Three-dimensional fluorescence imaging by stage-scanning oblique plane microscopy (Conference Presentation)

2016 ◽  
Author(s):  
Vincent Maioli ◽  
Frederik Görlitz ◽  
Sean Warren ◽  
Sunil Kumar ◽  
Paul M. W. French ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Three Dimensional ◽  
Stage Scanning

Lab-on-a-Chip System for Developing and Fluorescence Imaging a Three-Dimensional Model of Pancreatic Islets Under Flow Conditions

2021 ◽  
Vol MA2021-01 (55) ◽  
pp. 1396-1396
Author(s):  
Zbigniew Brzozka ◽  
Patrycja Sokołowska ◽  
Kamil Zukowski ◽  
Justyna Janikiewicz ◽  
Ekzbieta Jastrzebska ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Fluorescence Imaging ◽  
Three Dimensional ◽  
Lab On A Chip ◽  
Dimensional Model ◽  
Flow Conditions ◽  
Three Dimensional Model

scholarly journals Constriction and Dnm1p Recruitment Are Distinct Processes in Mitochondrial Fission

2003 ◽  
Vol 14 (5) ◽  
pp. 1953-1963 ◽  
Author(s):  
Aster Legesse-Miller ◽  
Ramiro H. Massol ◽  
Tom Kirchhausen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fluorescence Imaging ◽  
Hot Spots ◽  
Mitochondrial Membrane ◽  
Mitochondrial Fission ◽  
Three Dimensional ◽  
Time Lapse ◽  
Outer Mitochondrial Membrane ◽  
Mitochondrial Matrix ◽  
Different Shapes

Mitochondria undergo cycles of fusion and fission crucial for organelle homeostasis. Fission is regulated partially by recruitment of the large GTPase Dnm1p to the outer mitochondrial membrane. Using three-dimensional time-lapse fluorescence imaging of Saccharomyces cerevisiae cells, we found that Dnm1p-EGFP appears and disappears at “hot spots” along mitochondrial tubes. It forms patches that convert rapidly into different shapes regardless of whether mitochondrial fission ensues or not. Moreover, the thickness of the mitochondrial matrix displays frequent temporal fluctuations apparently unrelated to fission or to recruitment of Dnm1p-EGFP. These results suggest that mitochondrial fission requires coordination of at least two distinct processes.

scholarly journals Three Dimensional Fluorescence Imaging Using Multiple Light-Sheet Microscopy

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e96551 ◽  
Author(s):  
Kavya Mohan ◽  
Subhajit B. Purnapatra ◽  
Partha Pratim Mondal
Keyword(s):  
Fluorescence Imaging ◽  
Three Dimensional ◽  
Light Sheet ◽  
Light Sheet Microscopy

scholarly journals Three-Dimensional, Tomographic Super-Resolution Fluorescence Imaging of Serially Sectioned Thick Samples

PLoS ONE ◽  
2012 ◽  
Vol 7 (5) ◽  
pp. e38098 ◽  
Author(s):  
Siddharth Nanguneri ◽  
Benjamin Flottmann ◽  
Heinz Horstmann ◽  
Mike Heilemann ◽  
Thomas Kuner
Keyword(s):  
Fluorescence Imaging ◽  
Three Dimensional ◽  
Super Resolution

scholarly journals Three-dimensional volume fluorescence-imaging of vascular plasticity in adipose tissues

2018 ◽  
Vol 14 ◽  
pp. 71-81 ◽  
Author(s):  
Ying Cao ◽  
Huanhuan Wang ◽  
Qi Wang ◽  
Xiangli Han ◽  
Wenwen Zeng
Keyword(s):  
Fluorescence Imaging ◽  
Three Dimensional ◽  
Adipose Tissues

Tissue-culture light sheet fluorescence microscopy (TC-LSFM) allows long-term imaging of three-dimensional cell cultures under controlled conditions

2014 ◽  
Vol 6 (10) ◽  
pp. 988-998 ◽  
Author(s):  
Francesco Pampaloni ◽  
Ulrich Berge ◽  
Anastasios Marmaras ◽  
Peter Horvath ◽  
Ruth Kroschewski ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Fluorescence Microscopy ◽  
Fluorescence Imaging ◽  
Cell Cultures ◽  
Three Dimensional ◽  
Light Sheet ◽  
Controlled Conditions ◽  
Light Sheet Fluorescence Microscopy ◽  
Dimensional Cell

This novel system for the long-term fluorescence imaging of live three-dimensional cultures provides minimal photodamage, control of temperature, CO2, pH, and media flow.

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