Tissue-culture light sheet fluorescence microscopy (TC-LSFM) allows long-term imaging of three-dimensional cell cultures under controlled conditions

2014 ◽  
Vol 6 (10) ◽  
pp. 988-998 ◽  
Author(s):  
Francesco Pampaloni ◽  
Ulrich Berge ◽  
Anastasios Marmaras ◽  
Peter Horvath ◽  
Ruth Kroschewski ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Fluorescence Microscopy ◽  
Fluorescence Imaging ◽  
Cell Cultures ◽  
Three Dimensional ◽  
Light Sheet ◽  
Controlled Conditions ◽  
Light Sheet Fluorescence Microscopy ◽  
Dimensional Cell

This novel system for the long-term fluorescence imaging of live three-dimensional cultures provides minimal photodamage, control of temperature, CO2, pH, and media flow.

Three-dimensional imaging on a chip using optofluidics light-sheet fluorescence microscopy

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Erick Vargas Ordaz ◽  
Sergey Gorelick ◽  
Harrison York ◽  
Bonan Liu ◽  
Michelle L. Halls ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescence Imaging ◽  
Three Dimensional ◽  
Light Sheet ◽  
Three Dimensional Imaging ◽  
Cellular Organelle ◽  
Resolution Imaging ◽  
Light Sheet Fluorescence Microscopy

Volumetric, sub-micron to micron level resolution imaging is necessary to assay phenotypes or characteristics at the sub-cellular/organelle scale. However, three-dimensional fluorescence imaging of cells is typically low throughput or compromises...

scholarly journals Light-Sheet Fluorescence Microscopy with Scanning Non-diffracting Beams

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hosein Kafian ◽  
Meelad Lalenejad ◽  
Sahar Moradi-Mehr ◽  
Shiva Akbari Birgani ◽  
Daryoush Abdollahpour
Keyword(s):  
Fluorescence Microscopy ◽  
Spatial Coherence ◽  
Three Dimensional ◽  
Light Sheet ◽  
Tissue Imaging ◽  
Wide Field ◽  
Self Healing ◽  
Main Challenge ◽  
Cancer Tumor ◽  
Light Sheet Fluorescence Microscopy

Abstract Light-sheet fluorescence microscopy (LSFM) has now become a unique tool in different fields ranging from three-dimensional (3D) tissue imaging to real-time functional imaging of neuronal activities. Nevertheless, obtaining high-quality artifact-free images from large, dense and inhomogeneous samples is the main challenge of the method that still needs to be adequately addressed. Here, we demonstrate significant enhancement of LSFM image qualities by using scanning non-diffracting illuminating beams, both through experimental and numerical investigations. The effect of static and scanning illumination with several beams are analyzed and compared, and it is shown that scanning 2D Airy light-sheet is minimally affected by the inhomogeneities in the samples, and provides higher contrasts and uniform resolution over a wide field-of-view, due to its reduced spatial coherence, self-healing feature and longer penetration depth. Further, the capabilities of the illumination scheme is utilized for both single-and double-wavelength 3D imaging of large and dense mammospheres of cancer tumor cells as complex inhomogeneous biological samples.

scholarly journals Monitoring of Apoptosis in 3D Cell Cultures by FRET and Light Sheet Fluorescence Microscopy

2015 ◽  
Vol 16 (12) ◽  
pp. 5375-5385 ◽  
Author(s):  
Petra Weber ◽  
Sarah Schickinger ◽  
Michael Wagner ◽  
Brigitte Angres ◽  
Thomas Bruns ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Cultures ◽  
Light Sheet ◽  
3D Cell Cultures ◽  
Light Sheet Fluorescence Microscopy

Limited-view light sheet fluorescence microscopy for three dimensional volume imaging

2015 ◽  
Vol 107 (26) ◽  
pp. 263701 ◽  
Author(s):  
C. K. Rasmi ◽  
Kavya Mohan ◽  
M. Madhangi ◽  
K. Rajan ◽  
U. Nongthomba ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Three Dimensional ◽  
Light Sheet ◽  
Volume Imaging ◽  
Light Sheet Fluorescence Microscopy

scholarly journals Light-Sheet Fluorescence Microscopy with Scanning Non-diffracting Beams

2019 ◽  
Author(s):  
Hosein Kafian ◽  
Meelad Lalenejad ◽  
Sahar Moradi-Mehr ◽  
Shiva Akbari Birgani ◽  
Daryoush Abdollahpour
Keyword(s):  
Fluorescence Microscopy ◽  
Spatial Coherence ◽  
Three Dimensional ◽  
Light Sheet ◽  
Tissue Imaging ◽  
Wide Field ◽  
Self Healing ◽  
Main Challenge ◽  
Cancer Tumor ◽  
Light Sheet Fluorescence Microscopy

AbstractLight-sheet fluorescence microscopy (LSFM) has now become a unique technique in different fields ranging from three-dimensional (3D) tissue imaging to real-time functional imaging of neuronal activities. Nevertheless, obtaining high-quality artifact-free images from large, dense and inhomogeneous samples is the main challenge of the method that still needs to be adequately addressed. Here, we demonstrate significant enhancement of LSFM image qualities by using scanning non-diffracting illuminating beams, both through experimental and numerical investigations. The effect of static and scanning illumination with several beams are analyzed and compared, and it is shown that scanning 2D Airy light sheet is minimally affected by the artifacts, and provide higher contrasts and uniform resolution over a wide field-of-view, due to its reduced spatial coherence, self-healing feature and higher penetration depth. Further, the capabilities of the illumination scheme is utilized for both single and double wavelength 3D imaging of a large and dense mammospheres of cancer tumor cells as complex inhomogeneous biological samples.

scholarly journals Monitoring microbial communities using light sheet fluorescence microscopy

2017 ◽  
Author(s):  
Raghuveer Parthasarathy
Keyword(s):  
Fluorescence Microscopy ◽  
Microbial Communities ◽  
Three Dimensional ◽  
Light Sheet ◽  
Rapid Acquisition ◽  
History Of ◽  
The Many ◽  
And Function ◽  
Microbial Systems ◽  
Light Sheet Fluorescence Microscopy

Microbes often live in dense, dynamic, multi-species communities whose architecture and function are intimately intertwined. Imaging these complex, three-dimensional ensembles presents considerable technical challenges, however. In this review, I describe light sheet fluorescence microscopy, a technique that enables rapid acquisition of three-dimensional images over large fields of view and over long durations, and I highlight recent applications of this method to microbial systems that include artificial closed ecosystems, bacterial biofilms, and gut microbiota. I comment also on the history of light sheet imaging and the many variants of the method. Light sheet techniques have tremendous potential for illuminating the workings of microbial communities, a potential that is just beginning to be realized.

scholarly journals Monitoring microbial communities using light sheet fluorescence microscopy

2017 ◽  
Author(s):  
Raghuveer Parthasarathy
Keyword(s):  
Fluorescence Microscopy ◽  
Microbial Communities ◽  
Three Dimensional ◽  
Light Sheet ◽  
Rapid Acquisition ◽  
History Of ◽  
The Many ◽  
And Function ◽  
Microbial Systems ◽  
Light Sheet Fluorescence Microscopy

Microbes often live in dense, dynamic, multi-species communities whose architecture and function are intimately intertwined. Imaging these complex, three-dimensional ensembles presents considerable technical challenges, however. In this review, I describe light sheet fluorescence microscopy, a technique that enables rapid acquisition of three-dimensional images over large fields of view and over long durations, and I highlight recent applications of this method to microbial systems that include artificial closed ecosystems, bacterial biofilms, and gut microbiota. I comment also on the history of light sheet imaging and the many variants of the method. Light sheet techniques have tremendous potential for illuminating the workings of microbial communities, a potential that is just beginning to be realized.

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