scholarly journals Label-free detection of kanamycin based on a G-quadruplex DNA aptamer-based fluorescent intercalator displacement assay

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Yun-Peng Xing ◽  
Chun Liu ◽  
Xiao-Hong Zhou ◽  
Han-Chang Shi
Keyword(s):  
Dna Aptamer ◽  
Label Free ◽  
Quadruplex Dna ◽  
Label Free Detection ◽  
G Quadruplex ◽  
Displacement Assay ◽  
Fluorescent Intercalator Displacement

scholarly journals The Functional Role of Loops and Flanking Sequences of G-Quadruplex Aptamer to the Hemagglutinin of Influenza a Virus

2021 ◽  
Vol 22 (5) ◽  
pp. 2409
Author(s):  
Anastasia A. Bizyaeva ◽  
Dmitry A. Bunin ◽  
Valeria L. Moiseenko ◽  
Alexandra S. Gambaryan ◽  
Sonja Balk ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Core Structure ◽  
Dna Aptamer ◽  
Tertiary Structures ◽  
Quadruplex Dna ◽  
G Quadruplex ◽  
Flanking Sequences ◽  
Related Proteins

Nucleic acid aptamers are generally accepted as promising elements for the specific and high-affinity binding of various biomolecules. It has been shown for a number of aptamers that the complexes with several related proteins may possess a similar affinity. An outstanding example is the G-quadruplex DNA aptamer RHA0385, which binds to the hemagglutinins of various influenza A virus strains. These hemagglutinins have homologous tertiary structures but moderate-to-low amino acid sequence identities. Here, the experiment was inverted, targeting the same protein using a set of related, parallel G-quadruplexes. The 5′- and 3′-flanking sequences of RHA0385 were truncated to yield parallel G-quadruplex with three propeller loops that were 7, 1, and 1 nucleotides in length. Next, a set of minimal, parallel G-quadruplexes with three single-nucleotide loops was tested. These G-quadruplexes were characterized both structurally and functionally. All parallel G-quadruplexes had affinities for both recombinant hemagglutinin and influenza virions. In summary, the parallel G-quadruplex represents a minimal core structure with functional activity that binds influenza A hemagglutinin. The flanking sequences and loops represent additional features that can be used to modulate the affinity. Thus, the RHA0385–hemagglutinin complex serves as an excellent example of the hypothesis of a core structure that is decorated with additional recognizing elements capable of improving the binding properties of the aptamer.

scholarly journals Comparison of DNAzyme activity for the development of an immobilized heme sensor

MRS Advances ◽  
2018 ◽  
Vol 3 (26) ◽  
pp. 1491-1496
Author(s):  
Natalie Hughes ◽  
Nancy Nguyen ◽  
Deanna-Kaye Daley ◽  
Justin Grennell ◽  
Amira Gee ◽  
...  
Keyword(s):  
Self Assembly ◽  
Point Of Care ◽  
Label Free ◽  
Detection Range ◽  
Chemical Complexity ◽  
Detection Strategy ◽  
Detection Approach ◽  
Label Free Detection ◽  
G Quadruplex ◽  
Care Systems

ABSTRACTPoint-of-care systems require highly sensitive, quantitative and selective detection platforms for the real-time multiplexed monitoring of target analytes. To ensure facile development of a sensor, it is preferable for the detection assay to have minimal chemical complexity, contain no wash steps and provide a wide and easily adaptable detection range for multiple targets. Current studies involve label-free detection strategy for relevant clinical molecules such as heme using G-quadruplex based self-assembly. We have explored the measurement of binding and kinetic parameters of various G-quadruplex/heme complexes which are able to self-associate to form a DNAzyme with peroxidase mimicking capabilities and are critical to nucleic acid research. The detection strategy includes immobilizing the G-quadruplex sequences within a polymer matrix to provide a self-assembly based detection approach for heme that could be translated towards other clinically relevant targets.

scholarly journals Aptamer Functionalized Lipid Multilayer Gratings for Label-Free Analyte Detection

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2433
Author(s):  
Plengchart Prommapan ◽  
Nermina Brljak ◽  
Troy W. Lowry ◽  
David Van Winkle ◽  
Steven Lenhert
Keyword(s):  
Lipid Composition ◽  
Optical Biosensor ◽  
Optical Signal ◽  
Dna Aptamer ◽  
Critical Parameters ◽  
Label Free ◽  
Scale Thickness ◽  
Label Free Detection ◽  
Analyte Detection ◽  
Micrometer Scale

Lipid multilayer gratings are promising optical biosensor elements that are capable of transducing analyte binding events into changes in an optical signal. Unlike solid state transducers, reagents related to molecular recognition and signal amplification can be incorporated into the lipid grating ink volume prior to fabrication. Here we describe a strategy for functionalizing lipid multilayer gratings with a DNA aptamer for the protein thrombin that allows label-free analyte detection. A double cholesterol-tagged, double-stranded DNA linker was used to attach the aptamer to the lipid gratings. This approach was found to be sufficient for binding fluorescently labeled thrombin to lipid multilayers with micrometer-scale thickness. In order to achieve label-free detection with the sub-100 nm-thick lipid multilayer grating lines, the binding affinity was improved by varying the lipid composition. A colorimetric image analysis of the light diffracted from the gratings using a color camera was then used to identify the grating nanostructures that lead to an optimal signal. Lipid composition and multilayer thickness were found to be critical parameters for the signal transduction from the aptamer functionalized lipid multilayer gratings.

scholarly journals Label Free Detection of Adenosine Based on Hemin/G-Quadruplex Peroxidase-Mimicking DNAzyme as Bioelectrocatalyst

2013 ◽  
Vol 25 (18) ◽  
pp. 10541-10545 ◽  
Author(s):  
Yu Li ◽  
Sen Li ◽  
Ge-Qing Xia ◽  
Yuansheng Wang
Keyword(s):  
Label Free ◽  
Label Free Detection ◽  
G Quadruplex

Cationic-perylene-G-quadruplex complex based fluorescent biosensor for label-free detection of Pb 2+

Talanta ◽  
2016 ◽  
Vol 149 ◽  
pp. 98-102 ◽  
Author(s):  
Xu-Hua Zhao ◽  
Liang Gong ◽  
Yuan Wu ◽  
Xiao-Bing Zhang ◽  
Jun Xie
Keyword(s):  
Label Free ◽  
Fluorescent Biosensor ◽  
Label Free Detection ◽  
G Quadruplex
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