Wide-field synovial fluid imaging using polarized lens-free on-chip microscopy for point-of-care diagnostics of gout (Conference Presentation)

2016 ◽  
Author(s):  
Yibo Zhang ◽  
Seung Yoon Lee ◽  
Yun Zhang ◽  
Daniel Furst ◽  
John Fitzgerald ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Point Of Care ◽  
Wide Field ◽  
Point Of Care Diagnostics ◽  
On Chip ◽  
Fluid Imaging

scholarly journals High-Precision Lensless Microscope on a Chip Based on In-Line Holographic Imaging

Sensors ◽  
2021 ◽  
Vol 21 (3) ◽  
pp. 720
Author(s):  
Xiwei Huang ◽  
Yangbo Li ◽  
Xuefeng Xu ◽  
Renjie Wang ◽  
Jiangfan Yao ◽  
...  
Keyword(s):  
High Precision ◽  
Point Of Care ◽  
Reference Image ◽  
Wide Field ◽  
Point Of Care Testing ◽  
Phase Recovery ◽  
Holographic Imaging ◽  
Micro Particles ◽  
Shadow Imaging ◽  
On Chip

The lensless on-chip microscope is an emerging technology in the recent decade that can realize the imaging and analysis of biological samples with a wide field-of-view without huge optical devices and any lenses. Because of its small size, low cost, and being easy to hold and operate, it can be used as an alternative tool for large microscopes in resource-poor or remote areas, which is of great significance for the diagnosis, treatment, and prevention of diseases. To improve the low-resolution characteristics of the existing lensless shadow imaging systems and to meet the high-resolution needs of point-of-care testing, here, we propose a high-precision on-chip microscope based on in-line holographic technology. We demonstrated the ability of the iterative phase recovery algorithm to recover sample information and evaluated it with image quality evaluation algorithms with or without reference. The results showed that the resolution of the holographic image after iterative phase recovery is 1.41 times that of traditional shadow imaging. Moreover, we used machine learning tools to identify and count the mixed samples of mouse ascites tumor cells and micro-particles that were iterative phase recovered. The results showed that the on-chip cell counter had high-precision counting characteristics as compared with manual counting of the microscope reference image. Therefore, the proposed high-precision lensless microscope on a chip based on in-line holographic imaging provides one promising solution for future point-of-care testing (POCT).

An integrated CMOS quantitative-polymerase-chain-reaction lab-on-chip for point-of-care diagnostics

Lab on a Chip ◽  
2014 ◽  
Vol 14 (20) ◽  
pp. 4076-4084 ◽  
Author(s):  
Haig Norian ◽  
Ryan M. Field ◽  
Ioannis Kymissis ◽  
Kenneth L. Shepard
Keyword(s):  
Integrated Circuit ◽  
Point Of Care ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Lab On Chip ◽  
Cmos Integrated Circuit ◽  
Point Of Care Diagnostics ◽  
Droplet Transport ◽  
Polymerase Chain ◽  
On Chip

qPCR demonstrated on the surface of a CMOS integrated circuit using embedded heaters, temperature sensors, photodiodes, and electrowetting-based droplet transport mechanism.

scholarly journals On-Chip PCR Based Plasmonic Microfluidic Platform: Ultrafast Point-of-Care Diagnostics of SARS-CoV-2

2021 ◽  
Vol 3 (1) ◽  
pp. 5-11
Author(s):  
Megha Agrawal ◽  
Keyword(s):  
Small Volume ◽  
Point Of Care ◽  
Rapid Screening ◽  
Viral Diseases ◽  
Chain Reaction ◽  
Point Of Care Diagnostics ◽  
Screening And Identification ◽  
Polymerase Chain ◽  
Diagnostic Applications ◽  
On Chip

It is critically important to have rapid screening and identification of contagious viral diseases such as the current COVID-19 pandemic that is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Rapid and accurate diagnostic is essential for preventing worldwide spread of virus and ensuring in-time care for patients during the fast spread of pandemic diseases. Nanobiotechnology enabled tools have allowed to develop advanced polymerase chain reaction (PCR) based diagnostics of contagious viral diseases. To this end, microfluidic on-chip PCR platforms have shown huge promise for highly efficient, rapid and small-volume bioassay for point-of-care (POC) diagnostic applications in mitigating the challenges of SARS-CoV-2. Here, we discuss latest advances in ultrafast, real-time, and on-chip nanoplasmonic PCR for rapid and quantitative molecular diagnostics at POC level.

scholarly journals Trends in photonic lab-on-chip interferometric biosensors for point-of-care diagnostics

2016 ◽  
Vol 8 (48) ◽  
pp. 8380-8394 ◽  
Author(s):  
A. B. González-Guerrero ◽  
J. Maldonado ◽  
S. Herranz ◽  
L. M. Lechuga
Keyword(s):  
Environmental Protection ◽  
Point Of Care ◽  
Lab On Chip ◽  
Universal Healthcare ◽  
Point Of Care Diagnostics ◽  
On Chip

Portable point-of care (POC) devices forin vitrodiagnostics will be a milestone for the achievement of universal healthcare and environmental protection.

scholarly journals COVID-19 Point-of-Care Diagnostics: Present and Future

ACS Nano ◽  
2021 ◽  
Author(s):  
Enrique Valera ◽  
Aaron Jankelow ◽  
Jongwon Lim ◽  
Victoria Kindratenko ◽  
Anurup Ganguli ◽  
...  
Keyword(s):  
Point Of Care ◽  
Point Of Care Diagnostics

scholarly journals 145 Point of care lung ultrasound in patient triage: integration of ultrasound into a streaming pathway for COVID-19

2020 ◽  
Vol 37 (12) ◽  
pp. 839.1-839
Author(s):  
Dominic Craver ◽  
Aminah Ahmad ◽  
Anna Colclough
Keyword(s):  
Point Of Care ◽  
District General Hospital ◽  
Training Programme ◽  
Service Evaluation ◽  
University Hospital ◽  
Junior Doctors ◽  
Point Of Care Ultrasound ◽  
Face To Face ◽  
Point Of Care Diagnostics ◽  
Audit Data

Aims/Objectives/BackgroundRapid risk stratification of patients is vital for Emergency Department (ED) streaming during the COVID-19 pandemic. Ideally, patients should be split into red (suspected/confirmed COVID-19) and green (non COVID-19) zones in order to minimise the risk of patient-to-patient and patient-to-staff transmission. A robust yet rapid streaming system combining clinician impression with point-of-care diagnostics is therefore necessary.Point of care ultrasound (POCUS) findings in COVID-19 have been shown to correlate well with computed tomography (CT) findings, and it therefore has value as a front-door diagnostic tool. At University Hospital Lewisham (a district general hospital in south London), we recognised the value of early POCUS and its potential for use in patient streaming.Methods/DesignWe developed a training programme, ‘POCUS for COVID’ and subsequently integrated POCUS into streaming of our ED patients. The training involved Zoom lectures, a face to face practical, a 10 scan sign off process followed by a final triggered assessment. Patient outcomes were reviewed in conjunction with their scan reports.Results/ConclusionsCurrently, we have 21 ED junior doctors performing ultrasound scans independently, and all patients presenting to our department are scanned either in triage or in the ambulance. A combination of clinical judgement and scan findings are used to stream the patient to an appropriate area.Service evaluation with analysis of audit data has found our streaming to be 94% sensitive and 79% specific as an indicator of COVID 19. Further analysis is ongoing.Here we present both the structure of our training programme and our integrated streaming pathway along with preliminary analysis results.

scholarly journals Point of Care Diagnostics in the Age of COVID-19

Diagnostics ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 9
Author(s):  
Meysam Rezaei ◽  
Sajad Razavi Bazaz ◽  
Sareh Zhand ◽  
Nima Sayyadi ◽  
Dayong Jin ◽  
...  
Keyword(s):  
Point Of Care ◽  
Diagnostic Methods ◽  
Global Public Health ◽  
Current Standard ◽  
Healthcare Facilities ◽  
Major Threat ◽  
Point Of Care Diagnostics ◽  
Recent Outbreak ◽  
Control Virus ◽  
Biosafety Level

The recent outbreak of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated serious respiratory disease, coronavirus disease 2019 (COVID-19), poses a major threat to global public health. Owing to the lack of vaccine and effective treatments, many countries have been overwhelmed with an exponential spread of the virus and surge in the number of confirmed COVID-19 cases. Current standard diagnostic methods are inadequate for widespread testing as they suffer from prolonged turn-around times (>12 h) and mostly rely on high-biosafety-level laboratories and well-trained technicians. Point-of-care (POC) tests have the potential to vastly improve healthcare in several ways, ranging from enabling earlier detection and easier monitoring of disease to reaching remote populations. In recent years, the field of POC diagnostics has improved markedly with the advent of micro- and nanotechnologies. Due to the COVID-19 pandemic, POC technologies have been rapidly innovated to address key limitations faced in existing standard diagnostic methods. This review summarizes and compares the latest available POC immunoassay, nucleic acid-based and clustered regularly interspaced short palindromic repeats- (CRISPR)-mediated tests for SARS-CoV-2 detection that we anticipate aiding healthcare facilities to control virus infection and prevent subsequent spread.

scholarly journals Pocket MUSE: an affordable, versatile and high-performance fluorescence microscope using a smartphone

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yehe Liu ◽  
Andrew M. Rollins ◽  
Richard M. Levenson ◽  
Farzad Fereidouni ◽  
Michael W. Jenkins
Keyword(s):  
High Performance ◽  
Point Of Care ◽  
Low Cost ◽  
Consumer Electronics ◽  
Practical Implementation ◽  
The Novel ◽  
Point Of Care Diagnostics ◽  
Optical Module ◽  
Optical Configuration ◽  
User Friendly

AbstractSmartphone microscopes can be useful tools for a broad range of imaging applications. This manuscript demonstrates the first practical implementation of Microscopy with Ultraviolet Surface Excitation (MUSE) in a compact smartphone microscope called Pocket MUSE, resulting in a remarkably effective design. Fabricated with parts from consumer electronics that are readily available at low cost, the small optical module attaches directly over the rear lens in a smartphone. It enables high-quality multichannel fluorescence microscopy with submicron resolution over a 10× equivalent field of view. In addition to the novel optical configuration, Pocket MUSE is compatible with a series of simple, portable, and user-friendly sample preparation strategies that can be directly implemented for various microscopy applications for point-of-care diagnostics, at-home health monitoring, plant biology, STEM education, environmental studies, etc.

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