3D imaging of the cleared intact murine colon with light sheet microscopy

2016 ◽  
Author(s):  
B. Zufiria ◽  
D. I. Bocancea ◽  
M. V. Gómez-Gaviro ◽  
J. J. Vaquero ◽  
M. Desco ◽  
...  
Keyword(s):  
3D Imaging ◽  
Light Sheet ◽  
Light Sheet Microscopy ◽  
Murine Colon

Rapid high resolution 3D imaging of expanded biological specimens with lattice light sheet microscopy

Methods ◽  
2020 ◽  
Vol 174 ◽  
pp. 11-19 ◽  
Author(s):  
Yun-Chi Tsai ◽  
Wei-Chun Tang ◽  
Christine Siok Lan Low ◽  
Yen-Ting Liu ◽  
Jyun-Sian Wu ◽  
...  
Keyword(s):  
High Resolution ◽  
3D Imaging ◽  
Light Sheet ◽  
Light Sheet Microscopy

3D imaging of cleared human skin biopsies using light-sheet microscopy: A new way to visualize in-depth skin structure

2018 ◽  
Vol 24 (2) ◽  
pp. 294-303 ◽  
Author(s):  
S. Abadie ◽  
C. Jardet ◽  
J. Colombelli ◽  
B. Chaput ◽  
A. David ◽  
...  
Keyword(s):  
Human Skin ◽  
3D Imaging ◽  
Light Sheet ◽  
Skin Structure ◽  
Light Sheet Microscopy ◽  
Skin Biopsies

scholarly journals 3D Imaging of BABB Cleared Whole-Mount Organs Using Light Sheet Microscopy

2018 ◽  
Vol 24 (S1) ◽  
pp. 1390-1391
Author(s):  
Kingsley A. Boateng ◽  
Austin Cyphersmith ◽  
Glenn A. Fried ◽  
Barghav S. Sivaguru ◽  
Xiaochen Lu ◽  
...  
Keyword(s):  
3D Imaging ◽  
Light Sheet ◽  
Light Sheet Microscopy ◽  
Whole Mount

scholarly journals Label-free and Multimodal Second Harmonic Generation Light Sheet Microscopy

2020 ◽  
Author(s):  
Niall Hanrahan ◽  
Simon I. R. Lane ◽  
Peter Johnson ◽  
Konstantinos Bourdakos ◽  
Christopher Brereton ◽  
...  
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Biological Samples ◽  
3D Imaging ◽  
Second Harmonic ◽  
Three Dimensional ◽  
Light Sheet ◽  
Optical Methods ◽  
Label Free ◽  
Light Sheet Microscopy

AbstractLight sheet microscopy (LSM) has emerged as one of most profound three dimensional (3D) imaging tools in the life sciences over the last decade. However, LSM is currently performed with fluorescence detection on one- or multi-photon excitation. Label-free LSM imaging approaches have been rather limited. Second Harmonic Generation (SHG) imaging is a label-free technique that has enabled detailed investigation of collagenous structures, including its distribution and remodelling in cancers and respiratory tissue, and how these link to disease. SHG is generally regarded as having only forward- and back-scattering components, apparently precluding the orthogonal detection geometry used in Light Sheet Microscopy. In this work we demonstrate SHG imaging on a light sheet microscope (SHG-LSM) using a rotated Airy beam configuration that demonstrates a powerful new approach to direct, without any further processing or deconvolution, 3D imaging of harmonophores such as collagen in biological samples. We provide unambiguous identification of SHG signals on the LSM through its wavelength and polarisation sensitivity. In a multimodal LSM setup we demonstrate that SHG and two-photon signals can be acquired on multiple types of different biological samples. We further show that SHG-LSM is sensitive to changes in collagen synthesis within lung fibroblast 3D cell cultures. This work expands on the existing optical methods available for use with light sheet microscopy, adding a further label-free imaging technique which can be combined with other detection modalities to realise a powerful multi-modal microscope for 3D bioimaging.

scholarly journals 3D-Imaging of Whole Neuronal and Vascular Networks of the Human Dental Pulp via CLARITY and Light Sheet Microscopy

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Cristiane Miranda França ◽  
Rachelle Riggers ◽  
John L. Muschler ◽  
Matthias Widbiller ◽  
Peter Manning Lococo ◽  
...  
Keyword(s):  
Dental Pulp ◽  
3D Imaging ◽  
Light Sheet ◽  
Vascular Networks ◽  
Light Sheet Microscopy ◽  
Human Dental Pulp

scholarly journals Fast 3D imaging of giant unilamellar vesicles using reflected light‐sheet microscopy with single molecule sensitivity

2021 ◽  
Author(s):  
Anita Jannasch ◽  
Sven A. Szilagyi ◽  
Moritz Burmeister ◽  
Q. Tyrell Davis ◽  
Gero L. Hermsdorf ◽  
...  
Keyword(s):  
Single Molecule ◽  
3D Imaging ◽  
Light Sheet ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Light Sheet Microscopy ◽  
Reflected Light

scholarly journals Multiplane Encoded Light-Sheet Microscopy for Enhanced 3D Imaging

ACS Photonics ◽  
2021 ◽  
Author(s):  
Alessandro Zunino ◽  
Francesco Garzella ◽  
Alberta Trianni ◽  
Peter Saggau ◽  
Paolo Bianchini ◽  
...  
Keyword(s):  
3D Imaging ◽  
Light Sheet ◽  
Light Sheet Microscopy

scholarly journals 3D Model Characterization by 2D and 3D Imaging in t(14;18)-Positive B-NHL: Perspectives for In Vitro Drug Screens in Follicular Lymphoma

Cancers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1490
Author(s):  
Fabien Gava ◽  
Carla Faria ◽  
Pauline Gravelle ◽  
Juan G. Valero ◽  
Cèlia Dobaño-López ◽  
...  
Keyword(s):  
Follicular Lymphoma ◽  
3D Imaging ◽  
Cell Model ◽  
Drug Effects ◽  
Light Sheet ◽  
Morphological Properties ◽  
Response To Treatment ◽  
Light Sheet Microscopy ◽  
2D Imaging

Follicular lymphoma (FL) is an indolent B cell lymphoproliferative disorder of transformed follicular center B cells, which accounts for 20–30 percent of all non-Hodgkin lymphoma (NHL) cases. Great advances have been made to identify the most relevant targets for precision therapy. However, no relevant models for in vitro studies have been developed or characterized in depth. To this purpose, we generated a 3D cell model from t(14;18)-positive B-NHL cell lines cultured in ultra-low attachment 96-well plates. Morphological features and cell growth behavior were evaluated by classical microscopy (2D imaging) and response to treatment with different drugs was evaluated by a high-content analysis system to determine the robustness of the model. We show that the ultra-low attachment (ULA) method allows the development of regular, spherical and viable ULA-multicellular aggregates of lymphoma cells (MALC). However, discrepancies in the results obtained after 2D imaging analyses on drug-treated ULA-MALC prompted us to develop 3D imaging and specific analyses. We show by using light sheet microscopy and specifically developed 3D imaging algorithms that 3D imaging and dedicated analyses are necessary to characterize morphological properties of 3D models and drug effects. This study proposes a new method, but also imaging tools and informatic solutions, developed for FL necessary for future preclinical studies.

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