scholarly journals OptoDyCE: Automated system for high-throughput all-optical dynamic cardiac electrophysiology

2016 ◽  
Author(s):  
Aleksandra Klimas ◽  
Jinzhu Yu ◽  
Christina M. Ambrosi ◽  
John C. Williams ◽  
Harold Bien ◽  
...  
Keyword(s):  
High Throughput ◽  
Cardiac Electrophysiology ◽  
Automated System ◽  
All Optical

scholarly journals OptoDyCE: Automated System for High-Throughput All-Optical Dynamic Cardiac Electrophysiology

2015 ◽  
Author(s):  
Aleksandra Klimas ◽  
Jinzhu Yu ◽  
Christina M. Ambrosi ◽  
John C. Williams ◽  
Harold Bien ◽  
...  
Keyword(s):  
High Throughput ◽  
Cardiac Electrophysiology ◽  
Drug Testing ◽  
Dynamic Testing ◽  
Cellular Level ◽  
Automated System ◽  
Human Cardiomyocytes ◽  
Tissue Constructs ◽  
Time Space ◽  
All Optical

The improvement of preclinical cardiotoxicity testing, the discovery of new ion channel-targeted drugs, and the phenotyping and use of stem-cell-derived cardiomyocytes and other biologics all necessitate high-throughput (HT), cellular level electrophysiological interrogation tools. Optical techniques for actuation and sensing provide instant parallelism, enabling contactless dynamic HT testing of cells and small-tissue constructs, not affordable by other means. Here, we consider, computationally and experimentally, the limits of all-optical electrophysiology when applied to drug testing, then implement and validate OptoDyCE, a fully automated system for all-optical cardiac electrophysiology. We validate optical actuation by virally introducing optogenetic drivers in (rat and human) cardiomyocytes or through the modular use of dedicated light-sensitive somatic "spark" cells. We show that this automated all-optical approach provides high-throughput means of cellular interrogation, i.e. allows for dynamic testing of >600 multicellular samples or compounds per hour, and yields high-content information about the action of a drug over time, space and doses.

scholarly journals OptoDyCE as an automated system for high-throughput all-optical dynamic cardiac electrophysiology

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Aleksandra Klimas ◽  
Christina M. Ambrosi ◽  
Jinzhu Yu ◽  
John C. Williams ◽  
Harold Bien ◽  
...  
Keyword(s):  
High Throughput ◽  
Cardiac Electrophysiology ◽  
Automated System ◽  
All Optical

High-Throughput Evaluation of CYP1A1 and 2B1 Induction in Rat Liver Slices Using a Semi-Automated System

2009 ◽  
Vol 3 (2) ◽  
pp. 108-114
Author(s):  
Jairam Palamanda ◽  
Xinjie Lin ◽  
Pramila Kumari ◽  
Amin Nomeir
Keyword(s):  
Rat Liver ◽  
High Throughput ◽  
Automated System ◽  
Liver Slices

scholarly journals Clinical evaluation of the Procleix SARS-CoV-2 assay, a sensitive, high-throughput test that runs on an automated system

2021 ◽  
pp. 115560
Author(s):  
Silvia Sauleda ◽  
Lourdes Palacios ◽  
Vanessa Brès ◽  
Maria Piñana ◽  
Lorena Alonso-Hernandez ◽  
...  
Keyword(s):  
Clinical Evaluation ◽  
High Throughput ◽  
Automated System

scholarly journals All-Optical Electrophysiology for High-Throughput Functional Characterization of a Human iPSC-Derived Motor Neuron Model of ALS

2018 ◽  
Vol 10 (6) ◽  
pp. 1991-2004 ◽  
Author(s):  
Evangelos Kiskinis ◽  
Joel M. Kralj ◽  
Peng Zou ◽  
Eli N. Weinstein ◽  
Hongkang Zhang ◽  
...  
Keyword(s):  
Motor Neuron ◽  
High Throughput ◽  
Neuron Model ◽  
Functional Characterization ◽  
All Optical ◽  
Human Ipsc

scholarly journals Gnare: Automated System For High-Throughput Genome Analysis With Grid Computational Backend

2005 ◽  
Vol 19 (4-5) ◽  
pp. 361-369 ◽  
Author(s):  
Dinanath Sulakhe ◽  
Alex Rodriguez ◽  
Mark D'Souza ◽  
Michael Wilde ◽  
Veronika Nefedova ◽  
...  
Keyword(s):  
High Throughput ◽  
Genome Analysis ◽  
Automated System

scholarly journals Upgrading a microplate reader for photobiology and all-optical experiments

2015 ◽  
Vol 14 (2) ◽  
pp. 270-279 ◽  
Author(s):  
Florian Richter ◽  
Ulrike S. Scheib ◽  
Jennifer Mehlhorn ◽  
Roman Schubert ◽  
Jonas Wietek ◽  
...  
Keyword(s):  
Open Source ◽  
High Throughput ◽  
Optical Components ◽  
All Optical ◽  
Microplate Reader

Using open-source electronics and optical components, we add programmable illumination capabilities to a multimode microplate reader, thus enabling photobiological and all-optical experiments at high throughput, reproducibility and fidelity.

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