High-sensitive surface plasmon resonance microRNA biosensor based on streptavidin functionalized gold nanorods-assisted signal amplification

2017 ◽  
Vol 954 ◽  
pp. 114-120 ◽  
Author(s):  
Kaihong Hao ◽  
Yu He ◽  
Huiting Lu ◽  
Shaotao Pu ◽  
Yingnan Zhang ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Gold Nanorods ◽  
Plasmon Resonance ◽  
Signal Amplification

Gold nanorods with finely tunable longitudinal surface plasmon resonance as SERS substrates

2011 ◽  
Vol 22 (26) ◽  
pp. 265705 ◽  
Author(s):  
S L Smitha ◽  
K G Gopchandran ◽  
T R Ravindran ◽  
V S Prasad
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Gold Nanorods ◽  
Plasmon Resonance ◽  
Sers Substrates

scholarly journals Surface plasmon resonance biosensor for exosome detection based on reformative tyramine signal amplification activated by molecular aptamer beacon

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Wenqin Chen ◽  
Zhiyang Li ◽  
Wenqian Cheng ◽  
Tao Wu ◽  
Jia Li ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Amplification ◽  
Lipid Membrane ◽  
Breast Cancer Patients ◽  
Her2 Positive ◽  
Chip Surface ◽  
Spr Biosensor ◽  
Aptamer Beacon

AbstractHuman epidermal growth factor receptor 2 (HER2)-positive exosomes play an extremely important role in the diagnosis and treatment options of breast cancers. Herein, based on the reformative tyramine signal amplification (TSA) enabled by molecular aptamer beacon (MAB) conversion, a label-free surface plasmon resonance (SPR) biosensor was proposed for highly sensitive and specific detection of HER2-positive exosomes. The exosomes were captured by the HER2 aptamer region of MAB immobilized on the chip surface, which enabled the exposure of the G-quadruplex DNA (G4 DNA) that could form peroxidase-like G4-hemin. In turn, the formed G4-hemin catalyzed the deposition of plentiful tyramine-coated gold nanoparticles (AuNPs-Ty) on the exosome membrane with the help of H2O2, generating a significantly enhanced SPR signal. In the reformative TSA system, the horseradish peroxidase (HRP) as a major component was replaced with nonenzymic G4-hemin, bypassing the defects of natural enzymes. Moreover, the dual-recognition of the surface proteins and lipid membrane of the desired exosomes endowed the sensing strategy with high specificity without the interruption of free proteins. As a result, this developed SPR biosensor exhibited a wide linear range from 1.0 × 104 to 1.0 × 107 particles/mL. Importantly, this strategy was able to accurately distinguish HER2-positive breast cancer patients from healthy individuals, exhibiting great potential clinical application. Graphical Abstract

The influence of polyethylene glycol passivation on the surface plasmon resonance induced photothermal properties of gold nanorods

Nanoscale ◽  
2018 ◽  
Vol 10 (28) ◽  
pp. 13684-13693 ◽  
Author(s):  
Ramesh Marasini ◽  
Arunkumar Pitchaimani ◽  
Tuyen Duong Thanh Nguyen ◽  
Jeffrey Comer ◽  
Santosh Aryal
Keyword(s):  
Surface Plasmon Resonance ◽  
Heat Conduction ◽  
Polyethylene Glycol ◽  
Surface Plasmon ◽  
Gold Nanorods ◽  
Plasmon Resonance ◽  
Colloidal Stability ◽  
Surface Passivation ◽  
Liquid Interface ◽  
Comprehensive Study

This comprehensive study suggested an optimum PEG polymer length of 5 kDa is advantageous for surface passivation and colloidal stability of AuNRs, which also maximizes the heat conduction from the nanorod–liquid interface to the surroundings, thereby, enhancing photothermal efficiency.

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