is often measured as a marker for cell viability. For assays that lyse cells, the penetration of reagents to achieve uniform cell lysis must be considered. For live cell fluorescent imaging assays, the diffusion of fluorescent probes and penetration of photons of light for probe excitation and fluorescent emission must be considered. This review will provide an overview of factors to consider when implementing assays to interrogate three dimensional cell culture models.

scholarly journals Analysis of Ca2+ response of osteocyte network by three-dimensional time-lapse imaging in living bone

2017 ◽  
Vol 36 (5) ◽  
pp. 519-528 ◽  
Author(s):  
Tomoyo Tanaka ◽  
Mitsuhiro Hoshijima ◽  
Junko Sunaga ◽  
Takashi Nishida ◽  
Mana Hashimoto ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Time Lapse ◽  
Living Bone ◽  
Time Lapse Imaging

A novel fluffy hydroxylapatite fiber scaffold with deep interconnected pores designed for three-dimensional cell culture

2014 ◽  
Vol 2 (1) ◽  
pp. 129-136 ◽  
Author(s):  
Lin Jin ◽  
Zhang-Qi Feng ◽  
Ting Wang ◽  
Zhuozhuo Ren ◽  
Shuangshuang Ma ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Interconnected Pores ◽  
Dimensional Cell

Ho:YAG laser: intervertebral disk cell interaction using three-dimensional cell culture system

2000 ◽  
Author(s):  
Masato Sato ◽  
Miya Ishihara ◽  
Tsunenori Arai ◽  
Takashi Asazuma ◽  
Toshiyuki Kikuchi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Interaction ◽  
Intervertebral Disk ◽  
Cell Culture System ◽  
Ho:Yag Laser ◽  
Dimensional Cell

Preparation of Porous Organically-Modified Silicate Hybrids for Cell Culture Matrix

2007 ◽  
Vol 330-332 ◽  
pp. 1177-1180 ◽  
Author(s):  
Kanji Tsuru ◽  
Satoshi Hayakawa ◽  
Yuki Shirosaki ◽  
T. Okayama ◽  
K. Kataoka ◽  
...  
Keyword(s):  
Cell Culture ◽  
Mammalian Cells ◽  
Pore Diameter ◽  
Sol Gel ◽  
Three Dimensional ◽  
Culture Methods ◽  
Organically Modified ◽  
Dimensional Cell

Porous & rubbery organic-inorganic hybrids were synthesized from tetraethoxysilane (TEOS) and polydimethylsiloxane (PDMS) through a sol-gel route using sieved sucrose granules as a porogen. The porous hybrids with a high content of PDMS behaved like polymer sponge. The porosity was over 90% irrespective of the hybrid composition and the pore diameter ranged from 100 to 500 μm. In the three-dimensional cell culture, mammalian cells were well cultured in the porous hybrids. The present results indicate that the hybrids may be a promising scaffold for developing such functional culture methods.

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