Comparison of sensor structures for the signal amplification of surface plasmon resonance immunoassay using enzyme precipitation

2015 ◽  
Author(s):  
Chih-Tsung Yang ◽  
Benjamin Thierry
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Amplification

scholarly journals Surface plasmon resonance biosensor for exosome detection based on reformative tyramine signal amplification activated by molecular aptamer beacon

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Wenqin Chen ◽  
Zhiyang Li ◽  
Wenqian Cheng ◽  
Tao Wu ◽  
Jia Li ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Amplification ◽  
Lipid Membrane ◽  
Breast Cancer Patients ◽  
Her2 Positive ◽  
Chip Surface ◽  
Spr Biosensor ◽  
Aptamer Beacon

AbstractHuman epidermal growth factor receptor 2 (HER2)-positive exosomes play an extremely important role in the diagnosis and treatment options of breast cancers. Herein, based on the reformative tyramine signal amplification (TSA) enabled by molecular aptamer beacon (MAB) conversion, a label-free surface plasmon resonance (SPR) biosensor was proposed for highly sensitive and specific detection of HER2-positive exosomes. The exosomes were captured by the HER2 aptamer region of MAB immobilized on the chip surface, which enabled the exposure of the G-quadruplex DNA (G4 DNA) that could form peroxidase-like G4-hemin. In turn, the formed G4-hemin catalyzed the deposition of plentiful tyramine-coated gold nanoparticles (AuNPs-Ty) on the exosome membrane with the help of H2O2, generating a significantly enhanced SPR signal. In the reformative TSA system, the horseradish peroxidase (HRP) as a major component was replaced with nonenzymic G4-hemin, bypassing the defects of natural enzymes. Moreover, the dual-recognition of the surface proteins and lipid membrane of the desired exosomes endowed the sensing strategy with high specificity without the interruption of free proteins. As a result, this developed SPR biosensor exhibited a wide linear range from 1.0 × 104 to 1.0 × 107 particles/mL. Importantly, this strategy was able to accurately distinguish HER2-positive breast cancer patients from healthy individuals, exhibiting great potential clinical application. Graphical Abstract

Signal amplification on planar and gel-type sensor surfaces in surface plasmon resonance-based detection of prostate-specific antigen

2004 ◽  
Vol 333 (1) ◽  
pp. 165-173 ◽  
Author(s):  
Geert A.J. Besselink ◽  
Rob P.H. Kooyman ◽  
Peter J.H.J. van Os ◽  
Gerard H.M. Engbers ◽  
Richard B.M. Schasfoort
Keyword(s):  
Surface Plasmon Resonance ◽  
Prostate Specific Antigen ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Amplification ◽  
Specific Antigen

A highly sensitive surface plasmon resonance sensor for the detection of DNA and cancer cells by a target-triggered multiple signal amplification strategy

2014 ◽  
Vol 50 (73) ◽  
pp. 10718-10721 ◽  
Author(s):  
Peng He ◽  
Wenping Qiao ◽  
Lijun Liu ◽  
Shusheng Zhang
Keyword(s):  
Surface Plasmon Resonance ◽  
Cancer Cells ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Amplification ◽  
Surface Plasmon Resonance Sensor ◽  
Multiple Signal ◽  
Highly Sensitive ◽  
Resonance Sensor ◽  
Amplification Strategy

A SPR bioassay was developed for the detection of DNA and Ramos cells by combining the target-triggered isothermal exponential amplification with MNP-based RCA.

scholarly journals Signal Amplification by Enzymatic Reaction in an Immunosensor Based on Localized Surface Plasmon Resonance (LSPR)

Sensors ◽  
2010 ◽  
Vol 10 (3) ◽  
pp. 2045-2053 ◽  
Author(s):  
Tae-Han Lee ◽  
Seung-Woo Lee ◽  
Ji-Ae Jung ◽  
Junhyoung Ahn ◽  
Min-Gon Kim ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Amplification ◽  
Localized Surface Plasmon Resonance ◽  
Enzymatic Reaction ◽  
Localized Surface Plasmon
Sign in / Sign up

Export Citation Format

Share Document