scholarly journals A versatile fluorescence lifetime imaging system for scanning large areas with high time and spatial resolution

2014 ◽  
Author(s):  
César Bernardo ◽  
Michael Belsley ◽  
Etelvina de Matos Gomes ◽  
Hugo Gonçalves ◽  
Dmitry Isakov ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Fluorescence Lifetime ◽  
Imaging System ◽  
High Time ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

Fluorescence Lifetime Imaging and Fourier Transform Infrared Spectroscopy of Michelangelo's David

2005 ◽  
Vol 59 (9) ◽  
pp. 1174-1181 ◽  
Author(s):  
Daniela Comelli ◽  
Gianluca Valentini ◽  
Rinaldo Cubeddu ◽  
Lucia Toniolo
Keyword(s):  
Fourier Transform ◽  
Fluorescence Lifetime ◽  
Imaging System ◽  
Inorganic Compounds ◽  
Fluorescence Emission ◽  
Chemical Characterization ◽  
Fourier Transform Infrared ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Analytical Measurements

We developed a combined procedure for the analysis of works of art based on a portable system for fluorescence imaging integrated with analytical measurements on microsamples. The method allows us to localize and identify organic and inorganic compounds present on the surface of artworks. The fluorescence apparatus measures the temporal and spectral features of the fluorescence emission, excited by ultraviolet (UV) laser pulses. The kinetic of the emission is studied through a fluorescence lifetime imaging system, while an optical multichannel analyzer measures the fluorescence spectra of selected points. The chemical characterization of the compounds present on the artistic surfaces is then performed by means of analytical measurements on microsamples collected with the assistance of the fluorescence maps. The previous concepts have been successfully applied to study the contaminants on the surface of Michelangelo's David. The fluorescence analysis combined with Fourier transform infrared (FT-IR) measurements revealed the presence of beeswax, which permeates most of the statue surface, and calcium oxalate deposits mainly arranged in vertical patterns and related to rain washing.

Fluorescence lifetime imaging system with nm-resolution and single-molecule sensitivity

2002 ◽  
Author(s):  
Michael Wahl ◽  
Hans-Juergen Rahn ◽  
Uwe Ortmann ◽  
Rainer Erdmann ◽  
Martin Boehmer ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescence Lifetime ◽  
Imaging System ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

All-solid-state diode-pumped fluorescence lifetime imaging system for biomedicine and microscopy

1999 ◽  
Author(s):  
M. J. Cole ◽  
K. Dowling ◽  
R. Jones ◽  
D. Parsons-Karavassilis ◽  
P. M. W. French ◽  
...  
Keyword(s):  
Solid State ◽  
Fluorescence Lifetime ◽  
Imaging System ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Diode Pumped

scholarly journals Video-rate two-photon excited fluorescence lifetime imaging system with interleaved digitization

2015 ◽  
Vol 40 (14) ◽  
pp. 3296 ◽  
Author(s):  
Ximeng Y. Dow ◽  
Shane Z. Sullivan ◽  
Ryan D. Muir ◽  
Garth J. Simpson
Keyword(s):  
Fluorescence Lifetime ◽  
Imaging System ◽  
Fluorescence Lifetime Imaging ◽  
Two Photon ◽  
Lifetime Imaging

A Two-Dimensional Fluorescence Lifetime Imaging System Using a Gated Image Intensifier

1991 ◽  
Vol 45 (3) ◽  
pp. 360-366 ◽  
Author(s):  
Xue Feng Wang ◽  
Teruo Uchida ◽  
David M. Coleman ◽  
Shigeo Minami
Keyword(s):  
Fluorescence Lifetime ◽  
Imaging System ◽  
Image Intensifier ◽  
Fluorescence Lifetime Imaging ◽  
Two Dimensional ◽  
Lifetime Imaging

scholarly journals Endoscopic Fluorescence Lifetime Imaging for In Vivo Intraoperative Diagnosis of Oral Carcinoma

2013 ◽  
Vol 19 (4) ◽  
pp. 791-798 ◽  
Author(s):  
Yinghua Sun ◽  
Jennifer E. Phipps ◽  
Jeremy Meier ◽  
Nisa Hatami ◽  
Brian Poirier ◽  
...  
Keyword(s):  
Head And Neck ◽  
Fluorescence Lifetime ◽  
Imaging System ◽  
Spectral Band ◽  
Average Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
Label Free ◽  
Lifetime Imaging ◽  
Surgical Field

AbstractA clinically compatible fluorescence lifetime imaging microscopy (FLIM) system was developed. The system was applied to intraoperative in vivo imaging of head and neck squamous cell carcinoma (HNSCC). The endoscopic FLIM prototype integrates a gated (down to 0.2 ns) intensifier imaging system and a fiber-bundle endoscope (0.5-mm-diameter, 10,000 fibers with a gradient index lens objective 0.5 NA, 4-mm field of view), which provides intraoperative access to the surgical field. Tissue autofluorescence was induced by a pulsed laser (337 nm, 700 ps pulse width) and collected in the 460 ± 25 nm spectral band. FLIM experiments were conducted at 26 anatomic sites in ten patients during head and neck cancer surgery. HNSCC exhibited a weaker florescence intensity (~50% less) when compared with healthy tissue and a shorter average lifetime (τHNSCC = 1.21 ± 0.04 ns) than the surrounding normal tissue (τN = 1.49 ± 0.06 ns). This work demonstrates the potential of FLIM for label-free head and neck tumor demarcation during intraoperative surgical procedures.

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