scholarly journals Optical spectrometer for a confocal scanning laser microscope with applications in porphyrin-containing specimens

1995 ◽  
Author(s):  
Oliver Trepte ◽  
Ingrid Rokahr ◽  
Stefan Andersson-Engels
Keyword(s):  
Scanning Laser ◽  
Confocal Scanning Laser Microscope ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

Fading of the diaminobenzidine reaction product in the confocal scanning laser microscope

1989 ◽  
Vol 47 ◽  
pp. 146-147
Author(s):  
JS Deitch ◽  
KL Smith ◽  
JW Swann ◽  
JN Turner
Keyword(s):  
Pyramidal Neurons ◽  
Light And Electron Microscopy ◽  
Scanning Laser ◽  
Laser Exposure ◽  
Confocal Scanning Laser Microscope ◽  
Fluorescent Markers ◽  
Before And After ◽  
Staining Protocol ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

Neurons labeled with horseradish peroxidase and reacted with diaminobenzidine (DAB) can be imaged using a confocal scanning laser microscope (CSLM) in the reflection mode. In contrast to fluorescent markers, the DAB reaction product is thought to be stable and can be observed by both light and electron microscopy. We have investigated the sensitivity of the DAB reaction product to laser irradiation, and present the spectrophotometric properties of DAB before and after exposure in the CSLM.Pyramidal neurons in slices of rat hippocampus were injected with biocytin (a biotin-lysine complex), fixed overnight in 4% paraformaldehyde, and vibratome sectioned at 75 μm. Biocytin was detected with avidin-HRP (1:200) in 0.5% Triton X-100, incubated in DAB (0.5 mg/ml) with or without 0.04% nickel ammonium sulfate (Ni), dehydrated, and imaged in a Bio Rad MRC-500 CSLM with an argon ion laser (488 and 514 nm). Spectrophotometric measurements of the soma were made on a Zeiss microspectrophotometer, as a function of laser exposure (100-1000 scans) and staining protocol.

Diagnostic Revolution of Microhematuria by Real Time Confocal Scanning Laser Microscope: Hyodo-Iino-Miyagawa Method, Third Report

Nephron ◽  
1995 ◽  
Vol 70 (2) ◽  
pp. 171-179 ◽  
Author(s):  
Toru Hyodo ◽  
Ikuo Miyagawa ◽  
Akihiro Iino ◽  
Koji Ono ◽  
Tsutomu Kuomi ◽  
...  
Keyword(s):  
Real Time ◽  
Scanning Laser ◽  
Confocal Scanning Laser Microscope ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

Central and peripheral nervous structures as seen at the confocal scanning laser microscope

1994 ◽  
Vol 175 (3) ◽  
pp. 229-237 ◽  
Author(s):  
P. CASTANO ◽  
A. MARCUCCI ◽  
A. MIANI JR ◽  
M. MORINI ◽  
S. VERALDI ◽  
...  
Keyword(s):  
Scanning Laser ◽  
Confocal Scanning Laser Microscope ◽  
Confocal Scanning ◽  
Confocal Scanning Laser
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