DNA aptamer-based fiber optic biosensor for selective and label-free detection of dopamine

2015 ◽  
Author(s):  
M. I. Zibaii ◽  
H. Latifi ◽  
A. Asadollahi ◽  
A. H. Bayat ◽  
A. Haghparast
Keyword(s):  
Fiber Optic ◽  
Dna Aptamer ◽  
Label Free ◽  
Label Free Detection

Label-Free Detection of Escherichia coli from Mixed Bacterial Cultures Using Bacteriophage T4 on Plasmonic Fiber-Optic Sensor

ACS Sensors ◽  
2021 ◽  
Author(s):  
Pallavi Halkare ◽  
Nirmal Punjabi ◽  
Jigme Wangchuk ◽  
Santhosh Madugula ◽  
Kiran Kondabagil ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fiber Optic ◽  
Bacteriophage T4 ◽  
Fiber Optic Sensor ◽  
Label Free ◽  
Bacterial Cultures ◽  
Optic Sensor ◽  
Label Free Detection

scholarly journals Aptamer Functionalized Lipid Multilayer Gratings for Label-Free Analyte Detection

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2433
Author(s):  
Plengchart Prommapan ◽  
Nermina Brljak ◽  
Troy W. Lowry ◽  
David Van Winkle ◽  
Steven Lenhert
Keyword(s):  
Lipid Composition ◽  
Optical Biosensor ◽  
Optical Signal ◽  
Dna Aptamer ◽  
Critical Parameters ◽  
Label Free ◽  
Scale Thickness ◽  
Label Free Detection ◽  
Analyte Detection ◽  
Micrometer Scale

Lipid multilayer gratings are promising optical biosensor elements that are capable of transducing analyte binding events into changes in an optical signal. Unlike solid state transducers, reagents related to molecular recognition and signal amplification can be incorporated into the lipid grating ink volume prior to fabrication. Here we describe a strategy for functionalizing lipid multilayer gratings with a DNA aptamer for the protein thrombin that allows label-free analyte detection. A double cholesterol-tagged, double-stranded DNA linker was used to attach the aptamer to the lipid gratings. This approach was found to be sufficient for binding fluorescently labeled thrombin to lipid multilayers with micrometer-scale thickness. In order to achieve label-free detection with the sub-100 nm-thick lipid multilayer grating lines, the binding affinity was improved by varying the lipid composition. A colorimetric image analysis of the light diffracted from the gratings using a color camera was then used to identify the grating nanostructures that lead to an optimal signal. Lipid composition and multilayer thickness were found to be critical parameters for the signal transduction from the aptamer functionalized lipid multilayer gratings.

scholarly journals Label-free detection of DNA hybridization with a compact LSPR-based fiber-optic sensor

The Analyst ◽  
2017 ◽  
Vol 142 (11) ◽  
pp. 1974-1981 ◽  
Author(s):  
Savannah Kaye ◽  
Zheng Zeng ◽  
Mollye Sanders ◽  
Krishnan Chittur ◽  
Paula M. Koelle ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Dna Hybridization ◽  
Localized Surface Plasmon Resonance ◽  
Fiber Optic ◽  
Fiber Optic Sensor ◽  
Localized Surface Plasmon ◽  
Label Free ◽  
Optic Sensor ◽  
Label Free Detection

A miniaturized, robust, localized surface plasmon resonance (LSPR)–coupled fiber-optic (FO) nanoprobe providing an integrated and portable solution for detection of DNA hybridization and measurement of DNA concentrations has been demonstrated.

Evanescent wave absorbance based fiber optic biosensor for label-free detection of E. coli at 280nm wavelength

2011 ◽  
Vol 26 (7) ◽  
pp. 3367-3370 ◽  
Author(s):  
Reshma Bharadwaj ◽  
V.V.R. Sai ◽  
Kamini Thakare ◽  
Arvind Dhawangale ◽  
Tapanendu Kundu ◽  
...  
Keyword(s):  
Evanescent Wave ◽  
Fiber Optic ◽  
Label Free ◽  
E Coli ◽  
Label Free Detection
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