Time-resolved imaging system for fluorescence-guided surgery with lifetime imaging capability

2014 ◽  
Author(s):  
F. Powolny ◽  
K. Homicsko ◽  
R. Sinisi ◽  
Claudio E. Bruschini ◽  
E. Grigoriev ◽  
...  
Keyword(s):  
Imaging System ◽  
Time Resolved ◽  
Imaging Capability ◽  
Lifetime Imaging ◽  
Guided Surgery ◽  
Fluorescence Guided Surgery ◽  
Time Resolved Imaging

scholarly journals Indocyanine green matching phantom for fluorescence-guided surgery imaging system characterization and performance assessment

2020 ◽  
Vol 25 (05) ◽  
pp. 1
Author(s):  
Alberto J. Ruiz ◽  
Mindy Wu ◽  
Ethan Philip M. LaRochelle ◽  
Dimitris Gorpas ◽  
Vasilis Ntziachristos ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Performance Assessment ◽  
Imaging System ◽  
Guided Surgery ◽  
Fluorescence Guided Surgery ◽  
System Characterization ◽  
And Performance

Applications and Techniques for 2D Picosecond Imaging for Circuit Analysis

2014 ◽  
Author(s):  
Franco Stellari ◽  
Peilin Song ◽  
Alan J. Weger
Keyword(s):  
Spectral Analysis ◽  
Reverse Engineering ◽  
Near Infrared ◽  
Circuit Analysis ◽  
Time Resolved ◽  
Imaging Capability ◽  
Specific Focus ◽  
Time Resolved Imaging ◽  
Vlsi Chips

Abstract In this paper, we present the latest results obtained with a 2D Picosecond Imaging Circuit Analysis (PICA) camera with enhanced Near InfraRed (NIR) sensitivity [1] for taking 2D Time Resolved Emission (TRE). We will discuss key applications where the time-resolved imaging capability is very effective in reducing the debug time and improving the interpretation of the failure signatures of several VLSI chips. Besides conventional chip diagnostics, specific focus will be dedicated to new areas of applications, such as security and reverse engineering [2]. We will also discuss spectral analysis and other techniques that can be used to extract valuable information from the PICA dataset.

A hand-held portable imaging system for effective fluorescence-guided surgery of a pancreatic patient-derived orthotopic xenograft (PDOX) in nude mice.

2014 ◽  
Vol 32 (15_suppl) ◽  
pp. e15219-e15219
Author(s):  
Yukihiko Hiroshima ◽  
Ali Maawy ◽  
Fuminari Uehara ◽  
Shinji Miwa ◽  
Shuya Yano ◽  
...  
Keyword(s):  
Nude Mice ◽  
Imaging System ◽  
Guided Surgery ◽  
Orthotopic Xenograft ◽  
Fluorescence Guided Surgery

Influence of dexamethasone on visible 5-ALA fluorescence and quantitative protoporphyrin IX accumulation measured by fluorescence lifetime imaging in glioblastomas: is pretreatment obligatory before fluorescence-guided surgery?

2021 ◽  
pp. 1-9
Author(s):  
Lisa I. Wadiura ◽  
David Reichert ◽  
Veronika Sperl ◽  
Alexandra Lang ◽  
Barbara Kiesel ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Aminolevulinic Acid ◽  
Ex Vivo ◽  
Protoporphyrin Ix ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Guided Surgery ◽  
Fluorescence Guided Surgery ◽  
Significant Difference ◽  
The Mean

OBJECTIVE Fluorescence-guided surgery using 5-aminolevulinic acid (5-ALA) is nowadays widely applied for improved resection of glioblastomas (GBMs). Initially, pretreatment with dexamethasone was considered to be essential for optimal fluorescence effect. However, recent studies reported comparably high rates of visible fluorescence in GBMs despite absence of dexamethasone pretreatment. Recently, the authors proposed fluorescence lifetime imaging (FLIM) for the quantitative analysis of 5-ALA–induced protoporphyrin IX (PpIX) accumulation. The aim of this study was thus to investigate the influence of dexamethasone on visible fluorescence and quantitative PpIX accumulation. METHODS The authors prospectively analyzed the presence of visible fluorescence during surgery in a cohort of patients with GBMs. In this study, patients received dexamethasone preoperatively only if clinically indicated. One representative tumor sample was collected from each GBM, and PpIX accumulation was analyzed ex vivo by FLIM. The visible fluorescence status and mean FLIM values were correlated with preoperative intake of dexamethasone. RESULTS In total, two subgroups with (n = 27) and without (n = 20) pretreatment with dexamethasone were analyzed. All patients showed visible fluorescence independent from preoperative dexamethasone intake. Furthermore, the authors did not find a statistically significant difference in the mean FLIM values between patients with and without dexamethasone pretreatment (p = 0.097). CONCLUSIONS In this first study to date, the authors found no significant influence of dexamethasone pretreatment on either visible 5-ALA fluorescence during GBM surgery or PpIX accumulation based on FLIM. According to these preliminary data, the authors recommend administering dexamethasone prior to fluorescence-guided surgery of GBMs only when clinically indicated.

Time-resolved imaging of fluorescent inclusions in optically turbid medium — phantom study

2010 ◽  
Vol 18 (1) ◽  
Author(s):  
M. Kacprzak ◽  
A. Liebert ◽  
P. Sawosz ◽  
N. Żołek ◽  
D. Milej ◽  
...  
Keyword(s):  
Optical Properties ◽  
Imaging System ◽  
Single Photon ◽  
Photon Counting ◽  
Turbid Medium ◽  
Time Resolved ◽  
Excitation Light ◽  
Time Resolved Imaging ◽  
Fish Tank ◽  
Surrounding Liquid

AbstractWe present results of application of a time-resolved optical system for imaging of fluorescence excited in an inclusion containing indocyanine green (ICG), and located in optically turbid medium. The developed imaging system enabled simultaneous acquisition of fluorescence and diffusive reflectance. Eight independent time-resolved measurement channels based on time-correlated single photon counting technique were applied. In four of these channels, used for the fluorescence detection, sets of filters were applied in order to block the excitation light. Fast optomechanical switches allowed us to illuminate sequentially nine different spots on the surface of the studied object and finally 4×4 pixels maps at excitation and emission wavelengths were obtained. A liquid phantom used in this study consists of the fish tank filed with a solution ofmilk and water with black ink added to obtain optical properties in the range of the optical properties typical for the living tissue. A gel ball of a diameter of 5 mm with precisely controlled concentration of ICG was immersed in the liquid. The measurements were performed for inclusion located at different depths and for various ICG concentrations in the gel ball and in the surrounding liquid. The recorded distributions of times of arrival (DTA) of fluorescence photons and times of flight (DTOF) of diffusely reflected photons were analyzed by calculation of their statistical moments. We observed specific changes in moments of the measured DTAs as a function of depth of immersion of the fluorescent inclusion in the medium. We noted also that the changes of moments depend significantly on concentration of the dye in the fluorescence inclusion as well as in the surrounding liquid.

Hyperspectral, Time-Resolved, Fluorescence Imaging System for Large Sample Sizes: Part II. Detection of Fecal Contamination on Spinach Leaves

2018 ◽  
Vol 61 (2) ◽  
pp. 391-398 ◽  
Author(s):  
Kevin Tewey ◽  
Alan M. Lefcourt ◽  
Uri Tasch ◽  
Patrick Shilts ◽  
Moon S. Kim
Keyword(s):  
Fluorescence Imaging ◽  
Imaging System ◽  
Fecal Contamination ◽  
Fluorescence Decay ◽  
Decay Rates ◽  
Time Resolved ◽  
Surface Areas ◽  
Fluorescence Imaging System ◽  
Time Resolved Imaging ◽  
Spinach Leaves

Abstract. To reduce the risk of foodborne illnesses resulting from fecal contamination in produce fields, a hyperspectral, line-scan, laser-induced fluorescence imaging system was developed with the goal of eventually incorporating the imaging system into a pre-harvest detection apparatus for fecal contamination. The imaging system includes an intensified, gated camera, a spectral adapter, a 355 nm pulsed laser, and laser expansion optics that produce a line-illumination profile. To validate and test the system, spinach leaves inoculated with dilutions of bovine fecal material were imaged repeatedly using a predefined set of imaging parameters. These images were used to evaluate methods for detecting fecal contamination. Methods investigated included ratio, edge, threshold, and slope detection. Differences in the magnitude of averaged intensities for the spectral range of 450-500 nm for regions within fecal contamination sites and in nearby uncontaminated surface areas suggested that the 450-500 nm waveband would be a good region for use in detection tests. Validation tests that used threshold or slope detection, the 450-500 nm waveband, and that took advantage of the slower fluorescence decay rates of fecal contamination sites relative to uncontaminated surface areas showed almost 100% detection of 1:2, 1:10, and 1:100 dilution sites and over 70% detection of 1:200 dilution sites with essentially zero false positives. These results suggest that the imaging system has potential for development of a commercially viable apparatus for pre-harvest detection of fecal contamination in produce fields and for detection of fecal contamination of leafy green vegetables in general. Keywords: Fecal detection, Fluorescence imaging, Food safety, Machine vision, Spinach, Time-resolved imaging.

A Scanning Beam Time-Resolved Imaging System for Fingerprint Detection

2000 ◽  
Vol 45 (3) ◽  
pp. 14729J ◽  
Author(s):  
Robert D. Roorda ◽  
Alfonso C. Ribes ◽  
Savvas Damaskinos ◽  
A.E. (Ted) (Ted) Dixon ◽  
E. Roland Menzel
Keyword(s):  
Imaging System ◽  
Time Resolved ◽  
Scanning Beam ◽  
Time Resolved Imaging ◽  
Fingerprint Detection
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