Surface enhanced Raman spectroscopy as a point-of-care diagnostic for infection in wound effluent

2016 ◽  
Author(s):  
Meron Ghebremedhin ◽  
Shubha Yesupriya ◽  
Nicole J. Crane
Keyword(s):  
Raman Spectroscopy ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Wound Effluent

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

scholarly journals Recent Advancement in the Surface-Enhanced Raman Spectroscopy-Based Biosensors for Infectious Disease Diagnosis

2019 ◽  
Vol 9 (7) ◽  
pp. 1448 ◽  
Author(s):  
Logan Hamm ◽  
Amira Gee ◽  
A. Swarnapali De Silva Indrasekara
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Neglected Tropical Diseases ◽  
Point Of Care ◽  
Disease Diagnosis ◽  
Surface Enhanced Raman Spectroscopy ◽  
Biomarker Detection ◽  
Disease Biomarker ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Diagnosis is the key component in disease elimination to improve global health. However, there is a tremendous need for diagnostic innovation for neglected tropical diseases that largely consist of mosquito-borne infections and bacterial infections. Early diagnosis of these infectious diseases is critical but challenging because the biomarkers are present at low concentrations, demanding bioanalytical techniques that can deliver high sensitivity with ensured specificity. Owing to the plasmonic nanomaterials-enabled high detection sensitivities, even up to single molecules, surface-enhanced Raman spectroscopy (SERS) has gained attention as an optical analytical tool for early disease biomarker detection. In this mini-review, we highlight the SERS-based assay development tailored to detect key types of biomarkers for mosquito-borne and bacterial infections. We discuss in detail the variations of SERS-based techniques that have developed to afford qualitative and quantitative disease biomarker detection in a more accurate, affordable, and field-transferable manner. Current and emerging challenges in the advancement of SERS-based technologies from the proof-of-concept phase to the point-of-care phase are also briefly discussed.

LOC-SERS: towards point-of-care diagnostic of methotrexate

2014 ◽  
Vol 6 (12) ◽  
pp. 3943-3947 ◽  
Author(s):  
I. J. Hidi ◽  
A. Mühlig ◽  
M. Jahn ◽  
F. Liebold ◽  
D. Cialla ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Therapeutic Range ◽  
Point Of Care ◽  
Lab On A Chip ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Methotrexate (MTX), an antifolate antibiotic, is detected using a lab-on-a-chip device via surface enhanced Raman spectroscopy (LOC-SERS) in the therapeutic range of 10 μm to 0.1 μm.

Development of an electrochemical surface-enhanced Raman spectroscopy (EC-SERS) fabric-based plasmonic sensor for point-of-care diagnostics

The Analyst ◽  
2018 ◽  
Vol 143 (17) ◽  
pp. 4128-4135 ◽  
Author(s):  
Shruti D. Bindesri ◽  
Dalal S. Alhatab ◽  
Christa L. Brosseau
Keyword(s):  
Raman Spectroscopy ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
Plasmonic Sensor ◽  
Point Of Care Diagnostics ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Sensing

A plasmonic fabric-based electrode has been developed for wearable EC-SERS sensing for point-of-care applications.

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

scholarly journals Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

Nanophotonics ◽  
2017 ◽  
Vol 6 (4) ◽  
pp. 681-701 ◽  
Author(s):  
Haley Marks ◽  
Monika Schechinger ◽  
Javier Garza ◽  
Andrea Locke ◽  
Gerard Coté
Keyword(s):  
Raman Spectroscopy ◽  
Point Of Care ◽  
Low Cost ◽  
Diagnostic Testing ◽  
Surface Enhanced Raman Spectroscopy ◽  
World Health ◽  
Great Promise ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

AbstractPoint-of-care (POC) device development is a growing field that aims to develop low-cost, rapid, sensitive in-vitro diagnostic testing platforms that are portable, self-contained, and can be used anywhere – from modern clinics to remote and low resource areas. In this review, surface enhanced Raman spectroscopy (SERS) is discussed as a solution to facilitating the translation of bioanalytical sensing to the POC. The potential for SERS to meet the widely accepted “ASSURED” (Affordable, Sensitive, Specific, User-friendly, Rapid, Equipment-free, and Deliverable) criterion provided by the World Health Organization is discussed based on recent advances in SERS in vitro assay development. As SERS provides attractive characteristics for multiplexed sensing at low concentration limits with a high degree of specificity, it holds great promise for enhancing current efforts in rapid diagnostic testing. In outlining the progression of SERS techniques over the past years combined with recent developments in smart nanomaterials, high-throughput microfluidics, and low-cost paper diagnostics, an extensive number of new possibilities show potential for translating SERS biosensors to the POC.

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